A Normalization of Japanese cedar Pollen Specific IgE Antibody Titers Obtained from Fluorescence Enzyme Linked Immunosorbent Assay Using a Computer Program
Fluorescence enzyme linked immunosorbent assay (fluorescence-ELISA) is a more useful method than the ordinary ELISA to measure Japanese cedar pollen specific IgE antibody in sera from patients. However, the obtained data from it are often affected with some measuring conditions. In this paper, a new developed computer aided modified exponential function (y=a+be^) analysis was applied to normalize the specific IgE antibody of patients which obtained from the fluorescence-ELISA under various experimental conditions. The function well normalized the decrease of the data due to various dilution of β-D-galactosidase conjugated anti-human IgE antibodies. The mean values of the measured relative fluorescence unit (RFU) values from the sera of the 20 patients were 405+287 under the standard test condition (β-D-galactosidase cojugated anti-human IgE anbodies was used with 40 times dilution). When 50 times and 60 times dilutions of β-D-galactosidase conjugated anti-human IgE were used, the mean RFU values from patients were 335+241 and 310+280, respectively. However,the normalized mean values using computer program were 388+264, and 395+280, respectively
