Additional file 3. Details of primers used, experimental and theoretical molecular weights, pI values and details of post-translational modifications in GAPDH

Ajay Kumar

Ankit Sahu

Anushri Agnihotri

Chaaya Iyengar Raje

Himanshu Malhotra

Kalpesh Kumar Rajwadi

Manoj Kumar

Manoj Raje

Navdeep Sheokand

Naveen Bhagath

Navi Hasan

Pravinkumar Patil

Rachita Patkar

Vishant Mahendra Boradia

Microbial Cell Factories

PubMed

Crossref

Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

Additional file 1: Figure 2 Intact mass analysis and isoelectric point of recombinant M.tb GAPDH. MALDI-TOF confirms the presence of a single protein corresponding to a. MT-GAPDH-H and b. EC-H-GAPDH. Two dimensional gel analysis of rGAPDH purified from different source c. MT-rGAPDH d. MS-rGAPDH e. EC-rGAPDH where the gel was either stained with coomassie or western blot using rabbit α-GAPDH or mouse α-His. f. Peptide mass fingerprinting (PMF) of ~ 50 kD M. smegmatis protein that co-elutes with rPykA. Identified sequences are indicated in bold face

Vishant Boradia (3587960)

Pravinkumar Patil (3587954)

Anushri Agnihotri (3587963)

Ajay Kumar (192967)

Kalpesh Rajwadi (3587978)

Ankit Sahu (3587951)

Naveen Bhagath (3587957)

Navdeep Sheokand (3587948)

Manoj Kumar (205700)

Himanshu Malhotra (3587975)

Rachita Patkar (3587972)

Navi Hasan (3587966)

Manoj Raje (586808)

Chaaya Raje (3587969)

The Francis Crick Institute

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                     Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

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                     Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

Additional file 1: Figure 1 Expression of rGAPDH in E. coli. a. Expression of EC-H-GAPDH in cellular fractions of E. coli-GroEL/ES strain assessed by 10 % SDS PAGE and b. confirmation by western blotting using Mouse α His antibody. c. Affinity purified EC-H-GAPDH and western blot with α-GAPDH and α-His to confirm purification from E. coli host cytosol. d. ClustalW analysis of GAPDH from M.tb H37Rv, M.tb H37Ra, M. smegmatis and E. coli

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                     Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

Boradia, Vishant Mahendra

Patil, Pravinkumar

Agnihotri, Anushri

Kumar, Ajay

Rajwadi, Kalpesh Kumar

Sahu, Ankit

Bhagath, Naveen

Sheokand, Navdeep

Kumar, Manoj

Malhotra, Himanshu

Patkar, Rachita

Hasan, Navi

Raje, Manoj

Raje, Chaaya Iyengar

University of Miami: Scholarship@Miami

Additional file 4. Methodology for Peptide Mass Fingerprinting, Sequence analysis, 2D gel electrophoresis and LC MS/MS of GAPDH

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                     Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

Springer - Publisher Connector

https://figshare.com/articles/MOESM3_of___Mycobacterium_tuberculosis_H37Ra_a_surrogate_for_the_expression_of_conserved_multimeric_proteins_of_M_tb_H37Rv/4475549

Mycobacterium tuberculosis H37Ra: a surrogate for the expression of conserved, multimeric proteins of M.tb H37Rv

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The Francis Crick Institute

The Francis Crick Institute

The Francis Crick Institute

University of Miami: Scholarship@Miami

The Francis Crick Institute

Springer - Publisher Connector