Dinleyici, E.C.

Dogan, N.

Kocman, N.U.

International Journal of Infectious Diseases

Elsevier - Publisher Connector 

358 17th International Congress on Infectious Diseases / International Journal of Infectious Diseases 45S (2016) 1–477
from two different pediatric emergency services, and randomly
chosen 219 school children of 6 and 13 years of age from differ-
ent socioeconomic environments and who did not have diarrhea.
Stool samples were prepared with native-lugol and examined and
then stained with trichrome and further examined under a light
microscope.
Genomic DNA was extracted from the stool samples using
QIAamp® DNA Stool Mini Kit The extracted DNA samples were
examined in terms of the presence of Blastocystis sp. using the real-
time PCR method with the genesig® Standard Kit (Primer Design.,
UK) designed for the quantiﬁcation of the Blastocystis G elongation
factor-1 alpha gene. The PCR was performed using seven subtype-
speciﬁc sequenced tagged site (STS) primers (SB83, SB155, SB227,
SB332, SB340, SB336 and SB337) for the genotyping of Blastocys-
tis sp. The collected data was analyzed using the SPSS (Version 17,
Chicago IL, USA).
Results: 115 samples were found positive for Blastocystis
sp.. Subtyping was successfully performed on 46 samples using
sequenced-tagged site (STS) primers and the PCR method. The
most frequently detected subtype was ST3 (43.4%) followed by ST1
(26.1%), ST4 (10.9%) and ST2 (8.7%). Themixed subtypeswere iden-
tiﬁed inﬁve samples (10.9%) as; ST1+ST3 (n=3), ST1+ST2 (n=1) and
ST2+ST3 (n=1). None of the samples had ST5, ST6 or ST7. No statis-
tically signiﬁcant difference was found between the symptomatic
and asymptomatic groups in terms of the Blastocystis sp. positivity
and the distribution of subtypes (p >0.05).
Conclusion: This is the ﬁrst study conducted to investigate the
subtype distribution of Blastocystis sp. in children in Turkey and the
ﬁndings obtained from this study are in agreementwith the related
data available in Turkey.
http://dx.doi.org/10.1016/j.ijid.2016.02.770
Type: Poster Presentation
Final Abstract Number: 43.031
Session: Poster Session III
Date: Saturday, March 5, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Detection of Blastocystis sp. Infection using
different investigation techniques in children
with or without acute diarrhea
N. Dogan1,∗, N.U. Kocman2, E.C. Dinleyici2
1 Osmangazi University medical faculty, Eskisehir,
Turkey
2 Eskisehir Osmangazi University, Eskisehir, Turkey
Background: Blastocystis is a common human intestinal para-
site with high prevalence in developing countries and one of the
causative agent for acute infectious diarrhea. Microscopic methods
such as native-lugol and trichrome staining are most frequently
used in routine diagnosis. Our study to evaluate the prevalence of
Blastocystis in children in Eskisehir, Turkey with or without acute
diarrhea, using direct microscopy, trichrome stain and real-time
polymerase chain reaction (PCR) technique.
Methods & Materials: Study was carried out between January
2011 and March 2013 in Eskisehir city center in children. They
were admitted to the emergency unit with acute diarrhea and
school children without diarrhea from seven different socioeco-
nomic backgrounds have been enrolled. Stool specimens were
investigated by routine fecal examinations in the Parasitology lab-
oratory of Eskisehir Osmangazi University Education and Research
Hospital. We investigated 961 stool samples taken from children.
Wehave a limitednumber ofmolecular tests therefore 303 samples
have been evaluated with these three methods. All of the symp-
tomatic childrens stool samples (n =84) were chosen for moleculer
test and in asymptomatic children’s stool samples (n =219) we
were chosen by randomly selection.
Results: Blastocystis were seen in 38.6% of samples with direct
microscopic examination, 35.6% of samples by the trichrome stain,
38.2% by PCR method. In symptomatic group respectively; 14.2%,
19.0% and 19.0% also asymptomatic group ratio respectively; 47.9%,
42.0%and45.6%. In the symptomatic group, compared toPCR-based
evaluation of Blastocystis infection, for direct microscopic evalua-
tion the sensitivity was 12.5% and the speciﬁcity was 85.2%, while
for trichrome staining 31.2% and 83.8% respectively while the neg-
ative predictive value was 80.5% and 83.8%, respectively. In the
asymptomatic group, compared to PCR-based evaluation of Blas-
tocystis infection, for direct microscopic evaluation the sensitivity
was 74.0% and the speciﬁcity was 73.9%, while for trichrome stain-
ing 59.0% and 72.2%.
Conclusion: PCR is a useful technique for the evaluation of fecal
samples in acute diarrhea for Blastocystis sp. Direct microscopic
evaluation can be used to rule out Blastocytis infection in children
with acute infectious diarrhea but there are needed multicenter
and large-scaled molecular and clinical studies.
http://dx.doi.org/10.1016/j.ijid.2016.02.771
Type: Poster Presentation
Final Abstract Number: 43.032
Session: Poster Session III
Date: Saturday, March 5, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
In vitro activity of different 5-nitroimidazole
derivatives and essential oils against
Trichomonas vaginalis
N. Dogan1,∗, I˙. Qoran2, M. Kurkcuoglu3
1 Osmangazi University medical faculty, Eskisehir,
Turkey
2 Eskisehir Osmangazi University, Eskisehir, Turkey
3 Anadolu University, Eskisehir, Turkey
Background: Trichomoniasis is a common sexually transmit-
ted disease (STD) caused by Trichomonas vaginalis. Treatment of
trichomoniais is usually achieved by 5-nitroimidazole derivatives.
But some resistant strains to treatment failures of metronidazole
have been reported and also numerous side effects, so it is contin-
uing the search for alternative treatments. We evaluated in vitro
effective concentrations of different 5-nitroimidazole derivatives
and essential oils against T. vaginalis
Methods & Materials: T. vaginalis was grown in TYM medium
which was supplemented bovine serum and Vitamin B12. The in
vitro minimum lethal concentrations (MLC) and the time for drug
efﬁcacy were determined 48 hour cultured. The number of tropho-
zoites were adjusted to 105 parasite/ml using hematocytometer.
Metronidazoleand and two different ornidazole were prepared at
concentrations 450 mg/ml. also standart corvacrol, Origanum vul-
gare subsp.hirtum oil and tea tree oil concentrations were prepared
at concentrations 0.1ml/10 ml in steril saline solution. The activ-
ity of trophozoites was evaluated at 0-2-24-48 hours using trypan
blue and compared to growth and effective concentrations((EC50s,
EC90s and EC100) were calculated


Detection of Blastocystis sp. Infection using different investigation techniques in children with or without acute diarrhea 

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Detection of Blastocystis sp. Infection using different investigation techniques in children with or without acute diarrhea

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