Zea Ochtavia

ETD - Unsyiah Central Library

ELECTRONIC THESIS AND DISSERTATION UNSYIAHTITLEKADAR HEMOGLOBIN DAN JUMLAH ERITROSIT TIKUS PUTIH (RATTUS NORVEGICUS) STRAIN WISTARSETELAH PEMBERIAN FORMALINABSTRACTKADAR HEMOGLOBIN DAN JUMLAH ERITROSIT TIKUS PUTIH (Rattus norvegicus) STRAIN WISTAR SETELAHPEMBERIAN FORMALINABSTRAKPenelitian ini bertujuan mengetahui pengaruh pemberian formalin melalui intraperitoneal terhadap kadar hemoglobin dan jumlaheritrosit pada tikus putih (Rattus norvegicus) strain Wistar. Hewan yang digunakan dalam penelitian ini adalah 24 ekor tikus putihjantan. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) pola searah dengan 4 kelompok perlakuan.Masing-masing kelompok perlakuan terdiri atas enam ekor tikus. Kelompok kontrol tikus hanya diberi pakan dan air minum tanpaperlakuan apapun, kelompok 1 tikus diinjeksi formalin dengan dosis 1 mg/Kg bb, kelompok 2 diinjeksi formalin dengan dosis 2,5mg/Kg bb, kelompok 3 diinjeksi formalin dengan dosis 5 mg/Kg bb. Masing-masing kelompok diberi perlakuan selama 14 hariberturut-turut secara intraperitoneal. Selama penelitian tikus diberi pakan dan air minum secara adlibitum. Pada hari ke 15, sampeldarah diambil melalui sinus orbitalis menggunakan pipet kapiler. Hasil analisis statistik menunjukkan rata-rata (Â±SD) kadarhemoglobin (g/dl) K0 12,00 Â±0,72, K1 9,63 Â±1,67, K2 9,83 Â±1,30, dan K3 9,76 Â±1,41. Rata-rata (Â±SD) jumlah eritrosit(106/mm3) K0 7,06 Â±0,78, K1 5,31 Â±1,46, K2  5,35 Â±0,97, dan K3 4,98 Â±0,59. Hasil uji ANOVA menunjukkan kelompokK0 berpengaruh nyata (P0,05) antara kelompok perlakuan. Disimpulkan bahwa pemberian formalin secara intraperitoneal dapatmenurunkan kadar hemoglobin dan jumlah eritrosit tikus putih strain Wistar.Haemoglobin Levels and Number of Erythrocyte in Rats (Rattus norvegicus) Wistar strain after Formalin AdministrationABSTRACTThe aim of this study was to determine the effect of formalin administration via intraperitoneal to haemoglobin levels and todetermine the number of erythrocyte in rats (Rattus norvegicus) Wistar strain. A number of 24 male rat used in this study. Theresearch was design ed by uring completely randomized design (CRD) with 4 unidirectional treatment groups. Each group consistedof six rats. Group control rat was given no treatment in the control group. Formalin was administened by dosage of 1 mg/Kg ofbody weight in the first group, 2,5 mg/Kg of bw in second group, and 5 mg/Kg of bw in the third group. Each group was given thespecific treatment for 14 days intraperitoneally. During the study, rat were fed as adlibitum. On day 15, blood was taken throughorbital sinus by using a pipette capillary. The statistical analysis showed that mean (Â±SD) levels of haemoglobin (g/dl) were K012,00 Â±0,72, K1 9,63 Â±1,67, K2 9,83 Â±1,30, and K3 9,76 Â±1,4. Mean (Â±SD) number of erythrocyte (106/mm3) were K07,06 Â±0,78, K1 5,31 Â±1,46, K2  5,35 Â±0,97, and K3 4,98 Â±0,59. The results of ANOVA showed highly significant K0(P0,05). It was concluded that the administration of formalin intraperitoneal can decrease haemoglobin levels erythrocyte in Wistarstrain rat.

KADAR HEMOGLOBIN DAN JUMLAH ERITROSIT TIKUS PUTIH (RATTUS NORVEGICUS) STRAIN WISTAR SETELAH PEMBERIAN FORMALIN

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