FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM UNIVERSITAS SYIAH KUALA
Abstract
Agarwood is the result of secondary metabolites of plants infected by pathogenic fungal, which is the plant genus Aquilaria. Agarwood known as wood resin has a distinctive scent and can be used as materials for medicines, cosmetics and wood incense. The aim of this study was to identify the fungal associated in the agarwood that has been in a dry state. Firstly, agarwood sterilized with a hypochloride solution then cultured in the media Potatoes Dextrose Agar (PDA) and Potatoes Dextrose Broth (PDB). Fungal is extracted using lysis buffer solution according by Sambrook et al. (1985), the quality and quantity of DNA concentration seen through agarose gel electrophoresis. Fungal isolates were amplified using primers ITS1 and ITS4, amplification product was sequenced and constructed using application MEGA 7 the algorithm is maximum parsimony and maximum likehood with repetition bootstrap 1000 times. The results showed that the sequencing results of the samples R1 is 720 bp and A1 is 659 bp. In the genotypic, R1 is fungal Rhizopus oryzae with bootstrap values of 100 and A1 is Phellinus noxious with bootstrap value 34. The two samples of the fungal is not that cause of agarwood.Keyword : Agarwood, Aquilaria, ITS, Phylogenetic tree
