Escherichia coli O157:H7 is a major etiologic agent of diseases in humans that cause diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome. Here, we report the draft genome sequences of two strains isolated from cattle that had high levels of Shiga toxin 2 and high lethality in mice.Fil: Amadio, Ariel Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Amigo, Natalia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Puebla, Andrea Fabiana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentin

Amadio, Ariel Fernando

Amigo, Natalia

Cataldi, Ángel Adrián

Farber, Marisa Diana

Puebla, Andrea Fabiana

Genome Announcements

English

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Draft Genome Sequences of Escherichia coli O157:H7 StrainsRafaela_II (Clade 8) and 7.1_Anguil (Clade 6) from Cattle inArgentinaAriel Fernando Amadio,a,c Natalia Amigo,b,c Andrea Fabiana Puebla,b Marisa Diana Farber,b,c Angel Adrián Cataldib,cEstación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA), Rafaela, Santa Fe, Argentinaa; Centro de Investigación en CienciasVeterinarias y Agronómicas (CICVyA), Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentinab; ConsejoNacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinacEscherichia coli O157:H7 is a major etiologic agent of diseases in humans that cause diarrhea, hemorrhagic colitis, andhemolytic-uremic syndrome. Here, we report the draft genome sequences of two strains isolated from cattle that had high levelsof Shiga toxin 2 and high lethality in mice.Received 11 May 2015 Accepted 13 May 2015 Published 11 June 2015Citation Amadio AF, Amigo N, Puebla AF, Farber MD, Cataldi AA. 2015. Draft genome sequences of Escherichia coli O157:H7 strains Rafaela_II (clade 8) and 7.1_Anguil (clade 6)from cattle in Argentina. Genome Announc 3(3):e00617-15. doi:10.1128/genomeA.00617-15.Copyright © 2015 Amadio et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.Address correspondence to Angel Adrián Cataldi, cataldi.angeladrian@inta.gob.ar.Escherichia coli O157:H7 is the etiologic agent of hemolytic-uremic syndrome. This is the leading cause of chronic renalfailure in children in Argentina and several other countries (1).This bacterium produces Shiga toxin (Stx) types 1 and 2 (2, 3),which are responsible for systemic damage. The main reservoir ofE. coli O157:H7 is cattle, which harbor this organism in their in-testinal tract (4, 5), especially on the lymphoid follicle-dense mu-cosa at the terminal rectum (6). Fecal contamination of meat dur-ing slaughter, the use of feces as fertilizer, and the contaminationof drinking water (5, 7) cause the entry of these bacteria into thehuman food chain. Single nucleotide polymorphism (SNP) typingwas previously used to define nine E. coli O157:H7 clades, and theclade 8 strains were found to be more associated with severe dis-ease (8).This work reports the draft genome sequences of two E. coliO157:H7 strains isolated from cattle in the central humid Pampas,Argentina, in 2009. Strain Rafaela_II belongs to clade 8, and strain7.1_Anguil belongs to clade 6. Both strains produced elevated lev-els of Shiga toxin 2 and had high lethality in mice (N. Amigo, E.Mercado, A. Bentancor, P. Singh, D. Vilte, E. Gerhardt, E. Zotta,C. Ibarra, S. D. Manning, M. Larzábal, and A. Cataldi, unpub-lished data).Genomic DNA was isolated using a standard chloroform-isoamilic alcohol extraction. Paired-end