Effect of inhaled corticosteroid on TNF-α production and alveolar bone loss in Wistar rats

Abstract

AbstractObjectiveTo evaluate the effect of different concentrations of inhaled budesonide on secretion of tumoral necrosis factor-alpha (TNF-α) and on ligature-induced alveolar bone loss in Wistar rats.Materials and methodsForty-two animals were randomly divided in four groups. Control group (G1) did not receive any procedure. For the other 3 groups, alveolar bone loss was induced by placement of ligatures around the upper second molar. The contralateral molar was considered intra-group control. Group 2 (G2) was nebulized with saline solution (NaCl 0.9%). Groups 3 and 4 (G3 and G4) were nebulized with 30μg and 100μg budesonide, respectively. Administration of drugs was performed daily for 14 days. Blood samples were collected from all animals for analysis of TNF-α. The maxillae from G2, G3 and G4 were removed and defleshed with 9% sodium hypochlorite. Morphometric analysis of bone loss was performed in digital standard photographs. Statistical analysis was performed with one-way ANOVA, followed by Tukey HSD or Scheffé multiple comparison's test (significance level P≤0.05).ResultsMean alveolar bone loss values for teeth with ligature were 0.72, 0.70 and 0.77mm for Groups 2, 3 and 4, respectively. No statistically significant differences were found amongst groups with or without ligature. The production of TNF-α was 60% higher in the presence of ligature (G1 vs. G2/G3/G4). No effect was observed in TNF-α secretion after inhalation of budesonide.ConclusionInhaled budesonide in different concentrations did not alter alveolar bone loss and TNF-α secretion in male Wistar rats