Chawla-Sarkar, M.

Mandal, P.

Mullick, S.

International Journal of Infectious Diseases

Elsevier - Publisher Connector 

Detection and molecular characterization of unusual rotavirus group A genotypes G12P[11] and G10P[14] in hospitalized children with acute gastroenteritis in Kolkata, India 

446 17th International Congress on Infectious Diseases / International Journal of Infectious Diseases 45S (2016) 1–477
con was transfected into Huh 7.5 cells for production of infectious
HCVparticles. The culture supernatantwas collected to infect naive
Huh 7.5 cells. siRNAs were targeted against NS5B region of HCV
genome as well as cellular factors in order to down regulate HCV
in cell model. Furthermore, multiple combinations of siRNAs were
used to observe the additive HCV down regulation.
Results: Down regulation of La autoantigen, PSMA-7, hVAP-A
and NS5B genes resulted in inhibition of HCV replication by about
65%, 30%, 35% and 40% respectively. Combination therapies of siR-
NAs against La autoantigen with NS5B and La autoantigen with
hVAP-A resulted in ∼ 85% inhibition in HCV replication.
Conclusion: Our ﬁndings indicate that in addition to HCV-
speciﬁc siRNAs, siRNAs targeted to host cellular genes have showed
promising down regulation in HCV replication. We also showed
that simultaneous silencing of more than one target is more effec-
tive than silencing a single target to inhibit the viral replication.
Therefore, multiple combinations of siRNAs against both the virus
and host genes are likely to be a potent approach in the treatment
of chronic hepatitis C.
http://dx.doi.org/10.1016/j.ijid.2016.02.945
Type: Poster Presentation
Final Abstract Number: 43.210
Session: Poster Session III
Date: Saturday, March 5, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Detection and molecular characterization of
unusual rotavirus group A genotypes G12P[11]
and G10P[14] in hospitalized children with
acute gastroenteritis in Kolkata, India
P. Mandal ∗, S. Mullick, M. Chawla-Sarkar
National Institute of Cholera and Enteric Diseases,
Kolkata, India
Background: Group A rotavirus induced acute gastroenteri-
tis affects infants and children <5 years globally. Predominantly
isolated rotavirus G types from human are G1, G2, G3 and G4
throughout the world including India. However, in recent years
there has been an increased detection of G9 and G12 genotypes.
Genotypes belonging to different animal origin such as G8, G10
and other rare genotypes have also been reported in humans with
low frequency.
Methods & Materials: During an on-going hospital based
surveillance study in 2014, stool samples were collected from
patients (0-80 years) admitted with acute gastroenteritis at Infec-
tious Disease (ID) Hospital, Kolkata. Presence of rotaviral VP6
protein was detected in stool samples using ELISA. The group A
rotavirus positive samples were used for RNA extraction followed
by Reverse Transcription and PCR. Cycle Sequencing and phyloge-
netic analysis were further used in the study.
Results: Sequence analysis of VP7 and VP4 gene segments of
group A rotavirus positive samples identiﬁed two unusual geno-
types namelyG10P[14] andG12P[11]. Theunusual nature of theirG
andP types ledus to characterize the remainingninegene segments
for deciphering their evolutionary dynamics.
Conclusion: Our study reports the identiﬁcation and char-
acterization of unusual group A rotavirus strains i.e. G12P[11]
and G10P[14] from Kolkata, India. The full genome sequencing
highlighted interspecies transmission and multiple reassortment
events in the origin of these strains. The G10P[14] strains pos-
sessed genomic constellation commonly found in artiodactyls and
therefore probably have a zoonotic origin. The G12 strains pre-
dominantly carry Wa-like genomic backbone while P[11] type is
derived from DS-1-like rotavirus strains and therefore possible
reassortment events between the two genomic backbones might
have led to the emergence of G12P[11] genotype. The study high-
lights the need for continued rotavirus surveillance and complete
genetic characterization for monitoring unusual rotavirus strains
and understanding their evolutionary origin.
http://dx.doi.org/10.1016/j.ijid.2016.02.946
Type: Poster Presentation
Final Abstract Number: 43.211
Session: Poster Session III
Date: Saturday, March 5, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Co-circulation of all four dengue virus serotypes
with concurrent infections in a single dengue
season
B. Mishra1,∗, J. Turuk1, S.J. Sahu1, A. Khajuria1, S.
Kumar2, A. Dey1, A.K. Praharaj1
1 All India Institute of Medical Science, Bhubaneswar,
India
2 KIIT University, Bhubaneswar, India
Background: Dengue is one of the notable mosquito borne
viral infections of public health concern. Dengue viruses (DENV)
belongs to the genus Flavivirus and family Flaviviridae and has
four antigenicaly related serotypes designated as DENV 1- 4. All
the four serotypes can cause clinical manifestation ranging from
mild self-limiting illness to severe dengue hemorrhagic fever (DHF)
and dengue shock syndrome (DSS). However, severity in dengue
viral infection is known to be affected by secondary infection with
heterologous antibodies or with certain dengue virus serotypes
and genotypes. This necessitates the study of circulating dengue
serotypes in a particular locality. The present study reports for the
ﬁrst time the circulation of all the four dengue serotypes alongwith
concurrent infections in an eastern state of India.
Methods & Materials: A total of 148 samples were received
from clinically suspected dengue patients during September to
December, 2014.
All the 148 samples were subjected to dengue speciﬁc MAC
ELISA (Pan Bio, Australia), and NS1 antigen detection by ELISA (Pan
Bio, Australia) for detection of dengue IgM antibody and dengue
NS1 antigen respectively. Twenty early acute samples (<3days of
illness) received were subjected for detection of dengue viral RNA
and serotyping using type speciﬁc nested multiplex RT-PCR.
Results: Twenty ﬁve sampleswere positive for dengue serology
(dengue NS1 and/or dengue IgM Ab). Five samples were found to
be positive for dengue viral RNA by RT-PCR. The type speciﬁc PCR
revealed, Dengue type 2 (DENV-2) in 2 samples, DENV-4was found
in one and 2 samples were co-infected with DENV-1 and DENV-3.
All the dengue positive patients had dengue fever and none had
dengue hemorrhagic fever.
Conclusion: The present study reports for the ﬁrst time the
co-circulation of all the four dengue serotypes along with rarely
detected DENV-4 for the ﬁrst time from eastern India.
http://dx.doi.org/10.1016/j.ijid.2016.02.947


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Detection and molecular characterization of unusual rotavirus group A genotypes G12P[11] and G10P[14] in hospitalized children with acute gastroenteritis in Kolkata, India

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