Nextera XT libraries wereconstructed (500-bp insert size) and sequenced in an IlluminaMiSeq sequencer (2  250 bp). Raw sequences were qualitytrimmed with Sickle (9), resulting in 1,262,211 and 1,048,969 se-quences for 7.1_Anguil and Rafaela_II, respectively. De novo as-sembly was done using SPAdes version 3.1.0 (10). For 7.1_Anguil,244 scaffolds 500 bp were obtained, the largest being 375,850 bp,with an N50 of 146,432 bp and a GC content of 50.46%. ForRafaela_II, 232 scaffolds 500 bp were obtained, the largest being375,042 bp, with an N50 of 147,588 bp and a GC content of50.28%. Scaffolds were ordered using ABACAS (11) and com-pared with the genomes of E. coli O157:H7 strains Tw14359 (12)and EDL933 (13) using BLAST and ACT (14) to analyze structuraldifferences. Variants at the nucleotide level were analyzed usingbreseq (15) and the same references. Both strains carry the largevirulence plasmid pO157, but strain 7.1_Anguil has an additionalplasmid highly homologous to avian pathogenic E. coli (APEC)strain 7122 (O78:K80:H9) plasmid pChi7122-3.Genes were predicted with Prodigal version 2.6.1 (16), obtain-ing 5,643 and 5,439 genes for 7.1_Anguil and Rafaela_II, respec-tively. Using GET_HOMOLOGUES (17), we compared the genecontent using the OrthoMCL methodology (18) to obtain sharedand unique genes using strains TW14359 and EDL933 as refer-ences. The comparison of shared genes showed a closer phyloge-netic relationship between isolate 7.1_Anguil and reference strainEDL933, while isolate Rafaela_II is closely related to TW14359.This is also supported by a previously performed SNP analysis(15). Based on core and pangenome analysis, a considerable num-ber of exclusive genes from Rafaela_II (272) and 7.1_Anguil (447)are under analysis.Nucleotide sequence accession numbers. These whole-genome shotgun (WGS) projects have been deposited at DDBJ/EMBL/GenBank under the accession numbers LAZD00000000for 7.1_Anguil and LAYW00000000 for Rafaela_II. The versionsdescribed in this paper are LAZD01000000 and LAYW01000000,respectively.ACKNOWLEDGMENTSThis work was supported by FONCYT (PICT-2012-0211) and INTA(PNBIO-1131043).REFERENCES1. Karch H, Tarr PI, Bielaszewska M. 2005. Enterohaemorrhagic Esche-richia coli in human medicine. Int J Med Microbiol 295:405– 418. http://dx.doi.org/10.1016/j.ijmm.2005.06.009.2. Jackson MP, Neill RJ, O’Brien AD, Holmes RK, Newland JW. 1987.Nucleotide sequence analysis and comparison of the structural genes forShiga-like toxin I and Shiga-like toxin II encoded by bacteriophages fromcrossmarkGenome AnnouncementsMay/June 2015 Volume 3 Issue 3 e00617-15 genomea.asm.org 1Escherichia coli 933. FEMS Microbiol Lett 44:109 –114. http://dx.doi.org/10.1111/j.1574-6968.1987.tb02252.x.3. Tesh VL, O’Brien AD. 1991. The pathogenic mechanisms of Shiga toxinand the Shiga-like toxins. Mol Microbiol 5:1817–1822. http://dx.doi.org/10.1111/j.1365-2958.1991.tb00805.x.4. Meichtri L, Miliwebsky E, Gioffré A, Chinen I, Baschkier A, Chillemi G,Guth BE, Masana MO, Cataldi A, Rodríguez HR, Rivas M. 2004. Shigatoxin-producing Escherichia coli in healthy young beef steers fromArgentina: prevalence and virulence properties. Int J Food Microbiol 96:189 –198. http://dx.doi.org/10.1016/j.ijfoodmicro.2004.03.018.5. Hussein HS, Bollinger LM. 2005. Prevalence of Shiga toxin-producingEscherichia coli in beef cattle. J Food Prot 68:2224 –2241.6. Grauke LJ, Kudva IT, Yoon JW, Hunt