CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
Expression profile of telomere-associated genes in multiple myeloma
Authors
Verónica Ayllón
Purificación Catalina
+8 more
Rafael Díaz De la Guardia
Carolina Elosua
Paola E. Leone
Gertrudis Ligero
María Belén López
Julieta Panero
Andrés Pulgarin
Irma Rosa Slavutsky
Publication date
1 December 2012
Publisher
'Wiley'
Doi
Cite
View
on
PubMed
Abstract
To further contribute to the understanding of multiple myeloma, we have focused our research interests on the mechanisms by which tumour plasma cells have a higher survival rate than normal plasma cells. In this article, we study the expression profile of genes involved in the regulation and protection of telomere length, telomerase activity and apoptosis in samples from patients with monoclonal gammopathy of undetermined significance, smouldering multiple myeloma, multiple myeloma (MM) and plasma cell leukaemia (PCL), as well as several human myeloma cell lines (HMCLs). Using conventional cytogenetic and fluorescence in situ hybridization studies, we identified a high number of telomeric associations (TAs). Moreover, telomere length measurements by terminal restriction fragment (TRF) assay showed a shorter mean TRF peak value, with a consistent correlation with the number of TAs. Using gene expression arrays and quantitative PCR we identified the hTERT gene together with 16 other genes directly involved in telomere length maintenance: HSPA9, KRAS, RB1, members of the Small nucleolar ribonucleoproteins family, A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins, and 14-3-3 family. The expression levels of these genes were even higher than those in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), which have unlimited proliferation capacity. In conclusion, the gene signature suggests that MM tumour cells are able to maintain stable short telomere lengths without exceeding the short critical length, allowing cell divisions to continue. We propose that this could be a mechanism contributing to MM tumour cells expansion in the bone marrow (BM). © 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.Fil: De la Guardia, Rafael Díaz. Universidad de Granada; EspañaFil: Catalina, Purificación. Universidad de Granada; EspañaFil: Panero, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Elosua, Carolina. Universidad de Granada; EspañaFil: Pulgarin, Andrés. Universidad de Granada; EspañaFil: López, María Belén. Universidad de Granada; EspañaFil: Ayllón, Verónica. Universidad de Granada; EspañaFil: Ligero, Gertrudis. Universidad de Granada; EspañaFil: Slavutsky, Irma Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Leone, Paola E.. Universidad de Granada; Españ
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
CONICET Digital
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:ri.conicet.gov.ar:11336/52...
Last time updated on 21/09/2018
Crossref
See this paper in CORE
Go to the repository landing page
Download from data provider
info:doi/10.1111%2Fj.1582-4934...
Last time updated on 02/01/2020
LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:ri.conicet.gov.ar:11336/52...
Last time updated on 18/05/2022