Choi, Hyo Seon

Han, Sle Gi Lo

Jo, Uk Hyun

Jung, Jun Seok

Kim, Jun Suk

Kim, Yeul Hong

Lee, Hyo Jung

Lee, Jae Won

Sung, Jae Sook

Yoo, Won Beak

Elsevier - Publisher Connector 

P2-014: Single nucleotide polymorphisms of Pim-1 in Korean lung cancer patients 

Copyright © 2007 by the International Association for the Study of Lung Cancer S487
Journal of Thoracic Oncology • Volume 2, Number 8, Supplement 4, August 2007  12th World Conference on Lung Cancer
tion was achieved less often in women than in men (p=0.004). In 
men,the expression of EGFR,VEGF,p53,P21,CerbB2 of NSCLC 
was 47.1%,36.0%, 36.8%,49.3% and 30.1% respectively. In women, 
the expression of EGFR, VEGF, p53,P21, CerbB2 of NSCLC was 
42.4%,29.6%,21.6%,26.4% and 48.8% respectively. Female gender 
signiﬁcantly increased the risk of high expression of CerbB2, RR: 
2.208, 95%CI: 1.330-3.667, p=0.002, and decreased the risk of high 
expression of P21, RR: 0.372, 95%CI: 0.159--0.870 p=0.023. The high 
expression of EGFR and VEGF may indicated the poor prognosis. The 
expression of p53 was signiﬁcantly related to smoking.
Conclusions: Women with lung cancer were more peripheral type 
and smoked less intensively but had more passive smoking. Over-rep-
resentation of adenocarcinoma and smaller lesions was observed in 
the women. Women with lung cancer had a better lung function and 
expression of CerbB2 in NSCLC related to women but expression of 
P21 has been veriﬁed associated with smoking correlated with men. 
Our study suggested the interaction between environmental and genetic 
factors is important.
P2-014 BSTB: Cancer Genetics Posters, Tue, Sept 4 
Single nucleotide polymorphisms of Pim-1 in Korean lung cancer 
patients
Jung, Jun Seok; Han, Sle Gi Lo; Jo, Uk Hyun; Yoo, Won Beak; Choi, 
Hyo Seon; Sung, Jae Sook; Lee, Hyo Jung; Lee, Jae Won; Kim, Jun 
Suk; Kim, Yeul Hong 
Korea University, Seoul, Korea
The Pim-1 is a proto-oncogene and a member of the serine/threonine 
protein kinses family. The expression of Pim-1 protein is mediated 
through activation of JAK/STATs. Pim-1 is associated with multiple 
cellular functions such as proliferation, differentiation, apoptosis and 
tumorigenesis. In specially, pim-1 is known as a important player in 
the process of malignant transformation. We therefore, investigated 
whether SNPs of Pim-1 are associated with Korean lung cancer carci-
nogenesis. 
We analyzed 4 SNP points (-1969 G/A, -766 T/C, 2760 T/A and 4115 
C/A) of Pim-1 gene by using the genomic DNA from 312 Korea lung 
cancer patients and 257 healthy controls. Genotyping was performed 
by the SNP-IT, SNapShot assay and Restriction Fragment Length Poly-
morphism (RFLP). The allele frequencies of each SNP between lung 
cancer patients and controls were estimated by the chi-square tests and 
odds rations (OR) with 95% conﬁdence interval (95% CI). We explored 
the association between SNP points and clinicopathologic parameters 
of the lung cancer patients.
-1969 locus were associated with lung cancer risk. Also, in case of 
-1969 snp point, subgroup analysis of clinicopathologic parameters in 
cancer group showed signiﬁcant association with Sex, Age, Smoking, 
Drinking, and Cell Type. In CC genotype of the -766 locus, risk of lung 
cancer was associated with old age(OR=0.483; 95% CI, 0.246-0.950). 
Also, In AT genotype of the 2760 locus, Risk of lung cancer was 
increased in none smoking(OR=1.818; 95% CI, 1.018-3.223) group 
compared with smoking group in lung cancer patients.
We conﬁrmed a relation between the Pim-1 polymorphisms and signiﬁ-
cantly increased risk in certain subgroup of lung cancer patients.
