Non-coding RNAs control of phytobeneficial genes expression by pseudomonas brassicacearum

Abstract

International audienceRhizosphere microorganisms provide services to the plant, including growth promotion via hormone production, such as auxin, and protection against pathogens by producing some antibiotics such as cyanide and 2,4-diacetylphloroglucinol (DAPG). We recently showed that antifungal secondary metabolites (DAPG and cyanide), indoleacetate, exoenzymes (lipase and protease), and three different N-acyl-homoserine lactone molecules and other genes required for plant-bacteria interactions, are positively controlled by four non-coding RNAs rsmW, rsmX, rsmY and rsmZ. To shed light on phytobeneficial bacterial gene expression in situ, we investigated the expression of genes that positively control traits involved in phytostimulation and phytoprotection. For that purpose, we used constructs fusing the promoter of rsmW, rsmX, rsmY and rsmZ to fluorescent proteins reporter genes. To evaluate the expression level, fluorescent spectrosphotometry was used, and to image expression in planta, confocal laser microscopy was used. This study highlights the key role played by non-coding RNAs in plant-bacteria interaction