Culture-based detection of nontuberculous Mycobacteria (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust

Bastian, Ivan

Burr, Lucy D.

Choo, Jocelyn M.

Goddard, Thomas R.

Leong, Lex E. X.

Rogers, Geraint B.

Scoleri, Gianny P.

Shephard, Lisa

Thomson, Rachel M.

PubMed

ABSTRACT
          
            Culture-based detection of nontuberculous
            Mycobacteria
            (NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.
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Gianny P. Scoleri

Jocelyn M. Choo

Lex E. X. Leong

Thomas R. Goddard

Lisa Shephard

Lucy D. Burr

Ivan Bastian

Rachel M. Thomson

Geraint B. Rogers

Crossref

Journal of Clinical Microbiology

Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples

University of Queensland eSpace

Culture-independent detection of nontuberculous mycobacteria in clinical respiratory samples

http://espace.library.uq.edu.au/view/UQ:406289/UQ406289_OA.pdf

Culture-independent detection of nontuberculous mycobacteria in clinical respiratory samples

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University of Queensland eSpace