C Jaekel

D Brech

E Noessner

M Irmler

P Bruns

T Straub

Journal for ImmunoTherapy of Cancer

PubMed

Springer - Publisher Connector

P31. Genomic profiling and functional characterisation of a new myeloid cell type enriched in renal cell carcinoma

Crossref

POSTER PRESENTATION Open Access
P31. Genomic profiling and functional
characterisation of a new myeloid cell type
enriched in renal cell carcinoma
D Brech1*, M Irmler2, T Straub3, P Bruns4, C Jaekel5, E Noessner1
From 1st Immunotherapy of Cancer Conference (ITOC1)
Munich, Germany. 12-14 March 2014
Dendritic cells (DCs) are central players of the immune
response because they regulate both innate and adaptive
immune cells. As part of the leukocyte infiltrate of various
tumours they can initiate antitumour immune responses
through the activation of tumour-specific T cells.
However, tumours employ strategies to evade the
immune response by manipulating the differentiation
and activation of DCs.
Previously we described ‘enriched-in-renal-carcinoma
DCs’ (ercDCs) as an unusual CD14+CD209+ expressing
myeloid cell type that displays characteristics of both DCs
and macrophages. As one example, ercDCs showed good
antigen cross presentation which is a prototypic DC func-
tion and a prerequisite for the activation of T effector cells
in local tumour tissue. To better understand the relationship
of ercDCs to other myeloid cell types and to get insight in
functional pathways operative in ercDCs we performed tran-
scriptome analysis using Affymetrix® gene arrays comparing
ercDCs, cDCs, proinflammatory M1 and GM-CSF macro-
phages and alternatively activated M2 macrophages. Cell
types were generated in parallel from monocytes of 15
healthy donors. RNA was extracted using the Qiagen®
RNeasy Micro kit. To reduce population variability, RNA
from each cell type- from 5 donors- was pooled and 3 biolo-
gical replicates generated. Clustering of normalised values
showed that ercDCs differ from the other myeloid cell types.
Further analysis included heat mapping and creation of sig-
naling pathways using pathvisio software. Focusing on the
cross presentation pathway, a gene list was assembled
through literature mining and each gene was assigned an
expression value using microarray data. A comparison using
the heat map representation revealed that the expression
pattern of ercDCs was most similar to GM-CSF macro-
phages and least to M1 macrophages. Comparison based on
fold changes generated by normalisation to cDCs revealed
highest similarity between ercDCs and M2 macrophages.
Particularly, transcription of genes involved in receptor-
mediated endocytosis and the vacuolar pathway were upre-
gulated in ercDCs indicative that cross presentation could
be active in ercDCs.
Authors’ details
1Helmholtz Zentrum Muenchen, Institute of Molecular Immunology, Munich,
Germany. 2Helmholtz Zentrum Muenchen, Institute of Experimental Genetics,
Munich, Germany. 3Ludwig-Maximilians-University, Adolf Budenandt Institute,
Munich, Germany. 4Helmholtz Zentrum Muenchen, Institute of Bioinformatics
and Systems Biology, Munich, Germany. 5Ludwig-Maximilians-University,
Clinical Biochemistry, Munich, Germany.
Published: 12 March 2014
doi:10.1186/2051-1426-2-S2-P22
Cite this article as: Brech et al.: P31. Genomic profiling and functional
characterisation of a new myeloid cell type enriched in renal cell
carcinoma. Journal for ImmunoTherapy of Cancer 2014 2(Suppl 2):P22.
Submit your next manuscript to BioMed Central
and take full advantage of: 
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at 
www.biomedcentral.com/submit
1Helmholtz Zentrum Muenchen, Institute of Molecular Immunology, Munich,
Germany
Full list of author information is available at the end of the article
Brech et al. Journal for ImmunoTherapy of Cancer 2014, 2(Suppl 2):P22
http://www.immunotherapyofcancer.org/content/2/S2/P22
© 2014 Brech et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://
creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


http://dx.doi.org/10.1186/2051-1426-2-S2-P22

P31. Genomic profiling and functional characterisation of a new myeloid cell type enriched in renal cell carcinoma

Abstract

Similar works

Full text

Available Versions

Springer - Publisher Connector

Crossref

Springer - Publisher Connector