Solid/liquid extraction as key step for quality assessment of commercial cranberry products usingHPTLC-densitometry

Abstract

Poster Session F : Innovative Approaches in polyphenol researchIncreasing number of dietary supplement containing cranberry polyphenols are commercialized every year. The composition of cranberry ingredients varies from whole fruit powder to cranberry extract or mixture with blueberry.In order to analyze the quality of cranberry based commercial products, researchers proceed to quality control based on BL-DMAC analysis after one step extraction. In our previous work, we demonstrated that BL-DMAC analysis is not sufficient alone to assess the quality of cranberry ingredients. BL-DMAC as quality control couple to High Performance Thin Layer Chromatography -densitometry bring complementary information, especially rate of epicatechin, PAC-A2and PAC-B2.In this work, different extraction solvents were screened for extraction of PACs from a range of commercial dietary supplements. Thus, solid / liquid extraction step was repeated until total extraction of polyphenols was achieved, i.e no coloration of the residue in contact with DMAC reagent. If the solvent nature was found to have a minor impact, the number of required extraction steps varied significantly between commercial products. This step was then demonstrated to be a key factor to control for meaningful comparison between various food supplements.Quality assessment of cranberry extracts was then conducted using both BL-DMAC and HPTLC-densitometry protocols, in order to obtain global PACs content and metabolic profile. A large diversity of PACs global content and profile was observed[br/] This work highlighted the need of new standardization protocols to control and asses the quality of cranberry ingredients, to guarantee biological effect