Barba, Christophe

Fouque, Denis

Glorieux, Griet

Koppe, Laetitia

Picard, Cécile

Soulage, Christophe,

Soulage, Christophe

Picard, Cecile

Ghent University Academic Bibliography

P0922 A LOW AROMATIC AMINO-ACID DIET IMPROVES RENALFUNCTION AND PREVENTS KIDNEY FIBROSIS IN MICE WITHCHRONIC KIDNEY DISEASEChristophe Barba1,2, Christophe Soulage2, Griet Glorieux3, Cecile PICARD4,Denis Fouque1,2, Laetitia Koppe1,21Hospices Civils de Lyon, Centre Hospitalier Lyon-Sud, Department of Nephrology,Pierre-Bénite, France, 2Univ. Lyon, CarMeN lab, INSA-Lyon, INSERM U1060, INRA,Université Claude Bernard Lyon 1, VILLEURBANNE, France, 3Ghent University Hospital,Nephrology Section, Department of Internal Medicine, Ghent, Belgium and 4HospicesCivils de Lyon, Groupement Hospitalier Edouard Herriot, Department of Pathology,Bron, FranceBackground and Aims: Despite decades of use of low protein diets (LPD) in the man-agement of chronic kidney disease (CKD), the mechanisms through which it delays theprogression to end-stage renal disease (ESRD) remain controversial. A reduced pro-duction of uremic toxins could contribute to the benefits of the LPD. Aromatic amino-acids are precursors of major uremic toxins such as p-cresyl sulfate (PCS), indoxyl sul-fate (IS), indole-3-acetic acid or phenol. We investigated the hypothesis that a low aro-matic amino acid diet (LAA, namely low intake of tyrosine, tryptophan andphenylalanine) while being normoproteic, could be as effective as a LPD, through thespecific diminution of uremic toxins production.Method: Renal failure was chemically induced in mice with a diet containing 0.25% (w/w) of adenine. Thereafter, they received 3 different diets for 6 weeks: normoproteic diet(NPD: 14.7% proteins, aromatics 0.019%), LPD (5% proteins, aromatics 0.007%) andLAA (14% proteins, aromatics 0.007%).Results: LAA and LPD had no significant effect on body weight. Plasma creatinine wassignificantly lower in LPD and LAA groups compared to NPD group (72 6 4 and 73 64 mmol/L vs 127 6 6 mmol/L, p<0.0001), as well as proteinuria (1.3 6 0.3 and 1.2 6 0.1mg/24h vs 3.8 6 0.9 mg/24h, p<0.05). Kidney fibrosis was more severe in NPD groupvs LPD and LAA groups (17 6 1% vs 10 6 1 % and 13 6 2 %, p<0.0001 and p=0.01).Kidney inflammation was also reduced with LPD and LAA. Free PCS and IS were lowerin LPD and LAA groups compared to NPD group.Conclusion: These results suggest that LAA confers similar benefits as compared withthose of LPD to delay the progression of CKD through reduction of uremic toxins pro-duction, with lower risk of malnutrition.Renal function and urinary protein excretion in control and CKD miceSerum creatinine (A), blood urea nitrogen (B) and urinary proteins (C) in control andCKD mice fed with normoproteic diet (NPD), low protein diet (LPD) or low aromaticamino-acid diet (LAA). Data are expressed as mean 6 SEM for n = 5-11 animals ineach group.*p< 0.05, **p< 0.01, ***p< 0.001 vs CKD-NPD; (two-way ANOVA and Dunnettpost hoc test).Figure:Abstracts Nephrology Dialysis Transplantationiii1248 | AbstractsDownloaded from https://academic.oup.com/ndt/article/35/Supplement_3/gfaa142.P0922/5852407 by Ghent University user on 25 January 2021

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A low aromatic amino-acid diet improves renal function and prevents kidney fibrosis in mice with chronic kidney disease

Meeting Abstract: P0922International audienc

Soulage, Christophe, O.

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https://biblio.ugent.be/publication/8689831/file/8689832

A low aromatic amino-acid diet improves renal function and prevents kidney fibrosis in mice with chronic kidney disease

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