The general transcription factors TFIIE and TFIIH assemble at the transcription start site with RNA Polymerase II. Here the authors provide evidence that the TFIIEα and TFIIEβ subunits anchor the TFIIH kinase module within the preinitiation complex before their release during transcription

Carlos M. Genes

Cathy Braun

Emmanuel Compe

Frederic Coin

Jean-Marc Egly

Nature Communications

International audienceIn eukaryotes, the general transcription factors TFIIE and TFIIH assemble at the transcription start site with RNA Polymerase II. However, the mechanism by which these transcription factors incorporate the preinitiation complex and coordinate their action during RNA polymerase II transcription remains elusive. Here we show that the TFIIEα and TFIIEβ subunits anchor the TFIIH kinase module (CAK) within the preinitiation complex. In addition, we show that while RNA polymerase II phosphorylation and DNA opening occur, CAK and TFIIEα are released from the promoter. This dissociation is impeded by either ATP-γS or CDK7 inhibitor THZ1, but still occurs when XPB activity is abrogated. Finally, we show that the Core-TFIIH and TFIIEβ are subsequently removed, while elongation factors such as DSIF are recruited. Remarkably, these early transcriptional events are affected by TFIIE and TFIIH mutations associated with the developmental disorder, trichothiodystrophy

Compe, Emmanuel

Genes, Carlos

Braun, Cathy

Coin, Frédéric

Egly, Jean-Marc

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TFIIE orchestrates the recruitment of the TFIIH kinase module at promoter before release during transcription

http://link.springer.com/article/10.1038/s41467-019-10131-1

TFIIE orchestrates the recruitment of the TFIIH kinase module at promoter before release during transcription

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