In summary, we have performed a complete characterization of LV post-infarction remodeling in a DBA/2J mouse model of MI, using parameters adapted to the particular characteristics of the model In the future, this well characterized model will be used in both investigative and pharmacological studies that require accurate quantitative monitoring of cardiac recovery after myocardial infarction

Albiasu, E. (Edurne)

Benavides, C. (Carolina)

Castaño, S. (Sara)

Corbacho, D. (David)

Iglesias-García, O. (Olalla)

Muñoz-Barrutia, A. (Arrate)

Ortiz-de-Solorzano, C. (Carlos)

Pelacho, B. (Beatriz)

Prosper, F. (Felipe)

PLoS ONE

PubMed

BackgroundThe aim of this article is to present an optimized acquisition and analysis protocol for the echocardiographic evaluation of left ventricle (LV) remodeling in a mouse model of myocardial infarction (MI).Methodology13 female DBA/2J mice underwent permanent occlusion of the left anterior descending (LAD) coronary artery leading to MI. Mice echocardiography was performed using a Vevo 770 (Visualsonics, Canada) before infarction, and 7, 14, 30, 60, 90 and 120 days after LAD ligation. LV systolic function was evaluated using different parameters, including the fractional area change (FAC%) computed in four high-temporal resolution B-mode short axis images taken at different ventricular levels, and in one parasternal long axis. Pulsed wave and tissue Doppler modes were used to evaluate the diastolic function and Tei Index for global cardiac function. The echocardiographic measurements of infarct size were validated histologically using collagen deposition labeled by Sirius red staining. All data was analyzed using Shapiro-Wilk and Student's t-tests.Principal findingsOur results reveal LV dilation resulting in marked remodeling an severe systolic dysfunction, starting seven days after MI (LV internal apical diameter, basal = 2.82±0.24, 7d = 3.49±0.42; pConclusions/significanceIn summary, we have performed a complete characterization of LV post-infarction remodeling in a DBA/2J mouse model of MI, using parameters adapted to the particular characteristics of the model In the future, this well characterized model will be used in both investigative and pharmacological studies that require accurate quantitative monitoring of cardiac recovery after myocardial infarction

Carolina Benavides-Vallve

David Corbacho

Olalla Iglesias-Garcia

Beatriz Pelacho

Edurne Albiasu

Sara Castaño

Arrate Muñoz-Barrutia

Felipe Prosper

Carlos Ortiz-de-Solorzano

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New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction.

<div><h3>Background</h3><p>The aim of this article is to present an optimized acquisition and analysis protocol for the echocardiographic evaluation of left ventricle (LV) remodeling in a mouse model of myocardial infarction (MI).</p> <h3>Methodology</h3><p>13 female DBA/2J mice underwent permanent occlusion of the left anterior descending (LAD) coronary artery leading to MI. Mice echocardiography was performed using a Vevo 770 (Visualsonics, Canada) before infarction, and 7, 14, 30, 60, 90 and 120 days after LAD ligation. LV systolic function was evaluated using different parameters, including the fractional area change (FAC%) computed in four high-temporal resolution B-mode short axis images taken at different ventricular levels, and in one parasternal long axis. Pulsed wave and tissue Doppler modes were used to evaluate the diastolic function and Tei Index for global cardiac function. The echocardiographic measurements of infarct size were validated histologically using collagen deposition labeled by Sirius red staining. All data was analyzed using Shapiro-Wilk and Student's t-tests.</p> <h3>Principal Findings</h3><p>Our results reveal LV dilation resulting in marked remodeling an severe systolic dysfunction, starting seven days after MI (LV internal apical diameter, basal = 2.82±0.24, 7d = 3.49±0.42; p<0.001. End-diastolic area, basal = 18.98±1.81, 7d = 22.04±2.11; p<0.001). A strong statistically significant negative correlation exists between the infarct size and long-axis FAC% (r = −0.946; R<sub>2</sub> = 0.90; p<0.05). Moreover, the measured Tei Index values confirmed significant post-infarction impairment of the global cardiac function (basal = 0.46±0.07, 7d = 0.55±0.08, 14 d = 0.57±0.06, 30 d = 0.54±0.06, 60 d = 0.54±0.07, 90 d = 0.57±0.08; p<0.01).</p> <h3>Conclusions/Significance</h3><p>In summary, we have performed a complete characterization of LV post-infarction remodeling in a DBA/2J mouse model of MI, using parameters adapted to the particular characteristics of the model In the future, this well characterized model will be used in both investigative and pharmacological studies that require accurate quantitative monitoring of cardiac recovery after myocardial infarction.</p> </div

Carolina Benavides-Vallve (148700)

David Corbacho (148706)

Olalla Iglesias-Garcia (148711)

Beatriz Pelacho (148716)

Edurne Albiasu (148721)

Sara Castaño (148724)

Arrate Muñoz-Barrutia (148726)

Felipe Prosper (4721076)

Carlos Ortiz-de-Solorzano (148730)

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New Strategies for Echocardiographic Evaluation of Left Ventricular Function in a Mouse Model of Long-Term Myocardial Infarction

Prosper-Cardoso, F. (Felipe)

Dadun, University of Navarra

New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction

Universidad de Navarra

Crossref

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World Health Organization (2011) The International Journal of Public Health: media centre of cardiovascular diseases, the world health report

http://dx.doi.org/10.1371/journal.pone.0041691

New strategies for echocardiographic evaluation of left ventricular function in a mouse model of long-term myocardial infarction

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Dadun, University of Navarra

Universidad de Navarra

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