Estudio molecular para la detección de Leptospira spp. en mamíferos silvestres y domésticos en la reserva ecológica Costanera Sur, CABA

Abstract

Under "One Health" concept, the health problem is approached from a holistic view linking human, animal and environmental health. Multiple pathogens circulating among animal and human populations, which can be a threatto both human and animal health. Leptospira spp. has been reported to cause infection in almost all species of mammals. In this work the human-animal-environment interface was analyzed by studying Leptospira spp. infection model in wild and domestic species in the Costanera Sur Ecological Reserve. Data were obtained through a transects, stratified stages and proportionate sampling. Urine and renal tissue were taken as appropriate and PCR diagnostic was performed. The present work has the objective to study the occurrence of leptospirosis by molecular techniques(PCR), in populations of domestic and wild animals of the RECS. At the same time, contribute to the knowledge of the impact of the contact of these populations and their potential role as reservoirs and transmitters of Leptospira spp. Which inhabit protected natural areas, modified by increasing urbanization processes, such as the RECS.A total of 140 animals belonging to 10 different species were caught:6 species of rodents, 3 species of marsupials, 1 cingulate and a total of 30canines. All analyzed samples were negative. The eco-environmental conditions of the study area posed an enabling environment for development of Leptospira spp. But we could presume that the dispersion of the populations of host (and therefore their potential reservoirs)condition the environmental burden of bacteria in different habitats, may establish a detection limit below the level of sampling and diagnostic techniques used in this research. This study is considered an approach of the knowledge of the eco epidemiology and infection risk of diseases in the human- animal - environment interface in CABA, considering Leptospira spp. as zoonotic infectious disease model, in which proposals to deepen these studies are performed.Fil: Berra, Yanina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Veterinaria en Salud Pública; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arocena, Gastón Maximiliano. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Artuso, María Carolina. Ministerio de Agricultura, Ganadería, Pesca y Alimento. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ercolini, Carina. Gobierno de la Ciudad Autónoma de Buenos Aires. Ministerio de Salud. Instituto de Zoonosis Luis Pasteur; ArgentinaFil: Bruno, Antonela. Gobierno de la Ciudad Autónoma de Buenos Aires. Ministerio de Salud. Instituto de Zoonosis Luis Pasteur; ArgentinaFil: Orozco, Maria Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Degregorio, Osvaldo Jorge. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Veterinaria en Salud Pública; Argentin