CW, Williams CJ, Hovde CJ.2002. Gastrointestinal tract location of Escherichia coli O157:H7 in rumi-nants. Appl Environ Microbiol 68:2269 –2277. http://dx.doi.org/10.1128/AEM.68.5.2269-2277.2002.7. Olsen SJ, Miller G, Breuer T, Kennedy M, Higgins C, Walford J, McKeeG, Fox K, Bibb W, Mead P. 2002. A waterborne outbreak of Escherichiacoli O157:H7 infections and hemolytic uremic syndrome: implications forrural water systems. Emerg Infect Dis 8:370 –375. http://dx.doi.org/10.3201/eid0804.000218.8. Manning SD, Motiwala AS, Springman AC, Qi W, Lacher DW, Ouel-lette LM, Mladonicky JM, Somsel P, Rudrik JT, Dietrich SE, Zhang W,Swaminathan B, Alland D, Whittam TS. 2008. Variation in virulenceamong clades of Escherichia coli O157:H7 associated with disease out-breaks. Proc Natl Acad Sci U S A 105:4868 – 4873. http://dx.doi.org/10.1073/pnas.0710834105.9. Joshi NA, Fass JN. 2011. Sickle: a sliding-window, adaptive, quality-basedtrimming tool for FastQ files (version 1.33). https://github.com/najoshi/sickle.10. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS,Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV,Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA. 2012. SPAdes: a newgenome assembly algorithm and its applications to single-cell sequencing. JComput Biol 19:455–477. http://dx.doi.org/10.1089/cmb.2012.0021.11. Assefa S, Keane TM, Otto TD, Newbold C, Berriman M. 2009. ABACAS:algorithm-based automatic contiguation of assembled sequences. Bioinfor-matics 25:1968–1969. http://dx.doi.org/10.1093/bioinformatics/btp347.12. Kulasekara BR, Jacobs M, Zhou Y, Wu Z, Sims E, Saenphimmachak C,Rohmer L, Ritchie JM, Radey M, McKevitt M, Freeman TL, Hayden H,Haugen E, Gillett W, Fong C, Chang J, Beskhlebnaya V, Waldor MK,Samadpour M, Whittam TS, Kaul R, Brittnacher M, Miller SI. 2009.Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-associated outbreak isolate indicates candidate genes that may enhancevirulence. Infect Immun 77:3713–3721. http://dx.doi.org/10.1128/IAI.00198-09.13. Latif H, Li HJ, Charusanti P, Palsson BØ, Aziz RK. 2014. A gapless,unambiguous genome sequence of the enterohemorrhagic Escherichia coliO157:H7 strain EDL933. Genome Announc 2(4):e00821-14. http://dx.doi.org/10.1128/genomeA.00821-14.14. Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG,Parkhill J. 2005. ACT: the Artemis comparison tool. Bioinformatics 21:3422–3423. http://dx.doi.org/10.1093/bioinformatics/bti553.15. Barrick JE, Colburn G, Deatherage DE, Traverse CC, Strand MD,Borges JJ, Knoester DB, Reba A, Meyer AG. 2014. Identifying structuralvariation in haploid microbial genomes from short-read resequencingdata using breseq. BMC Genomics 15:1039. http://dx.doi.org/10.1186/1471-2164-15-1039.16. Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ.2010. Prodigal: prokaryotic gene recognition and translation initiation siteidentification. BMC Bioinformatics 11:119. http://dx.doi.org/10.1186/1471-2105-11-119.17. Contreras-Moreira B, Vinuesa P. 2013. GET_HOMOLOGUES, a versa-tile software package for scalable and robust microbial pangenome anal-ysis. Appl Environ Microbiol 79:7696 –7701. http://dx.doi.org/10.1128/AEM.02411-13.18. Li L, Stoeckert CJ, Jr, Roos DS. 2003. OrthoMCL: identification ofortholog groups for eukaryotic genomes. Genome Res 13:2178 –2189.http://dx.doi.org/10.1101/gr.1224503.Amadio et al.Genome Announcements2 genomea.asm.org May/June 2015 Volume 3 Issue 3 e00617-15

