A simple column chromatographic method for determination of ethambutol (EMB) in pharmaceutical preparations

containing EMB in combination with other anti-TB drugs is presented. The method involved extraction of EMB into an

organic solvent. followed by basification and column chromatographic separation on Amberlite CG 50 (100-200 mesh) and

elution with suitable eluants and estimation at a wavelength of 270 nm. The assay was linear from 25 to 400 μg/ml. The

relative standard deviations of intra and inter day assays were lower than 5%. Ethambutol was recovered from human urine

quantitatively and stable for a period of atleast one week in urine stored at-20°C

Bhagavathy, S

Gayathri, T N

Gurumurthy, Prema

Venkatesan, P

68-73A simple column chromatographic method
for determination of ethambutol (EMB) in pharmaceutical preparations containing
EMB in combination with other anti-TB drugs is presented. The method involved extraction
of EMB into an organic solvent, followed by basification and column
chromatographic separation on Amberlite CG 50 (100-200 mesh) and elution with suitable
eluants and estimation at a wavelength of 270 nm. The assay was linear from 25
to 400μg/ml. The relative standard deviations of intra and inter day
assays were lower than 5%. Ethambutol was recovered from human urine quantitatively
and stable for a period of atleast one week in urine stored at-20°C

Standardization of the method for estimation of ethambutol in pharmaceutical preparations and biological fluid

Abstract

Similar works

Full text

Available Versions

NOPR

NIRT Institutional Repository