Dan Li

Jing Long

Ling Qin

Nan Wang

Qiufeng Zhou

Xinluan Wang

Journal of Orthopaedic Translation

Zhou, Qiufeng

Li, Dan

Long, Jing

Wang, Xinluan

Wang, Nan

Qin, Ling

Elsevier - Publisher Connector 

miRNA expression profiles during adipogenic and osteogenic differentiation of mouse BMSCs 

220 Abstractscartilage in the late stage, bony spur forms in RA. These symptoms are very
important in RA pathological development.
Methods: In this study, CIA was used as an animal model to elucidate further
the pathological process of bony spur. The destruction of joints in the CIA
model was observed by radiology and histology.
Results: In the radiological observation, bony spur formed in the knee and
foot joint, which worsened as the disease progressed. Meanwhile, fusion
and damage of articular cartilage was observed, and many osteoclasts
were found in the histological sections.
Conclusion: Based on previous research on the CIA model and related inves-
tigations, the bony spur may have another main pathological process in the
later stages of RA.
IBDW2014-00088-F0020
miRNA EXPRESSION PROFILES DURING ADIPOGENIC AND OSTEOGENIC
DIFFERENTIATION OF MOUSE BMSCs
Qiufeng Zhou a,b, Dan Li a, Jing Long a,b, Xinluan Wang a, Nan Wang a,
Ling Qin a,c
aTranslational Medicine Research and Development Center, Shenzhen
Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen
518055, China
bInstitute of Biotechnology, Guilin Medical University, Guilin 541004, China
cDepartment of Orthopaedics and Traumatology, The Chinese University of
Hong Kong
Objective: Identification of miRNA expression profiles during adipogenic and
osteogenic differentiation of mouse bone marrow mesenchymal stem cells
(BMSCs).
Materials and methods: BMSCs were isolated from the femurs and tibias of
4- to 6-week-old male C57BL/6 mice as previously described, and cultured in
a-MEM supplemented with 10% FBS. Then BMSCs were identified by in vitro
multi-lineage differentiation assays, including adipogenesis, osteogenesis
and chondrogenesis. Subsequently, the cells were cultured in adipogenesis
differentiation medium for 6 days and in osteogenesis differentiation me-
dium for 10 days, respectively. Uninduced cells were included as control.
miRNA profiles were analyzed using Agilent Mouse miRNA microarray slide
(8 X 60K, Part number G4872A). Hierarchical clustering was performed
with Multi-experiment Viewer (MeV) software. A selected subset of miRNAs
changed more than 1.8-fold was selected for further real-time PCR analysis.
Results: The miRNA microarray analysis showed that 66 miRNAs were differ-
entially expressed during adipogenic or osteogenic differentiation of mouse
BMSCs. Real-time PCR analysis showed that, compared with the control, the
expression level of miR-218-5p was increased 10-fold and 2.8-fold after adi-
pogenic induction for 6 days and osteogenic induction for 10 days, respec-
tively. Within the first three days of induction, miR-218-5p was increased
5.9-fold during adipogenic differentiation, yet without significant difference
during osteogenic differentiation. The expression levels of miR-146a-5p and
miR-223-3p were decreased 10.8-fold and 6.6-fold, respectively, during
osteogenic differentiation.
Conclusions: miR-218-5p was increased during both adipogenic and osteo-
genic differentiation, with a significant predominance in adipogenic differ-
entiation. In addition, miR-146a-5p and miR-223-3p were decreased during
osteogenic differentiation. An effort will be made to understand their roles
and mechanisms.
Acknowledgements
This work was supported by the grants from NSFC (No. 81200650 and
81302782).
IBDW2014-00089-F0021
ABNORMAL BONE MICRO-ARCHITECTURE AND ROD-PLATE
CONFIGURATION IN OSTEOPENIC ADOLESCENT IDIOPATHIC SCOLIOSIS
(AIS)
Ka Yee Cheuk a, Ivy J. Zhang a, ZhiWei Wang a, Vivian W. Y. Hung a,b, Bobby
K. W. Ng a, T. P. Lam a, Arthur F. T. Mak c, Jack C. Y. Cheng a
aDepartment of Orthopaedics and Traumatology, The Chinese University of
Hong Kong
bBone Quality and Health Assessment Centre, Department of Orthopaedics
and Traumatology, The Chinese University of Hong Kong, Shatin, Hong Kong
cDepartment of Mechanical and Automation Engineering, The Chinese
University of Hong Kong, Shatin, Hong Kong
Objectives: Multiple studies have documented the presence of systemic
osteopenia in AIS. Osteopenia was associated with severe curves and was re-
ported to be one of the significant prognostic factors for curve progression inAIS. This study aimed to evaluate bone quality and bone strength parameters
including rod-plate configuration and finite element analysis (FEA) with in
vivo High-Resolution Peripheral Quantitative Computed Tomography (HR-