Draft genome sequences of Escherichia coli O157: H7 strains Rafaela_II (clade 8) and 7.1_Anguil (clade 6) from cattle in Argentina

CONICET Digital

Draft Genome Sequences of Escherichia coli O157:H7 StrainsRafaela_II (Clade 8) and 7.1_Anguil (Clade 6) from Cattle inArgentinaAriel Fernando Amadio,a,c Natalia Amigo,b,c Andrea Fabiana Puebla,b Marisa Diana Farber,b,c Angel Adrián Cataldib,cEstación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA), Rafaela, Santa Fe, Argentinaa; Centro de Investigación en CienciasVeterinarias y Agronómicas (CICVyA), Instituto de Biotecnología, Instituto Nacional de Tecnología Agropecuaria (INTA), Hurlingham, Buenos Aires, Argentinab; ConsejoNacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinacEscherichia coliO157:H7 is a major etiologic agent of diseases in humans that cause diarrhea, hemorrhagic colitis, andhemolytic-uremic syndrome. Here, we report the draft genome sequences of two strains isolated from cattle that had high levelsof Shiga toxin 2 and high lethality in mice.Received 11 May 2015 Accepted 13 May 2015 Published 11 June 2015Citation Amadio AF, Amigo N, Puebla AF, Farber MD, Cataldi AA. 2015. Draft genome sequences of Escherichia coli O157:H7 strains Rafaela_II (clade 8) and 7.1_Anguil (clade 6)from cattle in Argentina. Genome Announc 3(3):e00617-15. doi:10.1128/genomeA.00617-15.Copyright © 2015 Amadio et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.Address correspondence to Angel Adrián Cataldi, cataldi.angeladrian@inta.gob.ar.Escherichia coli O157:H7 is the etiologic agent of hemolytic-uremic syndrome. This is the leading cause of chronic renalfailure in children in Argentina and several other countries (1).This bacterium produces Shiga toxin (Stx) types 1 and 2 (2, 3),which are responsible for systemic damage. The main reservoir ofE. coli O157:H7 is cattle, which harbor this organism in their in-testinal tract (4, 5), especially on the lymphoid follicle-dense mu-cosa at the terminal rectum (6). Fecal contamination of meat dur-ing slaughter, the use of feces as fertilizer, and the contaminationof drinking water (5, 7) cause the entry of these bacteria into thehuman food chain. Single nucleotide polymorphism (SNP) typingwas previously used to define nine E. coliO157:H7 clades, and theclade 8 strains were found to be more associated with severe dis-ease (8).This work reports the draft genome sequences of two E. coliO157:H7 strains isolated from cattle in the central humid Pampas,Argentina, in 2009. Strain Rafaela_II belongs to clade 8, and strain7.1_Anguil belongs to clade 6. Both strains produced elevated lev-els of Shiga toxin 2 and had high lethality in mice (N. Amigo, E.Mercado, A. Bentancor, P. Singh, D. Vilte, E. Gerhardt, E. Zotta,C. Ibarra, S. D. Manning, M. Larzábal, and A. Cataldi, unpub-lished data).Genomic DNA was isolated using a standard chloroform-isoamilic alcohol extraction. Paired-endNexteraXT libraries wereconstructed (500-bp insert size) and sequenced in an IlluminaMiSeq sequencer (2  250 bp). Raw sequences were qualitytrimmed with Sickle (9), resulting in 1,262,211 and 1,048,969 se-quences for 7.1_Anguil and Rafaela_II, respectively. De novo as-sembly was done using SPAdes version 3.1.0 (10). For 7.1_Anguil,244 scaffolds500 bpwere obtained, the largest being 375,850 bp,with an N50 of 