Alizadeh, Z.

Samiei, S.

Sharifi, Z.

International Journal of Infectious Diseases

Elsevier - Publisher Connector 

S76 Poster Presentations
(30.7%), daily laborers (22.7%) and students (20%). All were
ﬁrst time donors, and 75% of the donations were replace-
ment donations. The prevalence of HIV, Hepatitis B surface
antigen (HBsAg) and Hepatitis C virus (HCV) infections were
4.5% (95CI: 3.0 6.6), 8.2% (95CI: 6.2 10.7) and 5.8% (95CI:
4.2 8.1) respectively. In univariate analysis, HCV and HIV
infections were associated (OR: 5.36, 95CI: 2 14.3).
Conclusion: The prevalence of TTIs among blood donors
is very high and the majorities of blood donors are
replacement or paid donors with one or more of the risk
factors for TTIs implying that blood transfusion is unsafe.
These ﬁndings call for the urgent implementation of the
national strategy of safe blood transfusion in Ethiopia.
Poster Presentation Diagnosis & Laboratory
Systems Development
PP-068 Survey of Anti-HBc and Anti-HBs prevalence in
HBsAg-negative blood donors in Tehran
Z. Alizadeh*, Z. Shariﬁ, S. Samiei. Iranian Blood Transfusion
Organization-Research Center, Iran
Background: Current serological screening for blood-borne
hepatitis viruses has reduced the risk of post-transfusion
hepatitis dramatically. Occult hepatitis B virus (HBV)
infection might allow the release of viremic units into the
blood supply network if blood is tested only for hepatitis B
surface antigen (HBsAg). The screening for antibody to HBc
(anti-HBc) has been shown as an alternative test for the
detection of HBV infection.
Objective: The aim of this study was to evaluate HBV
infection markers prevalence in HBsAg-negative blood
donors.
Methods: In this descriptive cross-sectional study (in 2007),
2000HBsAg-negative samples were collected from blood
transfusion centers in Tehran. All HBsAg-negative samples
were tested for anti-HBc using ELISA method. Then all
HBsAg-negative and anti-HBc-positive samples were tested
for anti-HBs by the same method. Data were analyzed
statistically using chi-square test.
Results: 199 out of the 2000 HBsAg-negative blood donors
(9.95%) were anti-HBc-positive. Out of the 199 anti-HBc-
positive samples tested for anti-HBs, 149 were anti-HBs-
positive (75%), and 100 had an antibody titer greater than
100 IU/mL (50.3%).
Conclusion: In our study, the prevalence of anti-HBc
in HBsAg-negative blood donors was high. While anti-
HBc-positive blood may be a potential source of HBV
transmission, routine application of anti-HBc screening is
not feasible in our country, as it would seriously limit the
blood supply. Therefore more sensitive techniques such as
Minipool PCR testing after virus enrichment is essential for
detecting HBVDNA in HBsAg-negative chronic HBV carriers.
PP-069 Development of RFLP-PCR assay to identify
Aspergillus species isolated from clinical and
environmental specimens
K. Diba1 *, H. Mirhendi2, N. Jalalizand3, A. Namaki4.
1Department of Parasitology and Mycology, Faculty of
Medicine, Urmia University/Medical Sciences, Urmia, Iran,
2Department of Parasitology and Mycology, shool of Public
Healths, Tehran University/Medical Sciences, Tehran,
Iran, 3Molecular Biology Center, Esfehan Health Research
Center, Tehran University/Medical Sciences, Esfehan, Iran,
4Harsin Hospital, Kermanshah University/Medical Sciences,
Kermanshah, Iran
Aspergillus species are most abundant and widely
distributed in soil, water, air, seed and food. These species
are associated with allergic bronchopulmonary disease,
mycotic keratitis, otomycosis, nasal sinusitis and invasive
infection.In this study we attempted to set up a PCR-RFLP
for identiﬁcation of the most common Aspergillus species.
Test samples were collected from clinical specimens
including sputum, sinus discharge, bronchoalveolar lavage,
nail scrapped and environmental specimens including
hospital wards and outdoors. PCR products ITS regions
in rDNA gene were digested directly and individually by
the restriction enzyme MwoI by incubation at 37ºC, then
subjected to electrophoresis in a 2% agarose gel and then
were visualized with a UV System Gel Document. Totally
205 Aspergillus isolates of our study included; 153 (75%)
environmental and 52 (25%) clinical isolates. A. ﬂavus
isolated more frequently from environmental samples
112 (55%) followed by A. niger 65 (31.7%), A. fumigatus
18 (8.7%), A. nidulans and A. parasiticus 2 (1%). RFLP
method using restriction enzyme MwoI was successful to
discriminate eight medically important Aspergillus species.
We concluded that our PCR-RFLP method using the
restriction enzyme MwoI is a rapid (during 8 10 hours)
and reliable test for colony identiﬁcation of at least the
most important Aspergillus species.
Table 1: The fragment size of ITS1-ITS2 PCR products after digestion with
the enzyme MwoI for various Aspergillus species
Species cutting size
A. fumigatus 207, 125, 108, 29, 21, 9
A. ﬂavus 325, 98, 65, 40, 20
A. niger 192, 175, 120, 108, 30, 21, 9
A. terreus 220, 109, 106, 96, 29, 9
A. nidulans 162, 135, 104, 31, 29, 9
A. clavatus 210, 125, 106
A. ochraceus 420, 90, 39, 9
A. amstelloidami 286, 106, 100, 29, 9
A. ﬁcheri 200, 140, 120
Table 2. The frequency of Aspergillus species isolated from clinical and
environmental sources
Species No Aspergillus strains identiﬁed by RFLP
Total no. of
specimens
Environmental
specimens
Clinical
specimens
No. % No. % No. %
A. ﬂavus 36 69.2 76 49.7 112 55
A. niger 4 7.6 61 39.8 65 31.7
A. fumigatus 7 13.5 11 7.2 18 8.7
A. nidulans 1 1.9 1 0.6 2 1.0
A. tereus 1 1.9 0 0 1 0.5
A. parasiticus 1 1.9 1 0.6 2 1.0
A. penicilloid 0 0 1 0.6 1 0.1
A. tamarii 0 0 1 0.6 1 0.5
A. ochraceus 1 1.9 0 0 1 0.5
A. sojae 1 1.9 0 0 1 0.5
A. niveus 0 0 1 0.6 1 0.5
Total 52 100 153 100 205 100
Acknowledgments: The author thanks Professor K. Makimura
(Institute of Medical Mycology, Teikyo University, Tokyo,
Japan) for the standard Aspergillus species and thanks
Kh. Omidi and A. Norouzinejad (Mycology lab of Institute
of Health Research of Tehran University) for their
assistance.This work was supported by the Research project
of Dr. S.H. Mirhendi proposed to Institute of Health Research
of Tehran University.


PP-068 Survey of Anti-HBc and Anti-HBs prevalence in HBsAg-negative blood donors in Tehran 

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PP-068 Survey of Anti-HBc and Anti-HBs prevalence in HBsAg-negative blood donors in Tehran

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