Sol-gel derived nanostructured cerium oxide (CeO(2)) film deposited on gold (Au) electrode has been utilized for physisorption of glucose oxidase (GOx). X-ray diffraction, atomic force microscopy, UV-visible spectroscopy, and electrochemical techniques have been used to characterize sol-gel derived CeO(2)/Au electrode and GOx/CeO(2)/Au bioelectrode. The response characteristics of the glucose bioelectrode (GOx/CeO(2)/Au) indicate linearity, detection limit and shelf-life as 50-400 mg/dL, 12.0 mu M, and 12 weeks, respectively. The value of apparent Michaelis-Menten constant (K(m)) of GOx/CeO(2)/Au bioelectrode has been found to be 13.55 mu M
