Andersen, Birgitte

Olsen, Kresten Jon Kromphardt

The filamentous fungal genus Stemphylium (Anamophic Pleospora) is often found on various crops, and especially the common animal feed plant Medicago sativa (alfalfa) is often infected by this plant pathogen. With this in mind it is important to consider what consequences such a contamination can have, e.g. production of mycotoxins. (Firsvad et al. (2009)) A clade of Stemphylium spp. i.e. S. herbarum, S. alfalfae, S. sedicola, S. tomatonis and S. vesicariumare troublesome to distinguish as they share both morphological and molecular characteristics. This study has focused on using chemotaxonomy via HPLC-UV/VIS-MS analysis as a third method for identification and to investigate the chemical potential of the genus Stemphylium. A total of 253 unique compounds were used for chemotaxonomy and the majority of these were unknown compounds

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Downloaded from orbit.dtu.dk on: Dec 18, 2017Chemotaxonomy of the genus StemphyliumOlsen, Kresten Jon Kromphardt; Andersen, BirgittePublication date:2016Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Olsen, K. J. K., & Andersen, B. (2016). Chemotaxonomy of the genus Stemphylium. Poster session presented at9th Joint Natural Products Conference 2016, Copenhagen, Denmark.Chemotaxonomy	gave	some	resolution	of	group	C.	Identification	via	phylogeny	would	give	more	resolution	but	a	total	of	8	chemical	groups	were	identified.	Some	were	of	known	isolates	e.g.	S.	gracilariae,	S.	majusculum and	S.	eturmiunum.Interestingly	S.	lycopersici (group	A)	and	S.	callistephi (group	D)	clustered	within	group	C.	Group	CGroup	A,	B,	D,	E,	FKresten	Jon	Kromphardt	Olsen and	Birgitte AndersenDepartment	of	Biotechnology	and	Biomedicine	Technical	university	of	Denmark,	Kgs.	Lyngby,	DenmarkFrisvad JC,	Andersen	B,	Thrane U.	The	use	of	secondary	metabolite	profiling	in	chemotaxonomy	of	filamentous	fungi. Mycological	research	2008;	112:	231-240The	filamentous	fungal	genus	Stemphylium (AnamophicPleospora) is	often	found	on	various	crops,	and	especially	the	common	animal	feed	plant	Medicago sativa (alfalfa)	is	often	infected	by	this	plant	pathogen.	With	this	in	mind	it	is	important	to	consider	what	consequences	such	a	contamination	can	have,	e.g.	production	of	mycotoxins.(Firsvad et	al.	(2009))	A	clade	of	Stemphylium spp.	i.e.	S.	herbarum,	S.	alfalfae,	S.	sedicola,	S.	tomatonis and	S.	vesicarium are	troublesome		to	distinguish	as	they	share	both	morphological	and	molecular	characteristics.	This	study	has	focused	on	using	chemotaxonomy	via	HPLC-UV/VIS-MS	analysis	as	a	third	method	for	identification	and	to	investigate	the	chemical	potential	of	the	genus	Stemphylium.	A	total	of	253	unique	compounds	were	used	for	chemotaxonomy	and	the	majority	of	these	were	unknown	compounds.	AbstractThe	morphology	of	Stemphylium conidia.	Some	species	require	special	knowledge	of	the	morphology	(I-VI)	to	identify,	while	others	are	easier	to	identify	by	this	method	(VII-IX).Figure	1.	Comparison	of	micromorphology	within	the	Stemphylium genus.	I	– S.	eturmiunum, II	– S.	herbarum,		III	– S.	astragli,	IV	– S.	gracilareae,	V – S.	alfalfae,	VI	– S.	majusculum,	VII	– S.	loti,	VIII	– S.	sarciniforme and								IX	– S.	lancipes. Same	scale	bar	for	all	pictures.Morphological	identificationChemical	identificationCoefficient0.38 0.54 0.69 0.85 1.00CBS714.68-SbotMWFIP023-Ssp FIP026-Ssp FIP066-Ssp FIP107-Ssp FIP289-Ssp FIP292-Ssp IBT8231-Setu CBS714.68-Ssp FIP156-Slyc FIP112-Ssp FIP157-Ssp FIP163-Ssp FIP147-Sast FIP149-Sast FIP173-Ssp FIP242-Ssp FIP266-Ssp IBT8213-Setu IBT40618-Ssp FIP080-Ssp IBT8224-Setu FIP109-Setu FIP129-Smaj IBT8223-Smaj FIP015-Ssp FIP035-Ssp FIP222-Ssp FIP227-Ssp NN009784-Ssp FIP230-Ssp CBS192.86-Salf NN003467-Ssp BA570-Ssp IBT9032-Ssp CBS715.68-Sves BA463-Ssp BA608-Ssp NN042469-Ssp BA1399-Ssp IBT7159-Ssp BA516-Ssp IBT7161-Ssp IBT8230-Sves FIP110-Ssp FIP152-Salf FIP146-Salf FIP113-Ssp FIP165-Ssp BA2319-Ssp IBT10199-Ssp FIP178-Ssp FIP179-Ssp FIP181-Ssp FIP182-Ssp FIP180-Ssp IBT8220-Ssp FIP057-Ssp FIP151-Salf FIP151-Salf IBT8214-Ssp FIP184-Sher CBS191.86-Ssp CBS482.90-Sgra FIP001-Ssp FIP003-Ssp FIP084-Ssp IBT8227-Sgra FIP076-Ssp FIP083-Ssp FIP145-Ssp FIP166-Scal CBS101217-Slan FIP153-Slan CBS716.68-Sglo FIP191-Ssp FIP220-Ssp FIP108-Ssp FIP186-Sglo CBS408.54-Ssol FIP125-Ssol FIP137-Ssol FIP138-Ssol FIP140-Stri FIP241-Stri FIP141-Stri FIP194-Stri FIP197-Stri FIP170-Ssar IBT8221-Ssar FIP238-Ssar IBT8217-Ssar FIP162-Slot NN000638-Slot FIP175-Slot FIP174-Slot FIP217-Slot Examples	of	compoundsConclusion	and	perspectivesChemotaxonomy	with	the	genus	of	Stemphylium gave	a	new	tool	for	identification	of	the	individual	species.	Also,	a	list	of	detected	compounds	within	the	genus	has	been	made.	With	this	list	it	is	possible	to	mine	for	novel	bioactivities	as	it	is	possible	to	analyze	vastly	different	metabolic	profiles	within	the	same	genus.Alterporriol GGroup	C,	S.	solani and	S.	globuliferumAltersolanol MS.	solani markerPyrenophorolS.	loti	markerAK-toxin	IIS.	trifolii markerStemphylinStemphylium markerAll	species	of	this	group	are	very	easy	to	distinguish	from	group	C.	These	also	have	solid	separation	and	many	unique	compounds	to	differentiate	them.	Email:	krjko@dtu.dkReferencesThe	majority	of	known	Stemphylium compounds	are	of	polyketide	origin.	CurvularinGroup	CDihydrotentoxinGroup	C

Chemotaxonomy of the genus <i>Stemphylium</i>

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Chemotaxonomy of the genus <i>Stemphylium</i>

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