This study was supported by a Grant of the Korea Health 21 R&D proj-
ect, the Ministry of Health & Welfare, Republic of Korea (A010250)
P2-015 BSTB: Cancer Genetics Posters, Tue, Sept 4 
Frequent DNA copy number gains in 2p in lung cancer
Kettunen, Eeva1 Aavikko, Mervi1 Nymark, Penny1 Ruosaari, Salla1,2 
Wikman, Harriet3 Kuosma, Eeva1 Anttila, Sisko1 
1 Finnish Institute of Occupational Health, Helsinki, Finland 2 Helsinki 
University of Technology, Helsinki, Finland 3 Department of Tumor 
Biology, University Medical Center Hamburg-Eppendorf, Hamburg, 
Germany 
Background: Lung tumours often display very complex karyotypes 
(reviewed in Mitelman database). There can either be additional DNA 
sequences and extra chromosomes or deletions of sequences or even 
missing whole chromosomes in otherwise polyploidic genomes. Previ-
ous molecular karyotyping experiments have shown that in chromo-
some 2 there are frequently gained sequences in lung cancer. We have 
studied DNA copy numbers in the centromeric region of chromosome 2 
and in regions 2p16 and 2p21.
Methods: 105 lung cancers (43 AC, 35 SCC, 9 LCLC, 12 SCLC, and 
6 others) were studied for chromosome 2 count using CEP-2 (alpha 
satellite) DNA probe for ﬂuorescence in situ hybridization (FISH). 82 
of these lung tumours were studied using FISH for DNA copy number 
changes in 2p16 and/or 2p21 with one to four BAC probes. Addition-
ally, 28 of the lung tumours were microdissected and analysed by frag-
ment analysis with 15 microsatelllite markers in the 2p21-p16 region. 
Allelic imbalances were determined and compared with the copy 
number results.
Results: The average of the chromosome 2 count (CEP-2 signal) 
among the 105 lung tumours was 2.7 (range 1.7 to 5.0). CEP-2 mean 
signal count varied among the lung tumour types as follows: AC: 2.6; 
SCC: 2.8; LCLC: 3.0; SCLC: 2.4. The average DNA copy number 
among the 82 tumours studied was 2.7 also in 2p16 region, studied us-
ing three different BAC probes for FISH (n= from 28 to 69 for different 
probes). In contrast, in 2p21 the DNA sequences seemed to be gained 
with respect to the CEP-2 probe in lung tumours (n=39). The average 
copy number among all lung tumours was 4.0. The mean signal count 
at 2p21 between the lung tumour types did not differ signiﬁcantly. The 
ratio between the 2p21 locus signals and CEP2 varied from 1.4 to 1.8, 
showing a low copy number gain in 2p21 in all lung tumour types.
The frequency of allelic imbalance among informative cases was 57% 
in 2p21 and varied from 46% to 68% among different markers in 2p16. 
All lung cancer types showed some allelic imbalances in 2p.
Conclusions: Majority of lung tumours had three copies of chro-
mosome 2 in tumour cells, possibly having triploid or near triploid 
genome. In 2p21 the DNA copy number was gained with respect to the 
centromere copy number. Our results showed that the region 2p21 may 
harbour DNA sequences important to the development of lung cancer. 
The results with fragment analysis and FISH were mainly similar but 
also displayed some differences. Uniparental disomy (UPD) may cause 
some of the discrepancies. Our study also showed the importance of 
using several different techniques in quantitative molecular genomic 
studies since recognition and interpretation of low copy number gains 
are difﬁcult tasks in a complex genome such as in lung cancer.


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P2-014: Single nucleotide polymorphisms of Pim-1 in Korean lung cancer patients

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