This paper reports the preliminary findings of an investigation
using Medium V for culture of M. leprae. The medium was
prepared adhering to the procedures adopted by Veeraraghavan.
Tissues from 7 cases of lepromatous leprosy (Bacteriological
Index of 3 or more) were inoculated into Medium V and incubated
at 8–10°C for 72 hours. Quantitative estimation of leprosy
bacilli were made employing the enumeration technique of
Veeraraghavan. There was no evidence of multiplication in any
of the cultures. The findings are in conformity with those of
Kato (1983) and of Katoch and Desikan (1983)