Kluger, M. J.

McCarthy, Donna O.

Shlafer, M.

Strainer, J. C.

English

epublications@Marquette

Marquette Universitye-Publications@MarquetteCollege of Nursing Faculty Research andPublications Nursing, College of1-1-1985Enhanced Hydrogen Peroxide Generation byHuman Neutrophils Following Exercise [Abstract]J. C. StrainerUniversity of Michigan - Ann ArborDonna O. McCarthyMarquette University, donnalee.mccarthy@marquette.eduM. ShlaferUniversity of Michigan - Ann ArborM. J. KlugerUniversity of Michigan - Ann ArborPublished version. Federation Proceedings, Vol. 44 (1985): 1546. Publisher Link. © 1985 Federationof the American Society for Experimental Biology. Used with permission.6641 ENHANCED HYDROGEN PEROXIDE GENERATION BY HUMAN NEUTROPHILS FOLLOWING EXERCISE. J .C. Strainer I 0.0. McCarthy I t·1. Shlafer and M. J . Kluge r t Depts . Physiology and Pharmacology . The University c f Michigan Medical Scheol, Ann Arbor 48109. Exercise may increase host defenses against infection by increasing neutrophil production of cytotoxic oxygen metabo-lites , i ncludi ng hydroge n peroxide (HP) . Five healthy human s ubjects unde rwent 1 hr of vi gorous exercise (bicycle e rgo-meter I 50% maximum cspacity) . Heparinized venous blood sampies were taken immediately befere. immediately after and 3 hr after exercise . Each subject served as a sedentary control; blood was sampled st times corresponding to exercise studies . Ex perime nts were run st the same time each day. Neutroj>hils were isol ated by density gradi ent centrifugation and suspended in phosphate buffered sa l i ne. HP generation at 37°C was stimulated by phorbol myristate acetate (25 ng/ml; 1 mf1 azide present) and measured polarographically for 20 min . HP generation was linear du ring thi s time . Exercise acutely i nc reased rates and maximum amount s of HP formation by human ne utrophils up to 3 hr after exercise . It is not knmm whe ther exercise affects neut ro phil function i n vivQ . HP Formed (nmols/1.35 x 10 6 ce11s/20 min; mean ± S. E. ) Group Pre-Exercise Post- Exercise 3 hr Post-Exercise Sedentary 61 ± 6 53 ± 4 41 ± 7* Exer cised 56 ± 7 79 ± 9*** 77 ± 7** * P""O . 05 ; ** P""O.O l ; *** P""O.OO l vs. Pre- Exercise Val ue 

Enhanced Hydrogen Peroxide Generation by Human Neutrophils Following Exercise [Abstract]

https://epublications.marquette.edu/cgi/viewcontent.cgi?article=1248&amp;context=nursing_fac

Enhanced Hydrogen Peroxide Generation by Human Neutrophils Following Exercise [Abstract]

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