Agroinfiltration of avirulence gene constructs. The response of flax cultivars and near-isogenic lines to expression of avirulence gene candidates (AvrM14-A, AvrM14-B and AvrL2-A) using Agrobacterium tumefaciens-mediated transient transformation. (PDF 2637 kb

Adnane Nemri

Adrienne R. Hardham

Ann-Maree Catanzariti

Cameron A. Jack

Claire Anderson

David A. Jones

Gregory J. Lawrence

Jeffrey G. Ellis

Muhammad Adil Khan

Narayana M. Upadhyaya

Peter N. Dodds

PubMed

AvrL2 family DNA sequences. Alignment of AvrL2 coding regions with their closest matching scaffolds in the M. lini genome assembly. Polymorphic nucleotides are shaded. (PDF 337 kb

Claire Anderson (734168)

Muhammad Khan (3412757)

Ann-Maree Catanzariti (3509984)

Cameron Jack (3509978)

Adnane Nemri (147172)

Gregory Lawrence (3509987)

Narayana Upadhyaya (783631)

Adrienne Hardham (3509981)

Jeffrey Ellis (3509993)

Peter Dodds (3509990)

David Jones (43312)

The Francis Crick Institute

Additional file 15: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

Gene segregation ratios in the CH5 F2 mapping family. Segregation ratios of avirulence genes and the I-1 inhibitor gene in the CH5 F2 family as obtained by Lawrence et al. [48]. (DOCX 17 kb

Additional file 5: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

Chromosome duplication in CH5F2-133. Graphs showing: (A) read depth and percentage of H allele reads per marker across the CH5 genetic map; and (B) mean read depth and mean percentage of H allele reads per marker in a region of chromosome duplication in the CH5 F2 individual, CH5F2-133. (PDF 137 kb

Additional file 11: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

Markers used in the CH5 genetic map. Table showing detailed information for all RADseq-derived markers in the CH5 genetic map, including marker sequences. (XLSX 1656 kb

Additional file 3: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

Linkage groups in the CH5 genetic map. Graphic representations of the 27 linkage groups in the CH5 genetic map, including the positions of the I-1 avirulence inhibitor gene and all mapped avirulence genes. (PDF 6048 kb

Additional file 4: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

Within-linkage group chimeric scaffolds. Comparison of physical and genetic maps for scaffolds that are: (A) within-linkage group chimeras; or (B) within- and between-linkage group chimeras. The positions of genetic markers are shown as vertical bars on the physical maps and these are joined to their corresponding positions in the genetic maps by a dotted line. For each gap in the genetic map, the number of markers shown represents those present in recombination bins that lie between the bins containing the markers that flank the chimeric breakpoint. (PDF 838 kb

Additional file 7: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

Springer - Publisher Connector

Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini
        

Anchored scaffolds in the M. lini genome assembly. Table showing the numbers of markers, assignment to genetic linkage group and orientation information for each anchored scaffold in the M. lini genome assembly. (XLSX 255 kb

Additional file 6: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

RADseq and genetic marker summary. Table showing the numbers of read pairs retained for analysis per CH5 F2 individual after pre-processing and removal of PCR duplicates for the NsiI and PstI RADseq sequencing libraries. The numbers of markers of each type used in the CH5 genetic map are also shown. (DOCX 19 kb

Additional file 1: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

AvrM14 sequences. DNA sequences of AvrM14-A and AvrM14-B and their alignment to sc27. (PDF 377 kb

Additional file 12: of Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

https://figshare.com/articles/Additional_file_12_of_Genome_analysis_and_avirulence_gene_cloning_using_a_high-density_RADseq_linkage_map_of_the_flax_rust_fungus_Melampsora_lini/4404851

Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini

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The Francis Crick Institute

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The Francis Crick Institute

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The Francis Crick Institute

The Francis Crick Institute

Springer - Publisher Connector

The Francis Crick Institute

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