Ocho imágenes de un meningioma infiltrante en una paciente de 49 años.Eight pictures of an infiltrating meningioma in a 49-year-old female patient

Anderson CMH

Thwaites DT

Siete imágenes de un meningioma infiltrante en un paciente de 33 años.Seven pictures of an infiltrating meningioma in a 33-year-old male patient

Iglesias Rozas, José Rafael, 1942-

Gestion del Repositorio Documental de la Universidad de Salamanca

Meningioma infiltrante

Imagen de un meningioma infiltrante en una paciente de 39 años.Picture of an infiltrating meningioma in a 39-year-old female patient

A H+-coupled amino acid transporter has been characterised functionally at the brush border membrane of the human intestinal cell line Caco-2. This carrier, hPAT1 (human Proton-coupled Amino acid Transporter 1)or SLC36A1, has been identified recently at the molecular level and hPAT1 protein is localised to the brush border membrane of human small intestine. hPAT1 transports both amino acids (e.g., β-alanine) and therapeutic agents (e.g., o-cycloserine). In human Caco-2 cells, hPAT1 function (H+/amino acid symport) is associated with a decrease in intracellular pH (pHi), which selectively activates the Na+AH+ exchanger NHE3, and thus maintains pHi and the driving force for hPAT1 function (the H+ electrochemical gradient). This study provides the first evidence for regulation of hPAT1 function. Activation of the cAMP/protein kinase A pathway in Caco-2 cell monolayers either using pharmacological tools (forskolin, 8-br-cAMP, [11,22,28Ala]VIP) or physiological activators (the neuropeptides VIP and PACAP) inhibited hPAT1 function (β-alanine uptake) at the apical membrane. Under conditions where NHE3 is inactive (the absence of Na+, apical pH 5.5, the presence of the NHE3 inhibitor S1611) no regulation of β-alanine uptake is observed. Forskolin and VIP inhibit pH, recovery (NHE3 function) from β-alanine-induced intracellular acidification. Immunocytochemistry localises NHERF1 (NHE3 regulatory factor 1) to the apical portion of Caco-2 cells where it will interact with NHE3 and allow PKA-mediated phosphorylation of NHE3. In conclusion, we have shown that amino acid uptake via hPAT1 is inhibited by activators of the cAMP pathway indirectly through inhibition of NHE3 activity. \ua9 2005 Wiley-Liss, Inc

Newcastle University E-Prints

Indirect regulation of the intestinal H<sup>+</sup>-coupled amino acid transporter hPAT1 (SLC36A1)

Cuatro imágenes de un meningioma infiltrante.Four pictures of an infiltrating meningioma

Dos imágenes de un meningioma infiltrante en un paciente de 32 años.Two pictures of an infiltrating meningioma in a 32-year-old male patient

Dos imágenes de un meningioma infiltrante en una paciente de 72 años.Two pictures of an infiltrating meningioma in a 72-year-old female patient

Dos imágenes de un meningioma infiltrante.Two pictures of an infiltrating meningioma

Imagen de un meningioma infiltrante en un paciente de 66 años.Picture of an infiltrating meningioma in a 66-year-old male patient

Tres imágenes de un meningioma infiltrante.Three pictures of an infiltrating meningioma

Tres imágenes de un meningioma infiltrante en un paciente de 61 años.Three pictures of an infiltrating meningioma in a 61-year-old male patient

Indirect regulation of the intestinal H<sup>+</sup>-coupled amino acid transporter hPAT1 (SLC36A1)

Abstract

Similar works

Full text

Available Versions

Gestion del Repositorio Documental de la Universidad de Salamanca

Gestion del Repositorio Documental de la Universidad de Salamanca

Newcastle University E-Prints

Gestion del Repositorio Documental de la Universidad de Salamanca

Gestion del Repositorio Documental de la Universidad de Salamanca

Gestion del Repositorio Documental de la Universidad de Salamanca

Gestion del Repositorio Documental de la Universidad de Salamanca

Gestion del Repositorio Documental de la Universidad de Salamanca

Gestion del Repositorio Documental de la Universidad de Salamanca

Gestion del Repositorio Documental de la Universidad de Salamanca