Qian, Xiaoxian

Yu, Shujie

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J O U R N A L O F T H E A M E R I C A N C O L L E G E O F C A R D I O L O G Y , V O L . 6 6 , N O . 1 6 , S U P P L S , 2 0 1 5 C103RESULTS (1) compared with the blank control group, LPS group and
0 m mol/L rosiglitazone group on 6, 12, 24h, HMGB1 secretion
increased signiﬁcantly (P<0.01). Compared with the LPS group, 5 m
mol/Lrosiglitazone group HMGB1 decreased signiﬁcantly on 6, 12, 24h
(P<0.01). Compared with 6h, LPS group and 0 m mol/L rosiglitazone
group HMGB1 secretion increased signiﬁcantly on 12, 24h; 5 m mol/L
rosiglitazone group decreased signiﬁcantly on 12, 24h (P<0.05,
P<0.01). (2) HMGB1 secretion of 5, 10, 15m mol/L rosiglitazone group
signiﬁcantly reduced on 24h compared with LPS Group. [(165.77 
20.29) ng/ml, (136.63  15.90) ng/ml, (112.25  12.23) ng/ml VS (338.74
 18.22) ng/ml, P<0.05]. HMGB1 secretion of 24h in 10, 15 m mol/L
rosiglitazone group is decreased signiﬁcantly compared with 5 mmol/L
rosiglitazone group (P<0.05, P<0.01).
CONCLUSIONS 1mg/L LPS could obviously stimulate the production
of HMGB1 on HUVECs. And it was remarkably inhibited by Rosiglita-
zone in a concentration and time-dependent manner. The starts up of
HMGB1 expression induced by LPS may thereby be one of the mech-
anism participated in the pathogenesis of atherosclerosis.
GW26-e2389
Ginsenoside Rb1 Attenuates Oxidative Stress and Inﬂammation in Aged
Mice
Shujie Yu,1 Xiaoxian Qian1,2
1Department of Cardiology, The Third Afﬁliated Hospital of Sun Yat-sen
University, Guangzhou, Guangdong 510630, China; 2Institute
Integrated Traditional Chinese and Western Medicine, Sun Yat-sen
University, Guangzhou, Guangdong 510630, China
OBJECTIVES Our previous studies have shown that Ginsenoside Rb1
(Rb1) prevents endothelial cells from senescence through modulating
redox status. Oxidative stress and inﬂammation are involved in aging
and age-related phenomenon. This study was performed to investi-
gate the role of Rb1 in aged mice.
METHODS Female C57/B6 mice were used in the study, which were
divided into four groups including control mice (2-3 months), aged
mice (22-24 months), Rb1-treated mice (20 mg/kg, 22-24 months). The
SOD1 activity and levels of NO and MDA were detected using the
corresponding commercially available kits. Levels of ICAM-1 and MCP-
1 were examined by ELISA.
RESULTS The oxidative stress was increased in aged mice as shown
by decreased SOD1 activity and increased MDA production compared
to the young control mice, while administration of Rb1 for 6 weeks
was able to reverse the oxidative status signiﬁcantly. Meanwhile,
serum levels of ICAM-1 and MCP-1 were increased in aged mice and
were down-regulated after Rb1 treatment. Chronic treatment of Rb1
also partially prevented the loss of body weight in aged mice.
CONCLUSIONS Our study showed that chronic administration of Rb1
was able to attenuate both oxidative stress and inﬂammation in aged
mice. The study may indicate Rb1’s potential effect in age-related
cardiovascular diseases.
GW26-e3579
Feasible and Controllable Creation of Tricuspid Regurgitation Beagle Dog
Model from Tricuspid Leaﬂet Resection in open beating heart
Xujing Xie,1 Huanlei Huang2
1The Third Afﬁliated Hospital of Sun Yat-sen University; 2Guangdong
Provincial Cardiovascular Institute, Guangdong General Hospital,
Guangdong Academy of Medical Sciences
OBJECTIVES To create a controllable and clinical compatible
tricuspid regurgitation animal model for studying right heart remod-
eling caused by TR.
METHODS Fourteen juvenile male Beagle dogs were randomly
divided into intervention group (n¼11) and sham-operated control
group (n¼3). Intervention group underwent thoracotomy and right
atrium (RA) incision following occlusion of superior and inferior vena
cava, anterior leaﬂet of tricuspid valve was resected in 8 dogs, both
anterior and posterior leaﬂets were resected in 3. Afterward, RA and
chest was closed. Animals were planned to be raised for 3 years.
Control group underwent the same procedure exception of leaﬂets
resection. One intervention dog and one control dog underwent
euthanasia and autopsy at 12m after surgery.
RESULTS All dogs survived the surgery and during 1-year observa-
tion. Severe TR immediately occurred after surgery in dogs with
anterior leaﬂets resection, and extremely severe TR in those with two
leaﬂets resection. Duration from thoracotomy to chest closure was
30w40min, and the mean time of superior and inferior vena cavaocclusion was 8710s. CVP increased from baseline 61.2mmHg to
