Attempts were made to develop DNA
probes for M. tuberculosis. Random library of M.
tuberculosis was constructed in plasmid pGEM -4.
Selection of recombinant clones was made by hybridisation
with 32P labelled M. tuberculosis probe.
Ten recombinant clones were selected on the basis
of strong signals from the random library.
These 10 clones named pTRC1-10 were subjected to
tests for specificity and sensitivity. On this basis,
pTRC4 was chosen and this is also, useful in restriction
fragment length polymorphism (RFLP)
studies