Background. Despite its wide distribution worldwide, only 4.6% of temperate grasslands are includedwithin systems of protected areas. In Argentina, this situation is even more alarming: only 1.05% isprotected. The study area (central area of the southern Salado River basin) has a large extent ofgrasslands of Paspalum quadrifarium (Pq) which has been target since the last century of a variety ofagricultural management practices including fire burning for cattle grazing.Methods. Were used as base data bynary images of presence-ausence data of Pq coming from a 42-year (1974-2016) land cover change study performed over Landsat Imagery (MSS, TM, ETM, and OLIsensors). MSPA (Morphological Spatial Pattern Analysis) and Network Analysis were performed to thedata using Guidos Toolbox for the estimation of habitat and connectivity dynamics of the Pq patches(fragments).Results. Was observed a loss of area and habitat nuclei of this grassland between the beginning and theend of the study period. A drastic reduction in connectivity was also evident in resulting maps. Thenumber of large Pq grassland fragments (> 50 ha) decreased during the study period, and fragmentationmeasured as number of components (patches) was higher at the end of study period. The Pq pajonalnuclei had their minimum representativeness in 2000, and recovered slightly in 2011, but with asignificant percentage increase of the small patches (=islets) and linear elements as bridges andbranches. Large corridors (mainly edge of roads) could be observed at the end of study period, while thetotal connectivity of the landscape pattern drops abruptly.Discussion. The habitat reduction could have an impact on the ecosystem functioning and the mobilityof some species of native fauna. The connecting elements of the landscape were maintained and/orrecovered in percentage in 2011 and 2016. This fact, although favoring the dispersion of the presentdiversity in the habitat nuclei could cause degradation by an edge effect. On the methodological side, theuse of a proved tool as Guidos Toolbox for evaluating forest fragmentation could also be useful formonitoring dynamics of a grassland-habitat fragmentation.Fil: Gandini, Marcelo Luciano. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Lara, Bruno Daniel. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; ArgentinaFil: Moreno, Laura Beatriz. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Cañibano, María Alejandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Gandini, Patricia Alejandra. Universidad Nacional de la Patagonia Austral. Unidad Académica Caleta Olivia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin
