Cazzaniga, Clarissa

Dechesne, Arnaud

Plósz, Benedek G.

Steidl, Michael

Valverde Pérez, Borja

Wágner, Dorottya Sarolta

Online Research Database In Technology

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Downloaded from orbit.dtu.dk on: Dec 18, 2017Impact of influent quality on green microalgal cultivation with used water resources –experimental assessment combined with image analysisWágner, Dorottya Sarolta; Valverde Pérez, Borja; Cazzaniga, Clarissa; Steidl, Michael ; Dechesne,Arnaud; Plósz, Benedek G.Publication date:2016Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Wágner, D. S., Valverde Pérez, B., Cazzaniga, C., Steidl, M., Dechesne, A., & Plósz, B. G. (2016). Impact ofinfluent quality on green microalgal cultivation with used water resources – experimental assessment combinedwith image analysis. Poster session presented at MEWE and biofilms IWA specialist conference, Copenhagen,Denmark. 1. INTRODUCTION   2. OBJECTIVES   3.MATERIALS AND METHODS   5. CONCLUSION   ACKNOWLEDGEMENT   4. RESULTS  • New technologies are developed to recover wastewater resources → Potential resource recovery (water, nutrients, energy) using microalgae • Resource recovery through a two-stage bacterial-algal system: • Enhanced biological phosphorus removal and recovery system (EBP2R) to produce green microalgal growth medium with optimised N-to-P ratios[1] • Optimal algal cultivation, thereby intracellularly storing N and P  • Test the impact of the expected variability of the EBP2R effluent quality on the algal cultivation and nutrient removal • Develop a method to quantify different microalgal species within a mixed consortium using microscopy and image analysis  Impact of influent quality on green microalgal cultivation with used water resources – experimental assessment combined with image analysis  Dorottya Sarolta Wágner*, Borja Valverde-Pérez, Clarissa Cazzaniga, Michael Steidl, Arnaud Dechesne, Benedek Gy. Plósz *dosaw@env.dtu.dk,  DTU Environment, Department of Environmental Engineering,  Technical University of Denmark, Miljøvej, Building 113, 2800 Kgs. Lyngby, DENMARK • N-to-P ratio is a powerful tool for microbial community control in open PBRs cultivating mixed consortia • Contamination by Diatoms can deteriorate the process performance • PBR cultivating monoculture of Chlorella sp. can be operated without contamination • EBP2R can support optimal algal growth  EBP2R performance: Control system fails to keep the optimal N-to-P ratio when the nitrogen influent load is low [2]  • This variation of the N-to-P ratio can: • Deteriorate PBR performance  • Alter the microalgal community grown in the PBR  Photobioreactor performance assessed in lab-scale reactors: • 1.4 L open reactors treating the effluent of an EBPR with varying N-to-P ratio • Two cultivation scenarios: • Mixed microalgal consortium of Chlorella sp. and Scenedesmus sp. • Monoculture of Chlorella sp. Microbial diversity analysis and quantification via novel image analysis tool: shape recognition • Chlorella sp. (round and small individual cells) • Scenedesmus sp. (elongated cells arranged in two/four-cell colonies) • Diatoms (large, elongated single cells) Photobioreactor performance with mixed microalgal consortium  • Culture contamination after shifting the N-to-P ratio to 5 by Diatoms present in the influent • Poor phosphorus removal (40%) at low N-to-P ratio (due to presence of Diatoms) • After the N-to-P ratio was shifted back to 17 the Diatoms were washed out and consequently the P removal was recovered    Photobioreactor performance with monoculture of Chlorella sp.  • Stable microbial community throughout the cultivation: Chlorella sp. (100%) • Stable phosphorus removal  • Enhanced nitrogen removal after the nitrogen limited period  Scenedesmus sp. Chlorella sp. Diatoms N/P= 16.8±2 N/P= 17.9±0.5 N/P= 10±0.3 N/P= 17.6±1.6 N/P= 16.5±0.1 N/P= 5.2 References: [1] Valverde-Pérez, B., Ramin, E., Smets, B.F., and Plósz, B. Gy., 2015. EBP2R – An innovative enhanced biological nutrient recovery activated sludge system to produce growth medium for green microalgae cultivation. Water Research, 68, 821-830 [2] Valverde-Pérez, B., Fuentes-Martínez, J.M., Flores-Alsina, X., Gernaey, K.V., Huusom, J.K., Plósz, B.Gy., 2016. Control structure design for resource recovery using the enhanced biological phosphorus removal and recovery (EBP2R) activated sludge process. Chemical Engineering Journal, 296, 447-457      

Impact of influent quality on green microalgal cultivation with used water resources – experimental assessment combined with image analysis

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Impact of influent quality on green microalgal cultivation with used water resources – experimental assessment combined with image analysis

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