Sequence comparisons of U2 snRNA genes and flanking regions from T. cruzi (CL Brener Non-Esmeraldo-like). Sequences from the genes located on chromosomes 23, 37 and 6 are shown. The U2 snRNA gene from chromosome 23 is presented in blue font. The position of boxes A and B is indicated. Sequence numbers are relative to the TSS (+1) from the U2 snRNA. (PDF 1404 kb

Elisa Figueroa-Angulo

Luis E. Florencio-Martínez

Rebeca G. Manning-Cela

Rodrigo Moreno-Campos

Santiago Martínez-Calvillo

Saúl Rojas-Sánchez

Parasites & Vectors

Northern blot analysis of the tagged U2 snRNA. The experiment was performed with total RNA from cells transfected with construct 1 (pComp), construct 6 (pBS + 6/+12), the unrelated vector pLMRIB or mock-transfected cells. The probe was the oligonucleotide LmjU2tag-Rev, which specifically recognizes the tag sequence. (TIF 807 kb

Saúl Rojas-Sánchez (3433325)

Elisa Figueroa-Angulo (3433319)

Rodrigo Moreno-Campos (3433328)

Luis Florencio-Martínez (3433331)

Rebeca Manning-Cela (3433322)

Santiago Martínez-Calvillo (3433334)

The Francis Crick Institute

Additional file 2: Figure S2. of Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene

Mutations introduced in the tRNA-Ala/U2 snRNA locus. For each vector, sequence elements are indicated inside colored boxes: tRNA-Ala in green, tRNA-like in orange and U2 snRNA in blue. Boxes A and B are also indicated. The tag sequence, inserted within the U2 snRNA gene, is also shown. Base substitutions are indicated in red font. (PDF 3122 kb

Additional file 3: Figure S3. of Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene

Sequence alignment of U2 snRNA genes from trypanosomatids. Sequences were obtained from the TriTrypDB databases of the following species of trypanosomatids: L. major Friedlin (Lmj), L. arabica LEM1108 (Lar), L. tarentolae Parrot-TarII (Ltr), L. infantum JPCM5 (Lin), L. amazonensis MHOMBR/71973/M2269 (Laz), L. donovani BPK282A1 (Ldn), L. aethiopica L147 (Lae), L. mexicana U1103 (Lmx), L. panamensis MHOM/COL/81/L13 (Lpn), L. braziliensis M2904 (Lbr), L. enriettii LEM3045 (Len), E. monterogeii LV88 (Emg), C. fasciculata Cf-Cl (Cfs), L. pyrrhocoris H10 (Lpy), L. seymouri ATCC 30220 (Lsy), L. collosoma (unspecified strain) [39] (Lcl), T. cruzi CL Brener Non-Esmeraldo-like, copy of the chromosome 23 (Tcr), T. vivax Y486 (Tvx), T. congolense IL3000 (Tcn), T. evansi STIB 805 (Tev) and T. brucei DAL972 (Tbr). The alignment was obtained with the DNAMAN software, and manually corrected. Arrows indicate the identified pseudouridines (Ψ) in the L. major U2 snRNA (this work). BPRR and Sm sites are also indicated. The 3' ends (C residues) that have been experimentally mapped are shown in red font for L. major (this work), T. cruzi [45] and T. brucei [44]. The T-tract length is indicated for each species. Sequence numbers are relative to the TSS (+1) from the U2 snRNA. This alignment was used to generate the sequence logo depicted in Fig. 3b. (PDF 2137 kb

Additional file 1: Figure S1. of Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene

Predicted secondary structure of the tRNA-Ala (panel a) and the tRNA-like associated to the L. major U2 snRNA gene (panel b). The minimum free energy structures are shown. The color scale indicates low (blue) to high (red) probabilities of base pairing. (PDF 355 kb

Additional file 4: Figure S4. of Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene

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Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene

Additional file 5: Figure S5. of Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene

Genomic context of U2 snRNA genes in trypanosomatids. Schematic representations of the U2 snRNA loci from Leishmania spp., E. monterogeii (LV88), L. seymouri (ATCC 30220), C. fasciculata (Cf-Cl), T. cruzi (CL Brener Non-Esmeraldo-like, copy of the chromosome 23) and T. brucei (DAL972). All of the U2 snRNA genes contain divergently-oriented boxes A and B at conserved distances (around 104 bases), which are contained in tRNA-like sequences. The exception is C. fasciculata, where boxes A and B are located inside a tRNA-Ala gene. In T. cruzi, a tRNA-Arg gene is located downstream of the U2 snRNA gene. Figure is drawn to scale. (TIF 268 kb

Additional file 6: Figure S6. of Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene

https://figshare.com/articles/Additional_file_6_Figure_S6_of_Transcription_of_Leishmania_major_U2_small_nuclear_RNA_gene_is_directed_by_extragenic_sequences_located_within_a_tRNA-like_and_a_tRNA-Ala_gene/4341536

Transcription of Leishmania major U2 small nuclear RNA gene is directed by extragenic sequences located within a tRNA-like and a tRNA-Ala gene

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Springer - Publisher Connector

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The Francis Crick Institute

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