Gupta, A.K.

Singh, A.

International Journal of Infectious Diseases

Elsevier - Publisher Connector 

136 17th International Congress on Infectious Diseases / International Journal of Infectious Diseases 45S (2016) 1–477
Conclusion: A review of the English-language literature
revealed only six case reports of A. meyeri empyema, being the
present case the ﬁrst one reported in Portugal. Four of six patients
underwent a surgical procedure, and the duration of antibiotic
therapy ranged from 4 to 12 months. In comparison to most of
the previous reports, the present case was diagnosed early and
was effectively drained with only a chest tube. Additionally, there
was no evidence of dissemination and symptoms and radiologi-
cal ﬁndingswere rapidly improved, demonstrating that short-term
antibiotic treatment may be attempted when the adequate man-
agement is promptly instituted according to an early diagnosis and
if there is no evidence of dissemination. In conclusion, empyema
due toA.meyeri is uncommon, andanaerobic culture of pleural ﬂuid
plays a main part in the early diagnosis of actinomycosis involv-
ing pleura. Although the loculated pleural effusion was large, early
diagnosis and successful drainage may abbreviate the duration of
treatment.
http://dx.doi.org/10.1016/j.ijid.2016.02.333
Type: Poster Presentation
Final Abstract Number: 41.140
Session: Poster Session I
Date: Thursday, March 3, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Detection of (hld) gene from staphylococcus
epidermidis strains isolated from ICU of Rasul-e
Akram hospital, Tehran-Iran
A. Ebrahimzadeh Namvar1, A. Gholami2,∗
1 Department of Microbiology, Faculty of Medicine,
Babol University of Medical Sciences, Babol, IR Iran,
Babol, Iran, Islamic Republic of
2 Student of Research Committee, Babol University of
Medical Sciences, Babol, Iran, IR Iran, Babol, Iran,
Islamic Republic of
Background: Coagulase negative Staphylococci are the most
important hospital pathogens. According to the bacterial virulence
factors such as potential ability for bioﬁlm formation and also the
emergence of methicillin-resistant strains, delta toxin may lead to
the great clinical signiﬁcant concerns. Delta toxin is encoded by the
(hld) gene and a similar system called (agr), which is responsible
for regulating.
Methods&Materials: In this study, a total of 55 isolates of inva-
sive Staphylococcus epidermidis were collected from different ICU
samples of Rasul-e Akramhospital, Tehran, Iran, due to CDC criteria
for coagulase negative staphylococci guidelines. All of the isolates
were conﬁrmed by API and delta toxin synergistic hemolysis test,
ﬁnally theprevalence of (hld) genewas estimatedbyPCRmolecular
test with speciﬁc primers which were designed by primer designer
software
Results: Amongst recovered specimens, bothblood samples and
ear wound infections with (34.5%) and (3.5%) showed the high-
est and lowest percentage respectively. The synergistic hemolysis
was evaluated (58.2%) by phenotypic method, while in genotypic
method the frequency of hld gene was determined (74.5%).
Conclusion: The prevalence of delta toxin as an important vir-
ulence factor in S. epidermidis is considered as an essential aspect
for determination of invasive strains. In similar studies the men-
tioned factor has been investigated in NICU while in present study
we have compared the both NICU and ICU wards.
http://dx.doi.org/10.1016/j.ijid.2016.02.334
Type: Poster Presentation
Final Abstract Number: 41.141
Session: Poster Session I
Date: Thursday, March 3, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Single-domain antibody selected from the
phage display library neutralizes
Escherichia coli endotoxin-induced effects on
leukocytes in vitro and in Swiss albino mice
A.K. Gupta1,∗, A. Singh2
1 Lala Lajpat Rai University of Veterinary and Animal
