Surface enhanced resonance Raman scattering (SERRS) is an analytical technique with several advantages over competitive techniques in terms of improved sensitivity and multiplexing. However, the lack of quantitative data relating to real examples has prevented more widespread adoption of the technique. Here we show multiplexed detection of two infectious diseases using a SERRS based DNA assay. SERRS active DNA probes were used in conjunction with PCR to allow simultaneous detection of Chlamydia and Gonorrhoea from urine. Silver nanoparticles were used to provide the enhancement and an assay compatible with existing instrumentation was created. Two assays have been studied and the data can be obtained in a single tube format and offers the flexibility to add further targets as desired. The technique is more sensitive than corresponding fluorescence detection and quantitative data has been obtained. This study demonstrates how SERRS can be a useful tool for meaningful analysis in molecular diagnostics
