Chao, L.-L.

Shih, C.-M.

International Journal of Infectious Diseases

Elsevier - Publisher Connector 

Molecular evidence of genetic diversity of Borrelia burgdorferi sensu lato detected in Ixodes granulatus ticks removed from rodents in Taiwan 

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Conclusion: All the fourteen strains of Ureaplasma iso-
lated from cows with various reproductive disorders show
multiple drug resistance against tested antibiotics.
doi:10.1016/j.ijid.2010.02.1832158 14
Results: Of the 94 dogs analyzed, 51 were male and
3 females. The mean age for both males and females
as 3.6 years (range 4 months — 15 years).Serological and
arasitological tests revealed that 12 dogs (12.8%) were
rypanosome infected (Table 1). Nine dogs (9.6%) had anti-
odies against T. cruzi. Trypanosomes were isolated in three
5.3%) hemoculture samples. Molecular analysis showed that
solated trypanosomes were T. rangeli. None of these T.
angeli positive dogs had detectable antibodies against T.
ruzi. Four infected dogs belong to people with Chagas dis-
ase diagnosis.
Conclusion: In conclusion our data demonstrate that dogs
re frequently infected with Trypanosomes in this area o
anama with a prevalence similar to the one observed in the
uman population. This study improves our understanding
f the epidemiology and control of Chagas disease in rural
reas of central Panama.
oi:10.1016/j.ijid.2010.02.1830
4.009
olecular evidence of genetic diversity of Borrelia
urgdorferi sensu lato detected in Ixodes granulatus ticks
emoved from rodents in Taiwan
.-L. Chao, C.-M. Shih ∗
National Defense Medical Center, Taipei, Taiwan, R.O.C
Background: Genetic diversity of Borrelia spirochetes in
xodes granulatus ticks of Taiwan remains unknown and
eeds further identiﬁed.
Methods: A general survey was conducted to collect
. granulatus ticks removed from trapped rodents in Tai-
an. Total genomic DNA was extracted from individual tick
pecimen by using DNeasy Blood & Tissue Kit (Qiagen).
enetic identities of Borrelia spirochetes detected in I.
ranulatus ticks were determined by analyzing the gene
equences ampliﬁed by a nested polymerase chain reaction
PCR) assay based on the 5S-23S intergenic spacer amplicon
ene of B. burgdorferi sensu lato. Phylogenetic relation-
hips of these detected spirochetes were further analyzed by
eighbour-joining (NJ) compared with maximum parsimony
MP) methods.
Results: A total of 261 I. granulatus ticks (156 adults
nd 105 nymphs) were tested by nested-PCR assay and
orrelia spirochetes were detected in 80 adults and 52
ymphs with an infection rate of 51.3% and 49.5%, respec-
ively. Phylogenetic analysis reveals that all these detected
pirochetes constitute two major separate clades distincted
rom other Borrelia genospecies in both NJ and MP meth-
ds. Within the clades, 10 strains of Borrelia spirochetes
etected in I. granulatus ticks were closely related to
he genospecies of B. burgdorferi sensu stricto and 15
trains of detected spirochetes were closely related to
. valaisiana.
Conclusion: Our results demonstrate the genetic diver-
ity of B. burgdorferi sensu lato spirochetes detected in I.
ranulatus ticks collected in Taiwan. The genetic identities
f these detected spirochetes were clariﬁed by analyzing
equence homology of 5S-23S intergenic spacer ampli-
on gene. Further investigations on Borrelia spirochetes
etected in variant tick species and reservoir hosts wouldternational Congress on Infectious Diseases (ICID) Abstracts
eneﬁcial to the better understanding of genetic hetero-
eneity of Borrelia spirochetes in Taiwan.
oi:10.1016/j.ijid.2010.02.1831
4.010
ccurrence of Ureaplasma diversum in cows with various
eproductive disorders
. Chandra1, Y. Singh2,∗, D. Nand Garg3
CCS Haryana Agricultural University, Hisar, Hisar, Haryana,
ndia
CCS Haryana Agricultural University, Hisar, Hisar, India
CCS Haryana Agricultural University, Hisar, Hisar, Haryana,
ndia
Background: Ureaplasma diversum, a bovine species was
rst isolated by Taylor Robinson and co-workers in 1967 from
attle. The genital ureaplasmosis in cows occurs in vari-
us clinical forms viz. urethritis, endometritis, salpingitis,
ranular vulvoveginitis, abortion and neonatal calf mortality
eading to temporary or permanent infertility.
Methods: During present study, a mycoplasmological
xamination of cervico-vaginal swabs/vaginal discharges
rom 136 cows including 86 with various reproductive dis-
rders (22 anoestrus, 25 repeat breeder, 6 cervicitis, 17
etritis, 16 abortion/still-birth) and 50 apparently healthy
ows was conducted.The U-9B liquid medium was used for
solation of Ureaplasmas. In-vitro antibiotic sensitivity of
reaplasmal isolates against ten selected antibiotics was
erformed at ﬁrst stage of their cultivation in U-9B colour
est liquid medium.
Results: The mycoplasmological examination of cervico-
aginal swabs/vaginal discharges from 136 cows including
6 with various reproductive disorders and 50 apparently
ealthy cows resulted in isolation of 14Ureaplasma species
long with 11 Mycoplasma and 8 Acholeplasma.The inci-
ence of mollicutes was was found higher in genitally
iseased cows 929.07%) as compared to apparently healthy
ows (16%). The prevalence of Ureaplasma diversum was
ore in repeat breed cow (20%) than anoestrus (9.8%), cer-
icitis and metritis (4.9%). However, noUreaplasma strain
as isolated from abortion cases. The concentration of
ine strains of Ureaplasma isolated from cows with vari-
us reproductive disorders and 5 from apparently healthy
anged between 5x102 to 5x104ccu/ml. All the test strains of
reaplasma, were found sensitive for lincospectin and resis-
ant to ampicillin. However, variable resistance was shawn
y 6 isolates to tetracycline, 4 isolates to enroﬂoxacin,
piramycin and chloramphenicol, 3 isolates to tylosin and
rythromycin and one isolate to tiamutin and sparﬂoxacin.


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Molecular evidence of genetic diversity of Borrelia burgdorferi sensu lato detected in Ixodes granulatus ticks removed from rodents in Taiwan

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