pQCT) and to investigate their relationship with osteopenia in AIS Vs normal
controls.
Material and Methods: 101AIS and 105 controls between 11-14 years old
were recruited. Areal bone mineral density (aBMD) of bilateral femoral
necks was measured with Dual Energy X-ray Absorptiometry (DXA). Subjects
were classified into the osteopenic (Z-score<orZ-1) and non-osteopenic (Z-
score>-1) group. Bone Morphometry, volumetric bone mineral density
(vBMD) and Trabecular Bone Micro-architecture were measured using HR-
pQCT. Structural Model Index (SMI) quantifying the trabecular rod/plate
configuration (a higher index indicating more rod-like configuration) and
FEA in terms of Stiffness, Failure Load and Apparent Modulus were calcu-
lated with a standard algorithm.
Results: In the AIS group, osteopenic subjects showed higher SMI, lower
Stiffness, lower Failure Load and lower Apparent Modulus when compared
with non-osteopenic subjects (% difference Z 15.5%, -24.5%, -23.1% &
-20.5% respectively, all with p<0.001). Similar differences in FEA profiles
were noted between osteopenic and non-osteopenic subjects in the control
group. In contrast, no significant difference in SMI was found between osteo-
penic and non-osteopenic controls. When all osteopenic subjects were
considered, osteopenic AIS subjects had higher SMI when compared with
osteopenic controls (% difference Z 9.1%, pZ0.012).
Conclusions: This study showed that osteopenia was associated with lower
bone strength and a specific pattern of SMI indicating preponderance of
rod-like configuration in AIS subjects. Notably the association of higher SMI
with osteopenia was seen in AIS but not in normal controls, thus providing
strong evidences that osteopenia in AIS was different from osteopenia in
non-AIS controls. Further investigations exploring the underlying biochem-
ical and biomechanical mechanisms that bring about these specific endophe-
notypes are warranted for gaining further understanding of the
etiopathogenesis of AIS.
This study was supported by Research Grants Council of the Hong Kong
S.A.R., China (Project no: 468809 and 468411).
IBDW2014-00090-F0022
EVALUATING BONE STRENGTH WITH FINITE ELEMENT ANALYSIS FOR
ADOLESCENT IDIOPATHIC SCOLIOSIS (AIS): A CASE-CONTROL STUDY
WITH HR-pQCT
Ka Yee Cheuk a, Lyn L. N. Wong a, Alec Hung a, Arthur F. T. Mak b,
K. M. Lee c,d, Bobby K. W. Ng a, T. P. Lam a, Jack C. Y. Cheng a,d
aDepartment of Orthopaedics and Traumatology, The Chinese University of
Hong Kong, Shatin, Hong Kong
bDepartment of Mechanical & Automation Engineering, The Chinese
University of Hong Kong, Shatin, Hong Kong
cLee Hysan Clinical Research Laboratory, The Chinese University of Hong
Kong
dJoint Scoliosis Research Center of the Chinese University of Hong Kong and
Nanjing University, Hong Kong, China
Objectives: Although Adolescent Idiopathic Scoliosis (AIS) was associated
with low bone mass, reports on bone mechanical properties in AIS are
sparse. The objective of this study is to evaluate bone mechanical properties
with finite element analysis (FEA) using in-vivo High-Resolution Peripheral
Quantitative Computed Tomography (HR-pQCT) in AIS and compare that
with normal controls.
Material and Methods: 97 AIS girls and 99 female controls between 11-14
years old were recruited. Dietary calcium intake and physical activity level
were assessed with a standard Food Frequency Questionnaire and the Modi-
fied Baecke Questionnaire respectively. With HR-pQCT, an established model
on morphology and micro-structure of the non-dominant distal radius was
generated for FEA in terms of Stiffness, Failure Load and Apparent Modulus.
Multivariate linear regression analysis was used to investigate the difference
between AIS and controls after adjusting for age in Model 1 and for age, cal-
cium intake and physical activity level in Model 2.
Results: 2-tailed Student’s t-test showed AIS subjects had lower Stiffness,
lower Failure Load and lower Apparent Modulus when compared with normal
controls (% difference Z -6.81%, -7.10% & -8.10% respectively, all with
p<0.05). AIS girls had lower Failure Load (BZ-136.0, pZ0.04) and lower
Apparent Modulus (BZ-146.2, pZ0.021) in Model 1 with adjustment for
age. In Model 2, difference in Apparent Modulus remained statistically signif-
icant with AIS being associated with lower Apparent Modulus after adjust-
ment for age, calcium intake and physical activity level (BZ-137.1,
pZ0.037).


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miRNA expression profiles during adipogenic and osteogenic differentiation of mouse BMSCs

miRNA expression profiles during adipogenic and osteogenic differentiation of mouse BMSCs

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