The nematode cuticle is an extremely flexible and resilient exoskeleton that permits locomotion via
attachment to muscle, confers environmental protection and allows growth by molting. It is synthesised five
times, once in the embryo and subsequently at the end of each larval stage prior to molting. It is a highly
structured extra-cellular matrix (ECM), composed predominantly of cross-linked collagens, additional
insoluble proteins termed cuticlins, associated glycoproteins and lipids. The cuticle collagens are encoded by a large gene family that are subject to strict patterns of temporal regulation. Cuticle collagen biosynthesis
involves numerous co- and post-translational modification, processing, secretion and cross-linking steps that
in turn are catalysed by specific enzymes and chaperones. Mutations in individual collagen genes and their
biosynthetic pathway components can result in a range of defects from abnormal morphology (dumpy and
blister) to embryonic and larval death, confirming an essential role for this structure and highlighting its
potential as an ECM experimental model system
