Heng Wu

Jiayong Zhang

Le Huang

Ling Qin

Nan Wang

Xinluan Wang

Zhijun Yang

Journal of Orthopaedic Translation

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Advanced musculoskeletal imaging systems adopted for study a novel bone-targeting delivery system carrying osteopromotive phytomolecule(s) developed for prevention of estrogen depletion induced osteoporosis

Huang, Le

Wang, Xinluan

Wang, Nan

Wu, Heng

Zhang, Jiayong

Yang, Zhijun

Qin, Ling

Elsevier - Publisher Connector 

Abstracts 235Results: Figure-1 shows a spatial normalization example and a mean BV/TV
map. Cortical thickness maps of a scan-rescan example, mean cortical thick-
ness maps, and a laminar vBMD example are shown in Figure-2. No signifi-
cantly different voxels or vertices were found after FDR correction,
indicating no significant multi-parametric differences between “baseline”
and “follow-up” scans. Global reproducibility yielded CVRMS of 1.6%
(0.018mm) and 0.9% (0.013mm) for apparent thickness, 1.5% (0.014mm)
and 0.9% (0.010mm) for SIT, and 1.2% (4.68mg/cm3) and 0.8% (3.05mg/
cm3) for vBMD, for the radius and tibia, respectively.
Conclusion: Advanced 3D image analysis techniques may be employed for
population-based local multi-parametric comparisons of HR-pQCT studies
with high reproducibility.
Acknowledgments
NIH-NIAMS: R01AR064140-R01AR060700.Figure 1. Cross-sections of voxel-wise analyses: A) Representative homoge-
nized BV/TV map. B) Reference shape. C) Map in A after spatial normaliza-
tion to B. D) Mean “baseline” BV/TV map.
Figure 2. Surface-based analysis: A) Representative “baseline” and “follow-
up” apparent cortical bone thickness maps. B) Representative “baseline”
and “follow-up” SIT maps. C) Mean apparent cortical bone thickness map.
D) Mean SIT map. E) Representative laminar vBMD maps: periosteal layer,
middle layer, and endosteal layer.IBDW2014-00138-F0061
HIGH RESOLUTION COMPUTED RADIOGRAPHY OF CORTICAL BONE
POROSITY IN WEIGHT BEARING AND NON-WEIGHT BEARING LONG BONE
SHAFT UNDER HABITUAL LOADING
Chung Ning Lam a, Yau Ming Lai a, Vivian Wing Ying Hung b, Ling Qin b
aDepartment of Health Technology and Informatics, The Hong Kong
Polytechnic University, HKSAR, PR China
bDepartment of Orthopaedics and Traumatology, The Chinese University of
Hong Kong, HKSAR, PR China
Objective: Differential mechanical loading in weight bearing and non-
weight bearing long bone could result in adaptation of bone mineral density
and microarchitecture. Understanding of the bone adaptation could shed
light into the bone remodelling processes underpinning the adaptation.
This study used high resolution computed radiography and post-image
enhancement approach to quantify the intracortical porosity (ICP) of weight
bearing and non-weight bearing long bone shafts under lifetime habitual
loading at distal tibia and radius regions, respectively.
Methods: Cortical bone segments of 7.5 mm in length at distal tibia and
radius were harvested from 20 Chinese cadavers aged 52 to 92. These
were processed and embedded in methyl methacrylate (MMA) without decal-
cification. Bone slices of 500 mm were then cut from the MMA embedded
bone specimens. Images of the bone slices were acquired using high resolu-
tion computed radiography with magnification technique. This rendered an
in-plane resolution at 100 mm. Segmentation and quantification of cortical
porosity from the mineralised tissues was achieved using Image J (National
Institute of Health).
Results: The study demonstrated that tibial ICP (27.03  2.93%) was 33%
significantly higher than that of radius (20.37  2.43%) (p < 0.001). The
ICP correlated significantly with age, with radius showing strong correlation,
r (17)Z 0.75, p < 0.001, whereas tibia showing moderate correlation, r (18)
Z 0.44, p Z 0.06. Two-way analysis of variance was carried out to study if
there were any interaction effects of bone region (tibia and radius) and bone
sector (anterior, posterior, medial, lateral cortices) on ICP. There was a
marginally significant interaction between bone region and sector on ICP
(p Z 0.09). Follow-up test showed a marginally significant simple main ef-
fects for different sectors only in tibia (p Z 0.03). The anterior cortex
(28.26  4.68 %) had 15% higher in ICP than the posterior cortex (24.55 
5.26 %) at the distal tibia (p Z 0.003). No significant pairwise differences
were found elsewhere.
Conclusion: It suggested that ICP increased with age because of unbalanced
remodelling after mid-life. The high ICP in tibia may be a result of bone
remodelling in response to dynamic loading so as to reduce the expenditure
