Proteolytic potential of enzymes produced by candida parapsilosis and rhodotorula. Mucilaginosa isolated from liquid whey

Abstract

The search for yeast with proteolytic activity that can be explored in technology and product innovation was the objective of this study to isolate and identify strains present in liquid whey and evaluate the proteolytic activity of isolates. From the isolated strains were selected for molecular identification, those with proteolytic activity. Verification of proteolytic activity was performed on milk agar and visualized by a translucent halo. Four strains belonging to two yeast species were identified as protease producers. Whey is promising in microbial sources of biotechnological interest and C. parapsilosis (ES01) and R. mucilaginosa (ES04) strains were good protease producers in commercial agar-like milk agar. These results indicate the proteolytic potentiality of strains isolated from whey.Fil: Monte, Aline Marques. Universidade Federal Do Piaui.; BrasilFil: Matos da Silva, Ana Karoline. Universidade Federal Do Piaui.; BrasilFil: Dourado Rodrigues, Aline Maria. Universidade Federal Do Piaui.; BrasilFil: Muratori Costa, Luciana. Universidade Federal Do Piaui.; BrasilFil: Sousa Santos, André Luis. Universidade Federal do Rio de Janeiro; BrasilFil: Gomes Abreu Bacelar, Rafael. Universidade Federal Do Piaui.; BrasilFil: de Sousa Ramos, Lívia. Universidade Federal do Rio de Janeiro; BrasilFil: Folmer Correa, Ana Paula. Universidade Federal de Roraima; BrasilFil: Brandelli, Adriano. Universidade Federal do Rio Grande do Sul; BrasilFil: Pereyra, Carina Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Sanches Muratori, Maria C.. Universidade Federal Do Piaui.; Brasi