Measuring intracellular metabolism has increasingly led to important insights in biomedical research. [superscript 13]C tracer analysis, although less information-rich than quantitative [superscript 13]C flux analysis that requires computational data integration, has been established as a time-efficient method to unravel relative pathway activities, qualitative changes in pathway contributions, and nutrientcontributions. Here, we review selected key issues in interpreting [superscript 13]C metabolite labeling patterns, with the goal of drawing accurate conclusions from steady state and dynamic stable isotopic tracer experiment
