Augustin, A.J.

Grus, F.-H.

Lutz, J.

Spitznas, M.

Zimmermann, C.W.

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P 244 Computer-supported analysis of immunomodulating activity of allopurinol in experimental lens-induced uveitis 

P243 
TITEL: POSTERIOR SUBCAPSULAR CATARACT DEVELOPMENT IN 
PATIENTS AFTER BONE MARROW TRANSPLANTATION - A CASE 
REPORT 
Junk A.K.lx2 St&&i F.H.2 Kampik A.2 Egner p.1 and Rws H * 
pumoSe: A side effect of leukemm treatment by bone marrow tmnsplantation can 
be the formation of posterior subcapsular catara~ (PSC). Total bcdy im&tion 
(TBI) More transplantation and lugh dose steroid therapy for severe graft- 
versus-host-disease (GvHD) are known to be imp&ant factors in formation of 
PSC. The purpose of this longitudinal study was the early and objective 
dadification oflens changes and their progressim over time. 
g&c&: Lwkfmia patimts wth TBI (fractionated application of 12 Gray over 
3 days) or chemotherapy pretreatment undenvmt full ophthalmologx 
investigation @atimt history, visual acuity, cioldmann applanatioll tonometry, 
biomicrosccpy of the anterior segment of the eye, especially the lens, and 
funduscopy). The Iax opacifications were assessed with a Topcon SL-45 
Scheimpflug camera with integrated rmage processing device. Contrast 
sensitivity war d&e&d under standard conditions with Pelly-Robron charts 
&I&: More than 100 patimts before and after be marrow tmnsplantation 
were examined Follow up was performed a&x six months or one year. Las 
changes in the posterior subcapsular area could be detected as early as cme year 
atIer TBI. Marked progression was found in patients after TBI when high dose 
steroid therapy had to be performed for acute OvHL3 
Conclusions: Total body irradiation sigmficantly rises the probability of posterior 
subcapsular cataract formation in b+ne marrow transplant patients. High dose 
steroid therapy also is an important factor for PSC formation. Chnical evaluation 
should mdude detection of contrast sensitivity in these patients, as it serves to 
correlate clinical tidings with patients complaints Further examination needs to 
be performed for statistical evaluation c&risk factors 
P 244 
COMPUTER-SUPPORTED ANALYSIS OF IMMUNOMODULAT- 
ING ACTIVITY OF ALLOPURINOL IN EXPERIMENTAL LENS- 
MDUCED UVEITIS 
CRUS F.-H.‘, AUGUSTLN A.J.‘, ZIMMERMANN C.W.‘, LUTZ, J. 
and SPITZNAS M.’ 
‘Dept. of Ophthalmology, University of Bonn (FRG); *Dept. of Neurology, 
University of Essen (FRG); ‘Dept. of Physiology, University of Wiirzburg 
P.3. 
wwse: The screening of sera for autoantibodies using Western blots 
&B) reveals complex staining patterns of both naturally occurring autoanti- 
bodies and disease-associated autoantibodies. Using the model of lens- 
induced uveitis (LIU) it was the aim of this study to analyse whether al- 
lopurinol changes these autoantibody (AAB) repertoires. 
Methods: We tested the sera of both LIU and control rats against WB’s 
obtained !?om SDS-PAGE preparations of protein fractions from rat lenses. 
These blots were scanned using digital image analysis (ScanPacK, Biometra, 
FRG). A newly developed technique (MegaBlot) was used to compare these 
complex AAB repertoires. 
&I& Following immunization the development of complex antibody rep- 
ertoires against lens proteins was shown. Allopurinol (AL) showed a dose 
dependent immunological effect in LIIJ treatment. A single dose of AL (50 
mgikg bw i.v.) showed no significant changes in AAB repertoire; following 
freauent aodication of AL (administered at a dose of 50 ma/kn bw i.v. everv 
two weti ‘during the imn&zation period and at a daily d&of approx. 25 
mg/kg bw orally), a significant modification of AAB repertoires against lens 
proteins was obtained as compared to those of controls. 
conclusions: Given as a single dose of 50 mgikg bw, allopurinol leads to a 
reduction of tissue inflammation by acting as a direct scavenger of free radi- 
cals and hypochlorous acid. Frequent application during the immunization 
period reveals a strong immunomodulating effect of AL Considering the 
upcoming new immunomodulating treatments for uveitis, the MegsBlot 
technique can detect and monitor theraoeuticallv induced chances in AAB 
repertdires. 
S203 


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P 244 Computer-supported analysis of immunomodulating activity of allopurinol in experimental lens-induced uveitis

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