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Bone marrow stromal cells stimulate an angiogenic program that requires endothelial MT1‐MMP
Authors
Au
Brooks
+48 more
Caplan
Carmeliet
Clark
Crisan
Egeblad
Farrell
Ferrari
Filippov
Fuchs
Ghajar
Ghajar
Ghajar
Ghajar
Gocheva
Gocheva
Grigoriadis
Hanemaaijer
Hanjaya-Putra
Hauner
Heissig
Hellstrom
Hildenbrand
Hotary
Kachgal
Kroon
Lehti
Lu
Lutolf
Moon
Mori
Morikawa
Nakatsu
Ozawa
Phelps
Raeber
Rehman
Sato
Seliktar
Siedentop
Sounni
Toth
Turk
Uttamchandani
van Hinsbergh
Webster
Yana
Yu
Zandonella
Publication date
1 November 2012
Publisher
'Wiley'
Doi
Cite
View
on
PubMed
Abstract
Bone marrow‐derived stromal/stem cells (BMSCs) have recently been characterized as mediators of tissue regeneration after injury. In addition to preventing fibrosis at the wound site, BMSCs elicit an angiogenic response within the fibrin matrix. The mechanistic interactions between BMSCs and invading endothelial cells (ECs) during this process are not fully understood. Using a three‐dimensional, fibrin‐based angiogenesis model, we sought to investigate the proteolytic mechanisms by which BMSCs promote vessel morphogenesis. We find that BMSC‐mediated vessel formation depends on the proteolytic ability of membrane type 1‐matrix metalloproteinase (MT1‐MMP). Knockdown of the protease results in a small network of vessels with enlarged lumens. Contrastingly, vessel morphogenesis is unaffected by the knockdown of MMP‐2 and MMP‐9. Furthermore, we find that BMSC‐mediated vessel morphogenesis in vivo follows mechanisms similar to what we observe in vitro. Subcutaneous, cellular fibrin implants in C.B‐17/SCID mice form aberrant vasculature when MMPs are inhibited with a broad‐spectrum chemical inhibitor, and a very minimal amount of vessels when MT1‐MMP proteolytic activity is interrupted in ECs. Other studies have debated the necessity of MT1‐MMP in the context of vessel invasion in fibrin, but this study clearly demonstrates its requirement in BMSC‐mediated angiogenesis. J. Cell. Physiol. 227: 3546–3555, 2012. © 2012 Wiley Periodicals, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/92409/1/24056_ftp.pd
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Last time updated on 10/08/2012
Crossref
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info:doi/10.1002%2Fjcp.24056
Last time updated on 01/04/2019