Ala Cojocaru

Dorina Savoschin

Dumitru Amoasii

Liubov Vasilos

Mikhail Aseev

Olga Cirstea

Tatiana Ivaschenko

Clinical and Translational Allergy

PubMed

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POSTER DISCUSSION PRESENTATION Open Access
PD09 - Association of the TNFa -308 G>A
polymorphism with clinical phenotypes of asthma
in Moldovan children
Olga Cirstea1*, Liubov Vasilos1, Ala Cojocaru1, Dorina Savoschin1, Tatiana Ivaschenko2, Mikhail Aseev2,
Dumitru Amoasii3
From 3rd Pediatric Allergy and Asthma Meeting (PAAM)
Athens, Greece. 17-19 October 2013
Background
Patients with asthma show a marked phenotypic variabil-
ity, suggesting etiological heterogeneity and strong envir-
onmental influences. Bronchial asthma is determined by
a complex interaction between genetic and environmental
factors, but these mechanisms are not yet elucidated.
The aim of the study was to evaluate peculiarities of the
TNFa gene polymorphism (-308 G>A) incidence and its
association with different clinical evolution of bronchial
asthma in Moldovan children.
Material and methods
TNFa gene polymorphism (-308 G>A) was studied in 180
Moldavian children in order to assess the prevalence of
the allelic variants of the gene in 90 children with asthma
and 90 controls. All the patients underwent complex
clinical and functional examination (spirography with
bronchodilatational test), and laboratory evaluation
(molecular genetics, immunological and general tests).
Results
Following genotypes of the TNFa gene were identified -
TNFa -308 G/G, TNFa -308 G/A and TNFa -308 A/A.
The study showed that the homozygous genotype TNFa
-308 G/G has protective role, being significantly more
frequently identified in children with solitary form of
asthma compared with those with allergic triad (86.2%
versus 56.4%, respectively; OR = 4.83, 95% CI: 1.41 to
16.54, p<0.05). However, functionally compromised geno-
type TNFa -308 G/A was found more frequently in
children with asthma associated with other allergic
symptoms (ie., 38.5% compared with 13.8% in cases with
asthma alone, p<0.05) and was observed twofold more
frequently in boys with mild asthma with no associated
atopic symptoms, compared to those with moderately to
severe evolution of the disease (40.9% versus 20.7%,
respectively; p> 0.05). Noticeably, the homozygous
genotype TNFa -308 A/A group was identified only in
children with allergic triad in 5.1% of cases.
Conclusion
Study results demonstrate the association of the function-
ally compromised of genotypes of the TNF-a-308 gene
with different phenotypes of asthma in Moldavian
children.
Authors’ details
1Institute for Maternal and Child Healthcare, Chisinau, Moldova Republic.
2Laboratory of Prenatal Diagnosis of Congenital and Inherited Diseases, D.O.
Ott Research Institute, Saint Petersbur, Russia. 3N. Testemitanu State Medical
and Pharmaceutical University, Chisinau, Moldova Republic.
Published: 28 February 2014
doi:10.1186/2045-7022-4-S1-P9
Cite this article as: Cirstea et al.: PD09 - Association of the TNFa -308
G>A polymorphism with clinical phenotypes of asthma in Moldovan
children. Clinical and Translational Allergy 2014 4(Suppl 1):P9.
1Institute for Maternal and Child Healthcare, Chisinau, Moldova Republic
Full list of author information is available at the end of the article
Cirstea et al. Clinical and Translational Allergy 2014, 4(Suppl 1):P9
http://www.ctajournal.com/content/4/S1/P9
© 2014 Cirstea et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction
in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.


PD09 - Association of the TNFα -308 G>A polymorphism with clinical phenotypes of asthma in Moldovan children

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PD09 - Association of the TNFα -308 G>A polymorphism with clinical phenotypes of asthma in Moldovan children

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