Cottalorda, A

Croustillères, F

Dahyot, S

Jumas-Bilak, E.

Lebeurre, J

Pestel-Caron, M.

Réveillon, M

Soares, A

HAL-IRD

Phenotypic and molecular diversity of urinary isolates of Pseudomonas aeruginosa

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Phenotypic and molecular diversity of urinary isolates of
Pseudomonas aeruginosa
A Cottalorda, S Dahyot, J Lebeurre, A Soares, M Réveillon, F Croustillères,
E. Jumas-Bilak, M. Pestel-Caron
To cite this version:
A Cottalorda, S Dahyot, J Lebeurre, A Soares, M Réveillon, et al.. Phenotypic and molecular diversity
of urinary isolates of Pseudomonas aeruginosa. ECCMID, Apr 2018, Madrid, Spain. ￿hal-02265127￿
ABSTRACT
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OBJECTIVES
LOGO
Phenotypic and molecular diversity 
of urinary isolates of Pseudomonas aeruginosa
Cottalorda A1, Dahyot S1,2, Lebeurre J1, Soares A1,2, Réveillon M2, Croustillères F2, Jumas-Bilak E3, Pestel-Caron M1,2
1 Microbiological Laboratory GRAM EA2656, Rouen University, FRANCE;  2 Bacteriological Laboratory, Rouen University Hospital, FRANCE;  3  Microbiological Laboratory UMR 5569 Hydrosciences, Montpellier University, FRANCE.
P2138
Background
Pseudomonas aeruginosa (PA) is an opportunistic pathogen causing
frequent healthcare associated Urinary Tract Infection (UTI). Due to a
range of mechanisms for adaptation and antibiotic resistance, PA
infections are difficult to treat and often associated with increased
morbidity or mortality. Many studies explored the diversity of cystic
fibrosis strains but little is known about the diversity of urinary strains.
Thus, the aim of our study was to describe phenotypic and molecular
diversity:
• (i) of isolates causing Asymptomatic Bacteriuria (AB) or UTI and ;
• (ii) between isolates from a same sample.
Results
177 isolates were phenotypically and genetically characterized.
Phenotypic diversity
78% of our isolates were Non-MultiDrug Resistant (MDR) (isolates resistant to less than 3
antibiotic families), 16% were MDR (isolates resistant to at least 3 antibiotic families) and 6%
were Extensively Drug Resistant (XDR) (isolates resistant to at least 6 antibiotic families) (Fig.1).
The rate of antibiotic resistance varied between 8% (for ceftazidime) and more than 30%
(fluoroquinolones) (Fig.2). The resistance phenotypes were different between isolates collected
from 10 of the 58 urine samples.
Molecular diversity
MLST identified 34 Sequence Types (STs) for the 58 urine samples. Eight STs were identified for
at least 2 patients. As previously described [1], ST235 , ST175 , and ST111 were associated with
antibiotic resistance (MDR or XDR profiles) (Fig. 3) and known as high-risk clones like ST253 and
ST395. STs of isolates from a given urine sample were different for only 3 patients. Of note, no ST
was specifically associated with AB or UTI (Fig. 4).
0 10 20 30 40
Ticarcillin
Ticarcillin - Clavulanate
Piperacillin
Piperacillin - Tazobactam
Ceftazidime
Cefepime
Aztreonam
Imipenem
Meropenem
Gentamicine
Tobramycine
Amikacine
Levofloxacine
Ciprofloxacine
Fig. 2  Antibiotic resistance (%) of the urinary 
isolates  of P. aeruginosa
Fig. 1  Antibiotic susceptibility profile of 
the urinary isolates of P. aeruginosa (%)
Fig.3  MST of the STs depending on the antibiotic 
susceptibility profile
Fig.4  MST of the STs depending on the clinical data
Materials and methods
We studied 2 to 5 isolates of PA from 58 urine samples (38 AB and 20
UTI) sent to the Rouen University Hospital from June through
November 2016. More than one urine sample were analyzed for 5 of
the 51 patients included.
Antibiotic susceptibility was studied by disk diffusion method, according
to the 2017 French recommendations. Genetic diversity was assessed
by MultiLocus Sequence Typing (MLST). Typing data were uploaded
into BioNumerics software 7.6 to generate Minimum Spanning Trees
(MST).
Conclusion
This study showed an important genotypic diversity of PA urinary isolates. There was no epidemic clone. The MLST analysis of 2 to 5 isolates per urine sample showed that AB or UTI were rarely polyclonal (5%). In contrast, a phenotypic diversity (antibiotic susceptibility) was 
more frequently observed (17%). These results should be confirmed by the study of more isolates per urine sample and more patients.
[1]  Mulet X, Cabot G, Ocampo-Sosa AA, et al.  Biological Markers of Pseudomonas aeruginosa Epidemic High-Risk Clones. Antimicrobial Agents and Chemotherapy. 2013;57(11):5527-5535. doi:10.1128/AAC.01481-13
76%
18%
6%
Non-MDR MDR XDR
BA (in men)
UTI (in men)
BA (in women)
UTI (in women)


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Phenotypic and molecular diversity of urinary isolates of Pseudomonas aeruginosa

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