146,432 bp and a GC content of 50.46%. ForRafaela_II, 232 scaffolds500 bp were obtained, the largest being375,042 bp, with an N50 of 147,588 bp and a GC content of50.28%. Scaffolds were ordered using ABACAS (11) and com-pared with the genomes of E. coli O157:H7 strains Tw14359 (12)and EDL933 (13) using BLAST andACT (14) to analyze structuraldifferences. Variants at the nucleotide level were analyzed usingbreseq (15) and the same references. Both strains carry the largevirulence plasmid pO157, but strain 7.1_Anguil has an additionalplasmid highly homologous to avian pathogenic E. coli (APEC)strain 7122 (O78:K80:H9) plasmid pChi7122-3.Genes were predicted with Prodigal version 2.6.1 (16), obtain-ing 5,643 and 5,439 genes for 7.1_Anguil and Rafaela_II, respec-tively. Using GET_HOMOLOGUES (17), we compared the genecontent using the OrthoMCL methodology (18) to obtain sharedand unique genes using strains TW14359 and EDL933 as refer-ences. The comparison of shared genes showed a closer phyloge-netic relationship between isolate 7.1_Anguil and reference strainEDL933, while isolate Rafaela_II is closely related to TW14359.This is also supported by a previously performed SNP analysis(15). Based on core and pangenome analysis, a considerable num-ber of exclusive genes fromRafaela_II (272) and 7.1_Anguil (447)are under analysis.Nucleotide sequence accession numbers. These whole-genome shotgun (WGS) projects have been deposited at DDBJ/EMBL/GenBank under the accession numbers LAZD00000000for 7.1_Anguil and LAYW00000000 for Rafaela_II. The versionsdescribed in this paper are LAZD01000000 and LAYW01000000,respectively.ACKNOWLEDGMENTSThis work was supported by FONCYT (PICT-2012-0211) and INTA(PNBIO-1131043).REFERENCES1. Karch H, Tarr PI, Bielaszewska M. 2005. Enterohaemorrhagic Esche-richia coli in human medicine. Int J Med Microbiol 295:405–418. http://dx.doi.org/10.1016/j.ijmm.2005.06.009.2. Jackson MP, Neill RJ, O’Brien AD, Holmes RK, Newland JW. 1987.Nucleotide sequence analysis and comparison of the structural genes forShiga-like toxin I and Shiga-like toxin II encoded by bacteriophages fromcrossmarkGenome AnnouncementsMay/June 2015 Volume 3 Issue 3 e00617-15 genomea.asm.org 1Escherichia coli 933. FEMS Microbiol Lett 44:109–114. http://dx.doi.org/10.1111/j.1574-6968.1987.tb02252.x.3. Tesh VL, O’Brien AD. 1991. The pathogenic mechanisms of Shiga toxinand the Shiga-like toxins. Mol Microbiol 5:1817–1822. http://dx.doi.org/10.1111/j.1365-2958.1991.tb00805.x.4. Meichtri L, Miliwebsky E, Gioffré A, Chinen I, Baschkier A, Chillemi G,Guth BE, Masana MO, Cataldi A, Rodríguez HR, Rivas M. 2004. Shigatoxin-producing Escherichia coli in healthy young beef steers fromArgentina: prevalence and virulence properties. Int J Food Microbiol 96:189–198. http://dx.doi.org/10.1016/j.ijfoodmicro.2004.03.018.5. Hussein HS, Bollinger LM. 2005. Prevalence of Shiga toxin-producingEscherichia coli in beef cattle. J Food Prot 68:2224–2241.6. Grauke LJ, Kudva IT, Yoon JW, Hunt CW, Williams CJ, Hovde CJ.2002. Gastrointestinal tract location of Escherichia coli O157:H7 in rumi-nants. Appl EnvironMicrobiol 68:2269–2277. http://dx.doi.org/10.1128/AEM.68.5.2269-2277.2002.7. Olsen SJ, Miller G, Breuer T, Kennedy M, Higgins C, Walford J, McKeeG, Fox K, Bibb W, Mead P. 2002. A waterborne outbreak of EscherichiacoliO157:H7 