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Previous issue date: 2016Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Arboviruses and Insect Vectors. Paris, France.Institut Pasteur. Arboviruses and Insect Vectors. Paris, France.Centre de Démoustication/Collectivité Territoriale de La Martinique. Fort-de-France, Martinique.Institut Pasteur of French Guiana. Unit of Medical Entomology, Cayenne, French Guiana.Institut Pasteur of Guadeloupe. Laboratory of Medical Entomology, Environment and Health. Les Abymes, Guadeloupe.Institut Pasteur of New Caledonia. Dengue and Arboviruses Research and Expertise Unit. Nouméa, New Caledonia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Arboviruses and Insect Vectors. Paris, France.Background
Since the major outbreak in 2007 in the Yap Island, Zika virus (ZIKV) causing dengue-like
syndromes has affected multiple islands of the South Pacific region. In May 2015, the virus
was detected in Brazil and then spread through South and Central America. In December
2015, ZIKV was detected in French Guiana and Martinique. The aim of the study was to
evaluate the vector competence of the mosquito spp. Aedes aegypti and Aedes albopictus
from the Caribbean (Martinique, Guadeloupe), North America (southern United States),
South America (Brazil, French Guiana) for the currently circulating Asian genotype of ZIKV
isolated from a patient in April 2014 in New Caledonia.
Methodology/Principal Findings
Mosquitoes were orally exposed to an Asian genotype of ZIKV (NC-2014-5132). Upon
exposure, engorged mosquitoes were maintained at 28°±1°C, a 16h:8h light:dark cycle and
80% humidity. 25–30 mosquitoes were processed at 4, 7 and 14 days post-infection (dpi).
Mosquito bodies (thorax and abdomen), heads and saliva were analyzed to measure infection,
dissemination and transmission, respectively. High infection but lower disseminated
infection and transmission rates were observed for both Ae. aegypti and Ae. albopictus. Ae.
aegypti populations from Guadeloupe and French Guiana exhibited a higher dissemination
of ZIKV than the other Ae. aegypti populations examined. Transmission of ZIKV was
observed in both mosquito species at 14 dpi but at a low level.Conclusions/Significance
This study suggests that although susceptible to infection, Ae. aegypti and Ae. albopictus
were unexpectedly low competent vectors for ZIKV. This may suggest that other factors
such as the large naïve population for ZIKV and the high densities of human-biting mosquitoes
contribute to the rapid spread of ZIKV during the current outbreak