of mechanical energy for locomotion. Sectorial differences between the
anterior and posterior cortex in distal tibia exemplified the bone adaptation
to differential tension and compression strain mode, respectively.
IBDW2014-00139-F0062
ADVANCED MUSCULOSKELETAL IMAGING SYSTEMS ADOPTED FOR STUDY
A NOVEL BONE-TARGETING DELIVERY SYSTEM CARRYING
OSTEOPROMOTIVE PHYTOMOLECULE(S) DEVELOPED FOR PREVENTION
OF ESTROGEN DEPLETION INDUCED OSTEOPOROSIS
Le Huang a, Xinluan Wang a,c, Nan Wang c, Heng Wu d, Jiayong Zhang b,
Zhijun Yang b, Ling Qin a,c
aMusculoskeletal Research Laboratory, Department of Orthopaedics and
Traumatology, The Chinese University of Hong Kong, Hong Kong SAR, PR
China
bSchool of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR,
PR China
cTranslational Medicine R&D Center, Shenzhen Institutes of Advanced
Technology Shenzhen, PR China
dDepartment of Medicine, University of Minnesota, Minneapolis, USA
Objectives: To evaluate the efficacy of Icaritin with targeting liposome de-
livery system on prevention of estrogen depletion induced osteoporosis in
vivo by analyzing the bone quality and microarchitecture by micro-
Computed Tomography (micro-CT) and the distribution of the delivery sys-
tem by using Xenogen IVIS spectrum system.
236 AbstractsMethods: (ASP8)-Liposome-Icaritin was synthesized by thin film evaporation
method with extruding through polycarbonate filter membranes to obtain
unilamellar vesicles with bone targeting molecules ASP8 attached. Eighty
four-month-old C57/BL6 female mice were divided into 8 groups (nZ10):
Baseline (BL), Sham surgery (SH), Ovariectomized (OVX), Estradiol for oral
administration(O-E2), Icaritin for oral administration (O-ICT), low dose
(8mg/kg, once a week) targeting delivery system with Icaritin injected via
caudal vein (IV-LIP+ICT+ASP8-L), high dose (8mg/kg, twice a week) targeting
delivery system with Icaritin injected via caudal vein (IV-LIP+ICT+ASP8-H),
delivery system with Icaritin injected via caudal vein (IV-LIP+ICT, 8mg/kg,
twice a week). Administrations of gavage and IV injection were applied
respectively for 6 weeks from the day right after the OVX surgery. Lumbar
spine and lower limbs were harvest 6 weeks after surgery for bone quality
analysis. The 5th vertebra body of lumbar region was scanned by micro-CT
(Scanco micro-CT 40). Trabecular bone was identified and parameters
were analyzed for evaluation of bone quality and microarchitecture. For
confirming the specificity of the targeting delivery system, Xenogen IVIS
spectrum was used to semi-qualify the distribution of bone targeting system
ex vivo by injecting labelled targeting delivery system.
Results: By comparing to the OVX group, bone quality in groups with IV injec-
tion were enhanced reflected in the increased BMD (p<0.05), bone volume
(p<0.05), trabecular bone number and connectivity density (p<0.05) and
decreased in trabecular bone separation (p<0.05) . Also the efficacy of the
targeting icaritin delivery system tended to be dosage dependent (BV
increased 14.16% in high dose group, Tb.N increased 10.34% and connectivity
density of trabecular bone increased 19.70%). Moreover from Structure Model
Index (SMI) value, we concluded that the morphology of trabecular bone in
icaritin injection groups tends more to be plat-like: SMI(OVX)Z 2.190.30,
SMI(IV-AP8+LPS+ICT-1)Z 2.070.36, SMI (IV-AP8+LPS+ICT-H)Z 2.010.23).
More signals retain in bone 72 hours after injection by comparing to the deliv-
ery system without bone targeting molecules ASP8 shown in IVIS image.
Conclusion: The novel bone-targeting delivery system carrying osteopromo-
tive phytomolecule(s) Icaritin was confirmed that was capable of preventing
the estrogen depletion induced osteoporosis in a dose dependent manner.
Acknowledgement: This study was supported by Hong Kong General
Research Fund (GRF CUHK-473013).
Micro-CT 3D images of trabecular bone in L5.IBDW2014-00140-F0063
FRACTURE RISK OF ELDERLY POPULATION: LIKELIHOOD OF
OSTEOPOROTIC FRACTURE RISK IN ELDERLY POPULATION AND INDOOR
AIR POLLUTION
Xiaodong Pei a, Zhiyi Huo b, Lijun Pei c
Department of Orthopedics, Fourth Hospital of Baotou, Inner Mongolia,
ChinabDepartment of Medical Imaging, Haidian Hospital, Beijing, China
cInstitute of Population Research, Peking University, Beijing, China
Background: Harmful particulate contaminants (such as PM2.5) that indoor
coal combustion produced can increase the incidence of cardiovascular dis-
ease and risk of death, while there is considerable evidence that cardiovas-
cular disease, dyslipidemia, and osteoporosis among elderly population have