131.7mmHg (p<0.01). In intervention group, RA area, tricuspid
annular diameter and RV index of myocardial performance increased,
percent fractional area change of right ventricle and tricuspid annular
plane systolic excursion (TAPSE) decreased, and autopsy presented
edema, ascites, and cirrhosis morphology.
CONCLUSIONS The protocols of creation of TR were controllable,
reproducible, and with high successful and survival rate. Animal
model of these surgeries provide opportunities to mechanistically
investigate right heart remodeling.
GW26-e5356
Structure-activity Relationships of Flavonoids in Plants of Ampelopsis
Grossedentat on Inhibiting hKv1.5 Channels
Xianhong Ou,1 Xiaohong Bin,1 Miaoling Li,1 Yefei Yuan,2 Yong Ye,3
Rui Liu,1 Xinrong Fan,1 Luzhen Wang,1 Yan Yang,1 Xiaorong Zeng1
1Ministry of Education of China, and the Institute of Cardiovascular
Research, Sichuan Medical University, Luzhou 646000, Sichuan,
China; 2College of Pharmacy, Sichuan Medical University, Luzhou,
Sichuan, China; 3College of pharmacy, Guangxi Medical University,
Nanning, Guangxi, China
OBJECTIVES To study the structure-activity relationships of Flavo-
noids in Plants of Ampelopsis Grossedentat on Inhibiting hKv 1.5
Channels.
METHODS To study the structure-activity relationships of Flavonoids
in Plants of Ampelopsis Grossedentat on inhibiting hKv 1.5 Channels.
RESULTS Both Myr and DMY could inhibit hKv1.5 Channels. The
current density of IKur was reduced from 215.02  40.59 (pA/pF) to
77.7217.94 (pA/pF) (n¼5, P¼0.011 <0.05, VS Control), 116.5914.30 to
57.239.57 pA / pF (n ¼ 14, P < 0.001) respectively by treatment with
10 mMMyr or DMY for 20 min. Moreover, they inhibited Ikur in HEK 293
cells in a manner that dose-, time- and frequency-dependence, and
inhibited hKv1.5 protein in a dose-dependent manner.
CONCLUSIONS The Inhibition hKv 1.5 Channels activity of Flavonoids
in Plants of Ampelopsis Grossedentat is related to the nucleus of ﬂa-
vonoids, but may by not related to 2, 3-ﬂavonone.
GW26-e1056
Qishenyiqi attenuates myocardial ﬁbrosis in rats by inhibiting RAAS-
mediated arachidonic acid inﬂammation
Linghui Lu,1 Jing Wang,2 Yan Wu,3 Jing Han,3 Yong Wang,1 Chun Li,4
Wei Wang1
1Basic Medical College, Beijing University of Chinese Medicine, Beijing,
China; 2School of Chinese Materia Medica, Beijing University of
Chinese Medicine, Beijing, China; 3Center of Scientiﬁc Experiment,
Beijing University of Chinese Medicine, Beijing, China; 4Modern
Research Center for Traditional Chinese Medicine, Beijing University of
Chinese Medicine, Beijing, China
OBJECTIVES Myocardial ﬁbrosis is a common pathological progress
of cardiovascular diseases and treatment of ﬁbrosis may improve
clinical efﬁcacy and prognosis of heart failure (HF) patients. Previous
study has shown that Qishenyiqi (QSYQ) has deﬁnite therapeutic ef-
fects on reversing cardiac ﬁbrosis, while its underlying mechanism
remains unrevealed. The present study aims to demonstrate that
QSYQ treatment can exert anti-ﬁbrosis effects in HF rats mainly by
inhibiting Renin-angiotensin-aldosterone System (RAAS) and arach-
idonic acid (AA) metabolic pathway.
METHODS Sprague-Dawley (SD) rats were randomly divided into 4
groups: sham group, model group (left anterior descending coronary
artery ligation), captopril group (LAD ligation and treated with
captopril) and QSYQ group (LAD ligation and treated with QSYQ). 28
days after surgery, 2D echocardiography was adopted to detect the
heart function. HE and Masson’s trichrome staining were performed
to assess the degree of myocardial ﬁbrosis. Collagen (I, III) contents
and matrix metalloproteinases (MMP2, MMP9) levels were assayed by
immunohistochemical method. Western-blot was used to detect
expression of critical proteins in RAAS (AT1, AT2) or AA metabolic
pathway (PLA2 and COX1/COX2), and their potential signal-trans-
ducing proteins (JAK1/STAT3, NFkb and Akt).
RESULTS At 28 days after surgery, echocardiography showed that
LVEDd and LVEDs in the model group increased signiﬁcantly, indi-
cating the induction of ventricular remodeling. HE and Masson’s tri-
chrome stain results also demonstrated pathological changes of
ﬁbrosis. Expressions of ﬁbrotic markers, such as Collagen (I, III),
MMP2 and MMP9, were elevated in model group. Level of AT1 in RAAS


GW26-e2389 Ginsenoside Rb1 Attenuates Oxidative Stress and Inflammation in Aged Mice 

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GW26-e2389 Ginsenoside Rb1 Attenuates Oxidative Stress and Inflammation in Aged Mice

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