Abdelkerem, A.

Birinci, I.

Bonabi, E.

Caliskan, R.

Dinc, O.

Habip, Z.

Islak Mutcali, S.

Kırkoyun Uysal, H.

Kocazeybek, B.

Kosan, E.

Kuskucu, M.

Mete, B.

Midilli, K.

Saribas, S.

Yuksel, P.

International Journal of Infectious Diseases

Elsevier - Publisher Connector 

272 17th International Congress on Infectious Diseases / International Journal of Infectious Diseases 45S (2016) 1–477
Type: Poster Presentation
Final Abstract Number: 42.138
Session: Poster Session II
Date: Friday, March 4, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Current problems in serologically based
diagnostic algorithm of HIV 1/2: The
re-evaluation of immunodot blot assays in HIV
1/2 veriﬁcation in Turkey
P. Yuksel1,∗, R. Caliskan1, M. Kuskucu1, S. Islak
Mutcali 2, E. Kosan3, H. Kırkoyun Uysal4, Z.
Habip1, A. Abdelkerem1, B. Mete5, S. Saribas1, E.
Bonabi6, I. Birinci3, O. Dinc1, K. Midilli 1, B.
Kocazeybek1
1 Istanbul University, Cerrahpas¸a Medical Faculty,
Istanbul, Turkey
2 Dermatological Venereal Diseases Hospital,
Istanbul, Turkey
3 Capa, Red Crescent Blood Center, Istanbul, Turkey
4 I˙stanbul University I˙stanbul Medical Faculty,
I˙stanbul, Turkey
5 Istanbul University, Cerrahpasa Medical Faculty,
Istanbul, Turkey
6 Istanbul Aydın University, Istanbul, Turkey
Background: The use of conventional (serologically based) HIV
1/2 diagnostic algorithm is controversial in recent years. In this
study, we aimed to evaluate the serum samples of patients that
have been sent to veriﬁcation tests because of repeat reactive ELISA
results and showing HIV1+HIV2 positive band pattern and also to
evaluate the position ofWestern Blot/Line-Immunoassay (WB/LIA)
veriﬁcation tests on the national HIV 1/2 diagnostic algorithm.
Methods & Materials: This study was planned as a cross-
sectional/retrospective study (January 2014 – September 2015) in
serum samples of patients who were referred to the Dermatologi-
cal Venereal Diseases Hospital, Cerrahpasa Faculty ofMedicine and
Turkish Red Crescent North Marmara District Blood Centre. The
reactivity of repeat ELISA results has been veriﬁed with WB/LIA
assays in accordance with the national algorithm. The veriﬁcated
serum samples were conﬁrmed with nucleic acid (NAT) based
assays.
Results: In the study, 3224 of 10.591 samples with repeat ELISA
reactivity (30.44%) were veriﬁed by WB/LIA for HIV infection. In
32(0.99%) of the veriﬁed serum samples, along with HIV1 bands
HIV2 gp36 bands were also detected positive. Only, 17 of the veri-
ﬁed 32 serum sampleswith gp36 bandswere repeated and no gp36
band positivity was detected by Bio-Rad Genius HIV 1/2 Conﬁrma-
toryAssay in this 17 serumsamples.Moreover, theHIV2RNAsof these
samples were also detected as negative. Therefore, the HIV1+2 co-
infection possibility in these patients has been excluded. All of the
serum samples of 32 cases were HIV1 RNA positive. The remaining
15 cases were not attainable because of various reasons.
Conclusion: The detection of false gp36 band in HIV1 infections
cause problems in the diagnoses of HIV1/2 patients. These prob-
lems in WB/LIA tests may cause delays in the diagnoses of patients
and therefore negatively impacts their psychological state. In this
respect, we suggest that the WB/LIA results have to be evaluated
from this aspect and the addition of assays that can produce faster
results (peptide-based immunochromatographicmethods that dis-
tinguish HIV1/2, NAT) to Turkey’s diagnostic algorithm as present
in CDC algorithm may be suitable in these situations.
http://dx.doi.org/10.1016/j.ijid.2016.02.604
Type: Poster Presentation
Final Abstract Number: 42.139
Session: Poster Session II
Date: Friday, March 4, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Uptake of intermittent preventive therapy
among pregnant women attending antenatal
clinics in public and registered private health
facilities in Oyo State, Nigeria
A.O. Akpasa1,∗, A. Adebayo2, J. Onyilo3, B.
Ahmed4, T.O. Ladipo5, Y. Oke6, A.
Karim-Mohammed3, O. Adeusi7, B. Aiyenigba8
1 Malaria Consortium/Malaria Action Program for
States, Ibadan, Oyo, Nigeria
2 Malaria Consortium/Malaria Action Program for
States, Ibadan, Nigeria
3 Malaria Consortium/Malaria Action Program for
states, Nigeria, Ibadan, Nigeria
4 FHI360/ Malaria Action Program for States, Nigeria,
Ibadan, Nigeria
5 Oyo State Malaria Control Programme, Ibadan,
Nigeria
6 Malaria Consortium/Malaria Action Program for
states, Nigeria, Abuja, Nigeria
7 PMI Malaria Action Program for States, Abuja,
Nigeria
8 FHI360/ Malaria Action Program for States, Nigeria,
Abuja, Nigeria
Background: Malaria infection during pregnancy remain a
major public health problem in Nigeria. The 2013 Nigeria Demog-
raphy and Health Survey revealed that while most pregnant
women (PW) access Antenatal Care (ANC) from skilled care
providers, majority of them do not beneﬁt from interventions
to prevent malaria. Intermittent Preventive Therapy (IPTp) using
Sulphadoxine-Pyrimethamine (SP) is a full therapeutic course of
intermittent medicine given to PW at routine ANC visits. The WHO
recommends that this treatment be given to all PW at each sched-
uled antenatal care visit except during theﬁrst trimester. InNigeria,
the national guidelines and strategies for malaria prevention and
control during pregnancy has been revised to reﬂect WHO recom-
mendations.
Methods & Materials: This study utilized secondary data from
the routine national District Health Information System which
houses the health management information system to assess IPTp
uptake among PW who attended ANC in public and private health
facilities (HFs) inOyoStateNigeria fromOctober2014 toSeptember
2015. The national data system is able to report only two doses.
Descriptive statistics was performed to assess IPTp uptake from
reporting HFs within the period.
Results: A total of 122,320 pregnantwomen attended antenatal
clinics in 1139 public and private HFs; reporting rate was 93.3% for
all health facilities; 99% for public and 84% for private during the
period. Overall, 54% (75.1% public, 28.4% private) of ﬁrst ANC atten-
dees received IPTp1; while only 20% (75.5% public, 24.9% private)
received IPTp2. Sixty-four percent attended ANC for a minimum of


Current problems in serologically based diagnostic algorithm of HIV 1/2: The re-evaluation of immunodot blot assays in HIV 1/2 verification in Turkey 

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Current problems in serologically based diagnostic algorithm of HIV 1/2: The re-evaluation of immunodot blot assays in HIV 1/2 verification in Turkey

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