common risk factors. Our hypothesis was that the osteoporotic fractures
may be associated with indoor air pollution. The purpose of this study was
to explore the risk of osteoporotic fracture of elderly population associated
with likelihood of indoor air pollution.
Methods: Forty-eight cases with osteoporotic fractures and 91 controls were
selected from the elderly population in Ordos region, Inner Mongolian. The
risk factors were compared between the two groups in demographic charac-
teristics, exercise, sleep, room environmental conditions, dietary, behav-
ioral habits, history of medicine use, bone disease and chronic disease by
using descriptive analysis. Multivariate logistic regression model was per-
formed to analyze environmental risk factors associated with the risk of
fractural etiology.
Results: The age range of case-control groups was 60-88 years old. There
were no statistically significant differences between two groups in age,
sex and occupation (p>0.05). The variables with statistically significant dif-
ferences in the univariate analysis were included in Logistic Regression
model. After adjustment for the education, ethnic factors, health status
during childhood, behavioral habits, history of bone disease and medicine
use, the findings showed that the exposure factors, such as indoor air condi-
tions during the winter heating, weekly exercise, sleep quality, consumption
of carbonated beverages, were associated with risk of elderly fracture.
When compared to elderly population who had good air quality of habitable
room in winter, elderly population with poor air quality had an elevated risk
for fracture (OR 40.29,95% CI 8.75-185.38); elderly population who had no
weekly exercises had an increased risk for fracture (OR 4.28, 95% CI 1.20-
15.24) when compared to those who had weekly exercises; elderly persons
with poor quality of sleep was associated with a 6.85-fold increase in risk
of fracture when compared to those with better quality of sleep (95% CI
1.61-29.14).
Conclusion: The findings suggest that poor indoor air quality during the
winter heating may be the risk factors of osteoporotic fractures for the
elderly population. It is necessary to further investigate those factors in a
larger sample population.
IBDW2014-00141-F0064
EFFECT OF ANTHROPOMETRIC ADJUSTMENTS ON BMD AND BMC Z-
SCORES IN A POPULATION OF PRADER-WILLI SYNDROME PEDIATRIC
PATIENTS
Amanda E. Marker a, David F. Short a, Talia Eldar-Geva b, Harry J. Hirsch b,
Varda Gross-Tsur b, Maayan Tiomkin c, Ari Zimran c, Thomas
N. Hangartner a
aBioMedical Imaging Laboratory, Wright State University, Dayton, OH USA
bMulti-disciplinary PWS Clinic, Shaare Zedek Medical Center, Jerusalem,
Israel
cGaucher Clinic, Shaare Zedek Medical Center, Jerusalem, Israel
Objective: Two adjustment models were applied to a dataset of bone-min-
eral density (BMD) and bone-mineral content (BMC) from dual-energy x-ray
absorptiometry (DXA) measurements of pediatric patients with Prader-Willi
Syndrome (PWS). One model used weight, height and % body fat (WHF) in
the calculation of Z-scores in addition to race, sex and age already included
in the standard model. As percent body fat is not always available, a second
model with only weight and height was also examined (WH).
Methods: Fifty-six patients with PWS, a neurogenetic disorder caused by
the absence of paternal expression of imprinted genes localized in the
15q11-q13, were recruited. PWS is characterized by an insatiable appetite
leading to obesity, short stature, cognitive and behavioral problems, hypo-
gonadism and osteoporosis. All patients had spine and hip DXA measure-
ments; 31/56 also had whole-body measurements (needed for body-fat
correction). Several patients show lower than normal height or higher
than normal weight for age. The percent body fat for all patients is either
at the upper limit of or above the normal range. Patients with extreme
anthropometric values were most affected by the anthropometrically cor-
rected Z-scores.
Results: In the analysis of Z-scores, patients below -2.0, the critical region,
are of most interest. By analyzing which patients cross the -2.0 boundary


Advanced musculoskeletal imaging systems adopted for study a novel bone-targeting delivery system carrying osteopromotive phytomolecule(s) developed for prevention of estrogen depletion induced osteoporosis 

Advanced musculoskeletal imaging systems adopted for study a novel bone-targeting delivery system carrying osteopromotive phytomolecule(s) developed for prevention of estrogen depletion induced osteoporosis

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