infections and hemolytic uremic syndrome: implications forrural water systems. Emerg Infect Dis 8:370–375. http://dx.doi.org/10.3201/eid0804.000218.8. Manning SD, Motiwala AS, Springman AC, Qi W, Lacher DW, Ouel-lette LM, Mladonicky JM, Somsel P, Rudrik JT, Dietrich SE, Zhang W,Swaminathan B, Alland D, Whittam TS. 2008. Variation in virulenceamong clades of Escherichia coli O157:H7 associated with disease out-breaks. Proc Natl Acad Sci U S A 105:4868–4873. http://dx.doi.org/10.1073/pnas.0710834105.9. Joshi NA, Fass JN. 2011. Sickle: a sliding-window, adaptive, quality-basedtrimming tool for FastQ files (version 1.33). https://github.com/najoshi/sickle.10. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS,Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV,Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA. 2012. SPAdes: a newgenome assembly algorithm and its applications to single-cell sequencing. JComput Biol 19:455–477. http://dx.doi.org/10.1089/cmb.2012.0021.11. Assefa S, Keane TM, Otto TD, Newbold C, Berriman M. 2009. ABACAS:algorithm-based automatic contiguation of assembled sequences. Bioinfor-matics 25:1968–1969. http://dx.doi.org/10.1093/bioinformatics/btp347.12. Kulasekara BR, Jacobs M, Zhou Y, Wu Z, Sims E, Saenphimmachak C,Rohmer L, Ritchie JM, Radey M, McKevitt M, Freeman TL, Hayden H,Haugen E, Gillett W, Fong C, Chang J, Beskhlebnaya V, Waldor MK,Samadpour M, Whittam TS, Kaul R, Brittnacher M, Miller SI. 2009.Analysis of the genome of the Escherichia coli O157:H7 2006 spinach-associated outbreak isolate indicates candidate genes that may enhancevirulence. Infect Immun 77:3713–3721. http://dx.doi.org/10.1128/IAI.00198-09.13. Latif H, Li HJ, Charusanti P, Palsson BØ, Aziz RK. 2014. A gapless,unambiguous genome sequence of the enterohemorrhagic Escherichia coliO157:H7 strain EDL933. Genome Announc 2(4):e00821-14. http://dx.doi.org/10.1128/genomeA.00821-14.14. Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG,Parkhill J. 2005. ACT: the Artemis comparison tool. Bioinformatics 21:3422–3423. http://dx.doi.org/10.1093/bioinformatics/bti553.15. Barrick JE, Colburn G, Deatherage DE, Traverse CC, Strand MD,Borges JJ, Knoester DB, Reba A, Meyer AG. 2014. Identifying structuralvariation in haploid microbial genomes from short-read resequencingdata using breseq. BMC Genomics 15:1039. http://dx.doi.org/10.1186/1471-2164-15-1039.16. Hyatt D, Chen GL, Locascio PF, Land ML, Larimer FW, Hauser LJ.2010. Prodigal: prokaryotic gene recognition and translation initiation siteidentification. BMC Bioinformatics 11:119. http://dx.doi.org/10.1186/1471-2105-11-119.17. Contreras-Moreira B, Vinuesa P. 2013. GET_HOMOLOGUES, a versa-tile software package for scalable and robust microbial pangenome anal-ysis. Appl Environ Microbiol 79:7696–7701. http://dx.doi.org/10.1128/AEM.02411-13.18. Li L, Stoeckert CJ, Jr, Roos DS. 2003. OrthoMCL: identification ofortholog groups for eukaryotic genomes. Genome Res 13:2178–2189.http://dx.doi.org/10.1101/gr.1224503.Amadio et al.Genome Announcements2 genomea.asm.org May/June 2015 Volume 3 Issue 3 e00617-15

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Draft genome sequences of Escherichia coli O157: H7 strains Rafaela_II (clade 8) and 7.1_Anguil (clade 6) from cattle in Argentina

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