ABSTRAKIsolasi senyawa terpenoid dari ekstrak etil asetat kulit akar biduri (Calotropis gigantea) telah dilakukan. Kulit akar C. gigantea dimaserasi menggunakan pelarut metanol, kemudian dipartisi menggunakan pelarut n-heksana, dan etil asetat, sehingga diperoleh ekstrak pekat sebanyak 66,18, 20,45, dan 73,60 gram. Ekstrak etil asetat diisolasi menggunakan metode Kromatografi Kolom Cair Vakum (KKCV) sehingga diperoleh 5 fraksi gabungan, yaitu Fraksi A (0,25 g), B (0,62 g), C (0,30 g), D (2,75 g) dan E (5,80 g). Fraksi C direkromatografi menggunakan kromatografi kolom gravitasi menghasilkan 40 fraksi. Hasil uji fitokimia subfraksi C2 menggandung senyawa golongan triterpenoid dan karakterisasi menggunakan spektrometri FT-IR menunjukkan adanya gugus C-H, C=O, C=C, dan C-O (ester). Berdasarkan data spektrometri massa, diduga subfraksi C2 mengandung senyawa tarakseril asetat.Kata Kunci : Calotropis gigantea, tarakseril asetat, triterpenoid.ABSTRACTIsolation of terpenoid compound from ethyl acetate of Calotropis gigantea root bark. The root bark of C. gigantea was macerated using methanol solvent, then partitioned with n-hexane, and ethyl acetate, so obtained concentrated extracts of 66.18, 20.45, and 73.60 g. The ethyl acetate extract was isolated by Vacuum Column Chromatography method (VCC) so that obtained 5 fractions of combined, Fraction A (0.25 g), B (0.62 g), C (0.30 g), D (2.75 g) and E (5.80 g). Fraction C was recromatographed by gravity column chromatography obtained 40 fractions. The result of phytochemical test of subfraction C2 contains triterpene compound then characterized by FT-IR spectrometry showed the presence of C-H, C=O, C=C, and C-O (ester) groups. According to the characterization with mass spectrometry data, C2 subfraction expected have contained taraxeryl acetate compound.Keyword : Calotropis gigantea, taraxeryl acetate, triterpene.