Sciences, Hisar, Hisar, Haryana, India
2 Lala Lajpat Rai University of Veterinary and Animal
Sciences, Hisar, Hisar, India
Background: Lipopolysaccharide (LPS), also called endotoxin,
released from G–ve bacteria has a predominant role in sep-
sis through excessive production of pro-inﬂammatory mediators.
Attempts todesign rational therapiesagainst endotoxemiaandsep-
sis continue. The objective of the present study was to assess the
ability of single-domain antibody clones selected from phage dis-
play library to neutralize LPS-induced effects onmurine andbuffalo
leukocytes in vitro and in Swiss albino mice.
Methods & Materials: Three dAb.HA clones (Cl-18, Cl-23 and
Cl-26) originally selected as LPS-binders from the phage display
library of LPS-immunized Indian desert camel were sub-cloned
in pET303/CT vector/BL21(DE3) host system and expressed as
dAb.6xHis clones. The clones were puriﬁed by Nickel-chelate chro-
matography, andconﬁrmedbySDS-PAGEand immunoblotting. The
nucleotide sequences of the clones were determined.
Results: All the dAb clones reacted with both LPS and lipid A
in indirect ELISA and exhibited thermo-stability. The afﬁnity con-
stants (Ka) of dAbCl-26, dAbCl-18 and dAbCl-23 for LPS were 4.28
x108/M, 2.18 x108/M and 2.19x108/M, respectively. Both dAbCl-
26 and dAbCl-18 decreased the LPS-induced expression of TNF,
IL-1 andMHC II genes in buffalo leukocytes, and IL-1, IL-6, CD80,
MHC-II and TNF (only Cl-26) genes in murine macrophages, but
dAbCl-23 increasedbuffalo TNF andMHC-II, and themurinegenes
as measured by RT-qPCR. The dAbCl-26 and dAbCl-18 decreased,
17th International Congress on Infectious Diseases / International Journal of Infectious Diseases 45S (2016) 1–477 137
but dAbCl-23 increased LPS-induced TNF levels in Swiss albino
mouse model.
Conclusion: In conclusion, dAbCl-26was able to neutralize LPS-
induced effects in murine and buffalo macrophages, and in vivo in
mice.
http://dx.doi.org/10.1016/j.ijid.2016.02.335
Type: Poster Presentation
Final Abstract Number: 41.142
Session: Poster Session I
Date: Thursday, March 3, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Study on the frequency of spa gene in
Staphylococcus aureus isolates from human
infections and its relationship with mecA gene
M. Haghkhah ∗, Z. Lotﬁ
Shiraz University, School of Veterinary Medicine,
Shiraz, Iran, Islamic Republic of
Background: Staphylococcus aureus is themost important cause
of nosocomial infections acquired in the community. Today, S.
aureus has been known as one of the most important global prob-
lems because of its high virulence and increasing resistance to
antimicrobial drugs. Due to the high mortality rate of nosocomial
infections associated with methicillin-resistant S. aureus, identi-
ﬁcation and knowledge of the regional model is necessary for
the proper treatment of infections caused by this organism. On
the other hand, genotyping of the isolates of this bacterium can
be widely used because we can identify the source of infection.
The molecular techniques have been developed to determine the
genetic of the isolates. One proposed method is examination of
polymorphism X region of the protein A gene (Spa) by PCR method.
This region is one of the distinguishing factors and different pat-
terns of this gene have been identiﬁed in various studies. Typing
the isolates of S. aureus using Spa gene can be a useful method for
epidemiological studies.
Methods & Materials: In this study, 115 samples of S. aureus
isolated from human infections after culture on blood agar and
mannitol salt agar and catalase and oxidase tests were examined
by PCR.
Results: PCRmethodusing Sauprimers showed that 103 (89.6%)
out of 115 isolates revealed as S. aureus. Isolates formecA genewere
96 (93.2%) positive and 7 (6.8%) negative. Ninety one (88.3%) and 12
