Benson

Blank

Gasson

Koebmann

Lechardeur

Mazzoli

Solem

Terzaghi

Wallace

Wang

Westerfeld

Xiao

Zhang

Jianming Liu

Christian Solem

Peter Ruhdal Jensen

Crossref

Biotechnology and Bioengineering

Integrating biocompatible chemistry and manipulating cofactor partitioning in metabolically engineered<i>Lactococcus lactis</i>for fermentative production of (3<i>S</i>)-acetoin

Biocompatible chemistry (BC), i.e. non-enzymatic chemical reactions compatible with living organisms, is increasingly used in conjunction with metabolically engineered microorganisms for producing compounds that do not usually occur naturally. Here we report production of one such compound, (3S)-acetoin, a valuable precursor for chiral synthesis, using a metabolically engineered Lactococcus lactis strain growing under respiratory conditions with ferric iron serving as a BC component. The strain used has all competing product pathways inactivated, and an appropriate cofactor balance is achieved by fine-tuning the respiratory capacity indirectly via the hemin concentration. We achieve high-level (3S)-acetoin production with a final titer of 66 mM (5.8 g/L) and a high yield (71% of the theoretical maximum). To the best of our knowledge, this is the first report describing production of (3S)-acetoin from sugar by microbial fermentation, and the results obtained confirm the potential that lies with BC for producing useful chemicals

Liu, Jianming

Solem, Christian

Jensen, Peter Ruhdal

Online Research Database In Technology

Integrating biocompatible chemistry and manipulating cofactor partitioning in metabolically engineeredLactococcus lactisfor fermentative production of (3S)-acetoin

http://orbit.dtu.dk/ws/files/124360031/Liu_et_al_2016_Biotechnology_and_Bioengineering.pdf

Integrating biocompatible chemistry and manipulating cofactor partitioning in metabolically engineeredLactococcus lactisfor fermentative production of (3S)-acetoin

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Crossref

Online Research Database In Technology