Descriptive statistics of the phenotypic scores within the base mapping population 11-373. Powdery mildew symptoms in the field were evaluated in two subsequent years. Greenhouse, in vitro experiments and the qPCR-based molecular assay were carried out with three to four biological replicates of each seedling plant in 2014. (DOCX 14ย�kb

A Calonnec

A Doligez

A Feechan

A Untergasser

Alan C. Tenscher

Angelica Jermakow

B Bartholomew

BA McDonald

BE Stummer

C Coleman

C Délye

C Li

CL Barker

D Merdinoglu

Dario Cantu

DD Kosambi

DM Gadoury

Dosmann MS, Del Tredici P: The Sino-American Botanical Expedition of

DW Ramming

Dániel Pap

E Marguerit

E Peressotti

E Ritter

F Licausi

FJ Reifschneider

G Cipriani

G Gaspero Di

I Fechter

Ian B. Dry

J Chaib

J Montarry

J Ooijen Van

J Pauquet

JE Bowers

K Suwabe

KB Fuller

KC Amrine

KD Scott

L Jones

LJ Welter

M Brewer

M Dalbó

M Kearse

M Thomas

M. Andrew Walker

MK Monaco

O Jaillon

O Kohany

P Barba

QHZF Shen

R Eibach

R Voorrips

Róbert Oláh

S Blanc

S Hoffmann

S Mahanil

S Riaz

S Rozen

Summaira Riaz

Team RDC

TM Donald

V Decroocq

W Qiu

WS Martins

X Wang

Y Wan

Z Huang

BMC Plant Biology

PubMed

Dรกniel Pap (3533540)

Summaira Riaz (3533522)

Ian Dry (3533519)

Angelica Jermakow (3533534)

Alan Tenscher (3533525)

Dario Cantu (207551)

Rรณbert Olรกh (3533537)

M. Walker (1291794)

The Francis Crick Institute

Additional file 2: Table S2. of Identification of two novel powdery mildew resistance loci, Ren6 and Ren7, from the wild Chinese grape species Vitis piasezkii

BackgroundGrapevine powdery mildew Erysiphe necator is a major fungal disease in all grape growing countries worldwide. Breeding for resistance to this disease is crucial to avoid extensive fungicide applications that are costly, labor intensive and may have detrimental effects on the environment. In the past decade, Chinese Vitis species have attracted attention from grape breeders because of their strong resistance to powdery mildew and their lack of negative fruit quality attributes that are often present in resistant North American species. In this study, we investigated powdery mildew resistance in multiple accessions of the Chinese species Vitis piasezkii that were collected during the 1980 Sino-American botanical expedition to the western Hubei province of China.ResultsA framework genetic map was developed using simple sequence repeat markers in 277 seedlings of an F1 mapping population arising from a cross of the powdery mildew susceptible Vitis vinifera selection F2-35 and a resistant accession of V. piasezkii DVIT2027. Quantitative trait locus analyses identified two major powdery mildew resistance loci on chromosome 9 (Ren6) and chromosome 19 (Ren7) explaining 74.8&nbsp;% of the cumulative phenotypic variation. The quantitative trait locus analysis for each locus, in the absence of the other, explained 95.4&nbsp;% phenotypic variation for Ren6, while Ren7 accounted for 71.9&nbsp;% of the phenotypic variation. Screening of an additional 259 seedlings of the F1 population and 910 seedlings from four pseudo-backcross populations with SSR markers defined regions of 22&nbsp;kb and 330&nbsp;kb for Ren6 and Ren7 in the V. vinifera PN40024 (12X) genome sequence, respectively. Both R loci operate post-penetration through the induction of programmed cell death, but vary significantly in the speed of response and degree of resistance; Ren6 confers complete resistance whereas Ren7 confers partial resistance to the disease with reduced colony size. A comparison of the kinetics of induction of powdery mildew resistance mediated by Ren6, Ren7 and the Run1 locus from Muscadinia rotundifolia, indicated that the speed and strength of resistance conferred by Ren6 is greater than that of Run1 which, in turn, is superior to that conferred by Ren7.ConclusionsThis is the first report of mapping powdery mildew resistance in the Chinese species V. piasezkii. Two distinct powdery mildew R loci designated Ren6 and Ren7 were found in multiple accessions of this Chinese grape species. Their location on different chromosomes to previously reported powdery mildew resistance R loci offers the potential for grape breeders to combine these R genes with existing powdery mildew R loci to produce grape germplasm with more durable resistance against this rapidly evolving fungal pathogen

Pap, Dániel

Riaz, Summaira

Dry, Ian B

Jermakow, Angelica

Tenscher, Alan C

Cantu, Dario

Oláh, Róbert

Walker, M Andrew

eScholarship - University of California

English

Identification of two novel powdery mildew resistance loci, Ren6 and Ren7, from the wild Chinese grape species Vitis piasezkii

Crossref

A framework genetic map of Vitis piasezkii DVIT2027. Markers that have significant segregation deviation from Mendelian ratios are marked with asterisks indicating the significance levels at alpha 0.01 = *, 0.05 = **, 0.001 = ***, 0.005 = ****, 0.0001 = *****, 0.0005 = ******, and 0.00001 = *******. (PNG 186 kb

Dániel Pap (3533528)

Róbert Oláh (3533531)

Additional file 5: Figure S1. of Identification of two novel powdery mildew resistance loci, Ren6 and Ren7, from the wild Chinese grape species Vitis piasezkii

Gene and transposon annotation of the corresponding genomic region of Ren6 (A) and Ren7 (B) loci in the PN40024 sequence. The green color indicates the annotated genes labeled with gene ID and the maroon color is used for the mRNA of the corresponding gene. The blue color was used to mark the location of flanking markers on the sequence. All other colors indicate different types of transposable elements identified with the Repbase database. Transposable elements smaller than 100 bp were not included in the figure. (PNG 3624 kb

Additional file 7: Figure S2. of Identification of two novel powdery mildew resistance loci, Ren6 and Ren7, from the wild Chinese grape species Vitis piasezkii

Springer - Publisher Connector

https://figshare.com/articles/Additional_file_5_Figure_S1_of_Identification_of_two_novel_powdery_mildew_resistance_loci_Ren6_and_Ren7_from_the_wild_Chinese_grape_species_Vitis_piasezkii/4422836

Identification of two novel powdery mildew resistance loci, Ren6 and Ren7, from the wild Chinese grape species Vitis piasezkii

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The Francis Crick Institute

eScholarship - University of California

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The Francis Crick Institute

The Francis Crick Institute

Springer - Publisher Connector

eScholarship - University of California