(11.7%)were positive andnegative for Spa gene respectively. A total
of 86 (85.3%)were positive for both genes. Ten (8.9%) sampleswere
positive for mecA gene, not for Spa gene, 5 (4.8%) were negative for
mecA gene, and 2 (1.9%) were negative for both genes.
Conclusion: All differences between the groups using non-
parametric chi-square test were signiﬁcant (P=0.04). In general
conclusion, this study showed that most MRSA have virulence
genes such as Spa and play a critical role in nosocomial infections.
http://dx.doi.org/10.1016/j.ijid.2016.02.336
Type: Poster Presentation
Final Abstract Number: 41.143
Session: Poster Session I
Date: Thursday, March 3, 2016
Time: 12:45-14:15
Room: Hall 3 (Posters & Exhibition)
Meningococcal pneumonia in Japan: A case
report and review of the literatures
J. Hirai1,∗, T. Kinjo1, T. Tome2, K. Uechi2, M.
Nakamatsu1, S. Haranaga1, J. Fujita1
1 Faculty of Medicine, University of the Ryukyus,
Okinawa, Japan
2 University Hospital of the Ryukyus, Okinawa, Japan
Background: Although previous report showed that Neisse-
ria meningitidis (N. meningitidis) was detected from oral cavity of
healthy Japanese volunteers, pneumonia causedby this pathogen is
very rare in Japan.Here,wepresenta caseofnon-invasivemeningo-
coccal pneumonia and review case reports in Japan.
Methods & Materials: We searched Japanese case reports of
meningococcal pneumonia with Pubmed and the search engine
operated by JapanMedical Abstracts Society. The data including the
present case were pooled into the following categories for analy-
sis: age, sex, co-morbidities, travel history, symptom, present of
bacteremia, patterns of chest images, treatment, prognosis, and
serotype of N. meningitidis.
Results: We found 15 cases in the 9 literatures published
between 1984 and 2015. The median age of the patients was
42.0 years (range: 18 to 78 years), and men were predominant
(68.8%, 11 of 16 patients). The most common underlying con-
dition was respiratory diseases (50.0%) such as asthma, chronic
obstructive pulmonary disease, interstitial pneumonia, and diffuse
panbronchiolitis. The second most underlying disease was men-
tal disorder (25.0%). Two patients (12.5%) had a travel history.
Fever, cough, dyspnea, disturbanceof consciousness, and chest pain
were noted in 68.8%, 62.5%, 25.0%, 12.5%, 6.3%, respectively. Blood
cultureswere positive in 2 of 16 cases (12.5%), but no patient devel-
opedmeningococcemia despite the present of bacteremia. Bilateral
chest inﬁltration was observed in 2 cases (18.2%), and right- and
left-sided pneumonia were 7 (63.6%), 1 (9.1%), respectively. Beta-
lactams were used in 11 cases (78.6%). All cases were cured with
appropriate antibiotics. Serogroup B meningococci were identiﬁed
in 5 cases (31.3%). There were 3 cases of nosocomial transmission
and one case of intra-familial infection.
Conclusion: In our study, there was no meningococcal pneu-
monia with meningitis. All cases were recovered by prompt and
appropriate treatments.Wereconﬁrmedthatmeningococcalpneu-
moniawas very rare in Japan, particularly after 2009. Since national
survey of invasive meningococcal disease has started from April
2013 in Japan, the case report of meningococcal pneumonia might
be increased in the future.
http://dx.doi.org/10.1016/j.ijid.2016.02.337


Single-domain antibody selected from the phage display library neutralizes Escherichia coli endotoxin-induced effects on leukocytes in vitro and in Swiss albino mice 

file:///data/core-remote/dit/data/elsevier/pdf/28d/aHR0cDovL2FwaS5lbHNldmllci5jb20vY29udGVudC9hcnRpY2xlL3BpaS9zMTIwMTk3MTIxNjMwMzE0OQ==.pdf

Single-domain antibody selected from the phage display library neutralizes Escherichia coli endotoxin-induced effects on leukocytes in vitro and in Swiss albino mice

Abstract

Similar works

Full text

Available Versions

Elsevier - Publisher Connector