Hydrocarbon-oxidizing bacteria were identified from three stations on DeGray Reservoir, Arkansas. The organisms were primarily gram-negative rods representing 9 taxa and 37 biotypes Pseudomonas spp. were the most common isolates. The largest populations were found in areas most frequently used by boaters, although seasonal fluctuations were apparent during the spring and fall. The degradation of outboard motor oil by the five most rapidly growing isolates was studied. Each species had a different decomposition profile, and substrate oxidation rates were variable Acinetobacter calcoaceticus var. anitratus was the most efficient decomposer