Ismail, Ahmed

Ismail, Ashraf

Toppozada, M.

Zaki, S.

International Journal of Infectious Diseases

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S78 Abstracts, 4th DICID
and prevention. In particular, we should improve practices
of condom use and stress their importance.
PP-171 Production and comparison of high titer
lentivirus with different promoters on
regulating transgene expression in cells
L. Zhang1 *, A.Q. Wang1, H.W. Li1, C.Y. Li1. 1School of
Biotechnology, Southern Medical University, China
Objectives: Produce high titer recombinant lentivirus to
allow promising vectors to enter clinical trials and elucidate
different promoter efﬁciency on regulating transgene
expression in different cells.
Methods: Three lentiviral vector systems were constructed
by inserting cytomegavirus (CMV) promoter, human
elongation factor-1alpha (EF-1a) or ubiquitin promoter for
regulating the EGFP expression in the transduced 293A,
293FT and MOLT-4 cells. Production, concentration and
puriﬁcation of recombinant lentivirus were achieved by
developing a rapid, efﬁcient, and inexpensive method with
ultraﬁltration. Viral RNAwas quantitated by real-time PCR.
Results: Lentiviral RNA could reach a level of 7.7×108
copies/ml in the supernatant of 293FT packaging cells and
5.5×1010 copies/ml in the concentrates, respectively. CMV
promoter was more efﬁcient for regulating EGFP expression
in 293A and 293FT cells, which was about 10-fold higher than
EF-1a and ubiquitin promoters. However, EF-1a promoter
showed a higher efﬁciency than that of the CMV and
ubiquitin promoter in MOLT-4 cells. The expression of GFP
driven by CMV promoter maintained for over 4 weeks with
no apparent decrease in cell passages.
Conclusion: High titer lentiviral stocks can be produced by
an optimized ultraﬁltration method. Promoter selection and
proper host cells should be considered in order to achieve
high level transgene expression.
PP-172 Detection of the relation between the presence
of Chlamydial antigen in the cervix of infertile
women and the condition of their tubes
S. Zaki1 *, Ahmed Ismail2, M. Toppozada2, Ashraf Ismail2.
1Microbiology, Faculty of Medicine, Alexandria University,
2Obstetrics & Gynecology, Faculty of Medicine Alexandria
University, Egypt
Introduction: Chlamydia infection has now supplanted
gonorrhoea as the most common STD in the industrialized
nations. Control of this STD is particularly important
because of its grave consequences mainly infertility.
Aim: To correlate between the condition of the tube and
the presence of Chlamydial antigen.
Subjects and Methods: 100 consecutive infertile women.
Cervical specimens for C. trachomatis were collected by
rotating a cytology brush in the endocervix 360 degrees.
Chlamydiazyme and direct immunoﬂuorescent technique
were used to detect Chlamydia antigen in the cervix.
Results:
• Chlamydia trachomatis was detected in 5% of the cases.
• In 46% of cases, laparoscopy veriﬁed the presence of tubal
pathology of infertility while 54% had normal fallopian
tubes.
• 40% of chlamydia positive cases and 46.32% of chlamydia
negative cases had tubal pathology, the difference was
not statisticaly signiﬁcant, so it is apparent that not
every case with cervical Chlamydial infection leads to
tubal pathology.
• In 60% of Chlamydia positive cases the etiology of
infertility was unexplained compared to only 21.05% of
Chlamydia negative cases. This difference is statically
signiﬁcant.
Conclusion: There was no signiﬁcant relationship between
cervical Chlamydial infection and infertility.
PP-173 Isolation of Chlamydia trachomatis from cases
of cervicitis
S. Zaki1 *, A. Awaad1, N. Hanafy1, R. Malaty1. 1Microbiology,
Faculty of Medicine, Alexandria University, Egypt
Introduction: The increasing prevalence of sexually trans-
mitted diseases together with recent research ﬁndings have
led to the recognition of Chlamydiae as a frequent cause of
genital tract infections, thus adding to their known impor-
tance as agents of human diseases. The genital localization
of the agent of inclusion conjunctivitis of the newborn had
been already recognized at the beginning of this century.
Aim of the work: To establish the rate of infection with
Chlamydia trachomatis in cases of inﬂamed cervices of adult
females attending the gynecological clinics of Alexandria
Hospitals.
Subjects and Methods: 175 adult females, selected from
cases of cervicitis. Endocervical swabs were taken from
each patient through introduction of Calcium alginate swabs
(Calgi swabs), and preserved in special collection media,
then inoculated on tissue culture of McCoy cells for isolation
of Chlamydia trachomatis.
Results: Chlamydia trachomatis was isolated from four out
of the 175 cases examined 2.29%. Three of them had
cervical erosion, which may offer suitable conditions for
growth of Chlamydia trachomatis, while the fourth case
had endocervicitis.
Conclusion: It appears from this study that Chlamydia
trachomatis is present in cases of cervicitis.
PP-174 Chlamydia trachomatis in cervices of women in
late pregnancy
S. Zaki1 *, M. Said1, S. Gazaierly1, L. Kholeif2, R. Malaty1.
1Microbiology, Faculty of Medicine, Alexandria University,
2Ministry of Health Maternity Hospital, Egypt
Introduction: Chlamydia trachomatis infections of the
genital tract represent the major focus of recent interests
in Chlamydia. Assessment of the pathogenic role of
Chlamydia trachomatis in the cervix is important for
possible carcinogenic effect of deoxyribonucleic acid which
has an intracellular developmental sequence, effect on
the fetus, prenatal, intrapartum and effect on fertility if
ascending infection to fallopian tubes occurs.
Aim: Detection of Chlamydia trachomatis in cervices
of pregnant women in late pregnancy, correlation of
Chlamydial infection with clinical and cytological picture
of the cervix.
Subjects and Methods: 160 women in the third trimester
of pregnancy. Cervical scrapings were taken; one smear
was stained with Papanicolaou stain for cytology of the
cervix and the other by Giemsa stain for detection of
intracytoplasmic Chlamydial inclusions. An endocervical
swab was taken, placed in special collection medium, then
inoculated on tissue culture of McCoy cells for isolation of
Chlamydia trachomatis.
Results:
• Two Chlamydia isolates from two cases with no cervical
abnormality.
• Giemsa stained smears, showed intracytoplasmic
inclusions in four cases.
• Cervical cytology of 30 cases, showed dysplasia in one
case, metaplastic changes in 10 cases and normal cytology
in 19 cases. Two of the Chlamydia-positive cases showed
metaplastic cytological picture while the other four had
normal cytology.
Conclusion: Chlamydia trachomatis is found in the genital
tract of the pregnant females so children born to infected
mothers must be examined for Chlamydial infection of
the eye, respiratory tract and gastro-intestinal tract, since


PP-172 Detection of the relation between the presence of Chlamydial antigen in the cervix of infertile women and the condition of their tubes 

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PP-172 Detection of the relation between the presence of Chlamydial antigen in the cervix of infertile women and the condition of their tubes

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